"PCR Lab Activities"

Ideal for Beginning PCR Instruction

This Promega offers enhanced amplification over conventional Taq DNA polymerases. Supplied with 5X colorless reaction buffer and a 5X green buffer, which both allow for direct loading into agarose gels with no loading dye required. The included 5X green buffer allows you to visualize migration during electrophoresis. Each tube contains 100 U at 5 U/μl. Note: Requires storage at − 20°C.

Study Genetic Variants in Fruit Flies

Pre-mixed, and ready to use, this master mix contains DNA polymerase, dNTPs, MgCl₂, and reaction buffers at optimal concentrations for the amplification of DNA via PCR. Its special formulation allows for direct loading of PCR samples into agarose gels as well as tracking the migration during electrophoresis without the need for loading dyes. Simply add template, primers, and nuclease-free water, available separately. Mater Mix reduces risk of contamination. Includes enough for 100 reactions. Note: Can be stored at 4 °C for up to six weeks, or −20 °C for longer.

Complete Three Modules Using Lamba DNA Template

Introduce Students to PCR Technique with a Basic Procedure

An Interactive DNA Investigation

Use PCR to determine if your parrots are male (ZZ) or female (ZW).

Use PCR to determine if your chickens are male (ZZ) or female (ZW).

Use PCR to determine if your emus are male (ZZ) or female (ZW).

Learn and practice CRISPR and PCR.

Let your students isolate, amplify and visualize their own Mitochondrial DNA.

Detect genetic markers for a disease.

Learn and practice CRISPR.

Apply Biotechnology to Forensics Investigations

Use PCR to genotype your own chickens for the recessive white allele that causes white plumage.