Reverse Transcription PCR (RT-PCR): The Molecular Biology of HIV Replication

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470024-524EA 339 USD
Reverse Transcription PCR (RT-PCR): The Molecular Biology of HIV Replication
Educational Materials Biology Educational Materials PCR Learning Activities
Modern Application of Advanced Science

  • Advanced technique to explore transcription
  • Time required: ~90 min plus 2 hours for PCR
A specific mRNA is reverse transcribed to double-stranded DNA. This DNA product is then amplified by PCR. This reaction demonstrates the mode of replication of HIV, which contains reverse transcriptase. This experiment is the first introduction of a commercial RNA experiment for the classroom laboratory.

Kit includes: instructions, RNA Template, primer Mix, RT-PCR reaction beads, RNase-free water, DNA size ladder, agarose, electrophoresis buffer, InstaStain® Ethidium Bromide.

Required, but not included: thermal cycler, electrophoresis apparatus, power supply, microcentrifuge, automatic micropipette with tips, balance, microwave or hot plate, UV transilluminator, pipette pump or bulb, lab glassware, distilled or deionized water.

Packaging: Some components require freezer storage on arrival