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Ward's® Transduction of E. coli Lab Activity

Avg. Ratings: 1.0 (1 Review)
Use Viruses to Deliver Genetic Material into Bacteria

  • Discuss applications for viral-induced genetic engineering
  • Materials for 8 lab groups
  • Time Required: ~120 minutes
Demonstrate transduction using a mutant E. coli, which is incapable of producing beta-galactosidase for lactose breakdown, and a lambda phage carrying the lac+ phenotype. Students will observe and differentiate between plaques and colonies to discover how this relates the lytic and lysogenic phases of transduction, and then will use their results to calculate the incidence of transduction for each plate. It includes enough materials for eight setups, a teacher’s guide, and student copymaster.

Ordering information: Coupon is included for redemption of perishable materials. Redeem by mail, phone, fax, or email. Activity includes 2 Tubes of tryptic soy broth; 2 Bottles; Luria agar; 1 Sleeve; Petri plates; 2 Cotton swabs; 2 Vials of N,N-dimethylformamide; 50 Disposable pipettes; 16 Capped vial; 20 Bacti-spreaders; 1 Tube of Sterile water; 1 Redemption coupon for: 1 Culture of E. coli host strain; 1 Tube of lambda phage gt11; 2 Vials of X-gal; and 2 Vials of IPTG.



   
 

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Item # Description No. of Lab Groups Unit Availability Price Quantity
470024-178 Transduction of E. coli Kit
8 Kit of 1 Retrieving Restricted
restricted This product is marked as restricted and can only be purchased by approved Shipping Accounts. To apply for a shipping account number, click here. If you need further assistance, call Customer Service at 800-962-2660 or email wardscs@vwr.com.

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REVIEWS (1)

Total Ratings: 1
Avg. Ratings: 1.0 out of 5

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Yes: 1 No: 1 
ScienceGuy Wallingford, PA
No, I didn't recommend
Transduction Lab Failure - This experiment HAS NOT WORKED the past 3 years that I have attempted (6 tries). They promise me every time that they have made "corrections" but still, no success. Save your districts money!!!
Reply
WARDSCI.com associate Published 05/29/2014
I am sorry this lab activity did not meet your standards.
 
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