PCR Based Identification of Foodstuffs for Genetically Modified Organisms

For AP Biology Classes

• Testing for genetic engineering
• Time required: ~75 min plus 2 hours for PCR

Some foods contain raw materials from genetically modified organisms (GMO). Examples include tofu, corn flakes and corn meal. In this experiment, your students will extract DNA from food or plant material and perform PCR to determine if any GM indicator genes are present. Amplified DNA is separated and sized by agarose gel electrophoresis. (Requires 1 to 3 day shipping)

Some components require freezer storage on arrival.

Required, but not included: micropipettes to measure between 5 and 50 µl, tips, water bath, microcentrifuge, thermal cycler, electrophoresis apparatus, power supply, microwave or hot plate, UV transilluminator.

Delivery information: Kit includes: instructions, DNA extraction reagents, SYBR® Safe DNA Stain, PCR EdvoBeads™, microtubes, primers, DNA ladder, ultrapure water, wax beads, agarose, and electrophoresis buffer.