Sim Medium

To assist in the differentiation of enteric organisms the medium provides three tests - indole production, production of hydrogen sulfide by organisms that can reduce sulfur, and motility.

• Requires Kovac’s reagent for determination of indole production

To prepare media, add 30 g of SIM medium to a liter of distilled or de-ionized water. The media will not be sterile until autoclaved prior to use.