Exploring the Genetics of Taste: SNP Analysis of the PTC Gene using PCR
About this item
Let your students isolate, amplify and predict their ability to taste PTC paper.
- Students isolate and analyze their own DNA
- Total time (with stop-points) approximately 4 hours
- Perishable component refills available
The objective of this experiment is to identify the presence of the single nucleotide polymorphism (SNP) in an amplified segment of the PTC gene that links detection of the characteristic taste of PTC paper.
This is a set of five modules that starts with (I) extraction of DNA from buccal cells (II) amplifying the segment that contains the polymorphic nucleotide (III) digestion of the amplified fragment with the restriction enzyme that recognizes the SNP (IV) analysis by gel electrophoresis (V) tasting the PTC paper to confirm the results obtained.
Time Required: Extraction - 30 min; PCR Setup - 10 min; PCR - 2 hours; DNA Digest - 1 hr; Electrophoresis - 30 min.; Staining - 5 min.
Ordering information: Kit will ship immediately upon ordering. Please call to place your order if you would like to specify a future date for shipment.
Caution: Some components require Freezer storage upon receipt.
2 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
Let your students isolate, amplify and predict their ability to taste PTC paper.
- Students isolate and analyze their own DNA
- Total time (with stop-points) approximately 4 hours
- Perishable component refills available
The objective of this experiment is to identify the presence of the single nucleotide polymorphism (SNP) in an amplified segment of the PTC gene that links detection of the characteristic taste of PTC paper.
This is a set of five modules that starts with (I) extraction of DNA from buccal cells (II) amplifying the segment that contains the polymorphic nucleotide (III) digestion of the amplified fragment with the restriction enzyme that recognizes the SNP (IV) analysis by gel electrophoresis (V) tasting the PTC paper to confirm the results obtained.
Time Required: Extraction - 30 min; PCR Setup - 10 min; PCR - 2 hours; DNA Digest - 1 hr; Electrophoresis - 30 min.; Staining - 5 min.
Ordering information: Kit will ship immediately upon ordering. Please call to place your order if you would like to specify a future date for shipment.
Caution: Some components require Freezer storage upon receipt.
Documents
Product Family Options
Product Information
- No. of lab groupsAvailabilityPrice
- 470330-706Genetics of taste kitSupplier Number: 345Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 470330-708Kit refill (components A-G, PRO K)Supplier Number: 345-FBItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.