Loop-Mediated Isothermal Amplification (LAMP) Kits

Perform loop-mediated isothermal amplification (LAMP) without the need for expensive equipment, even from home.

• Perform a real DNA amplification Via LAMP-reaction
• Verify presence of LAMP-amplified DNA by color change
• Available with or without a follow-up Electrophoresis step
• Kits comes with lesson on LAMP and follow-up questions

In these activities, students play the role of the diagnostician in the following scenario. Patients arrive at the emergency room with fever and cough. A viral infection is suspected and must be confirmed or ruled-out from patient samples. Pre-amplified plasmid DNA is used to emulate the virus; no actual viral particles are used.

In both kit versions, students will perform a simulated loop-mediated isothermal amplification (LAMP) reaction and will verify DNA amplification occurred by observing a color change. Both kits contain enough reagents for 24 reactions and must be stored in the freezer upon arrival). Simulated LAMP kit is also available elsewhere.

Iso-B kit LAMP Reaction and verification can be done without any specialized equipment other than a micropipette or capillary pipette.

Iso-S Kit LAMP reaction and verification can be done without any specialized equipment other than a micropipette or capillary pipette. This kit also incorporates a gel electrophoresis step which allows students to visualize the DNA after performing the LAMP reaction. For this step, standard DNA Gel electrophoresis equipment will be required.