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"Ward's Phenomena"

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Phenol Red Lactose Broth

Phenol Red Lactose Broth

A basal medium containing a single carbohydrate used to determine whether bacteria can ferment the specific carbohydrate. The medium contains a pH indicator that changes from red to yellow if the bacteria can ferment the carbohydrate.

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Starch Agar

Starch Agar

Used to determine if bacteria produce extracellular enzymes to catalyze the hydrolysis of starch. Organisms that produce a starch hydrolyzing enzyme will display a clear zone around the area of growth while other areas of the agar containing starch will turn blue-black.

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Phenol Red Dextrose Broth

Phenol Red Dextrose Broth

To prepare media, add 20 g of phenol red dextrose broth to a liter of distilled or de-ionized water. The media will not be sterile until autoclaved prior to use.

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Phenol Red Sucrose Broth

Phenol Red Sucrose Broth

A basal medium containing a single carbohydrate used to determine whether bacteria can ferment the specific carbohydrate. The medium contains a pH indicator that changes from red to yellow if the bacteria can ferment the carbohydrate.

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