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Ward's® Restriction Enzyme Digestion of DNA Lab Activity

E0303-WAR
This kit explores the effect restriction enzymes have on DNA.

  • Activities: Enzymatically Cleaving DNA and Analyzing on a Gel
  • Students Research Restriction Enzymes
  • Materials for Eight Lab Groups
  • Estimated Lab Time: 120 minutes
  • Teacher's and Student's Guide Included
Restriction enzymes, one of the primary tools in molecular biology, cut DNA molecules into precise fragments of a predictable size. Students can see the results by digesting lambda DNA with restriction enzymes Pvu II and EcoR I, running a gel, and then determining the base pair size of each fragment.

The kit includes a teacher’s guide, a student copymaster, and enough materials to run eight gels.

Ordering information: This kit contains bottle of 0.8% agarose, 200 mL; bottle of 5X TBE running buffer; concentrate, 500 mL; bottle of Ward’s QUIKView DNA stain concentrate, 60 mL; tube of Ethidium Bromide stain concentrate, 50 ?L; biohazard bag; microfuge tubes; gel staining trays; loading dye, 0.5 mL; coupon redeemable for: 80 ?L of Lambda DNA/Hind III; 3x80?L uncut Lambda DNA; 50?L of EcoR I restriction enzyme; 50?L of Pvu II restriction enzyme; 50 ?L of 10X EcoR I reaction buffer; and 50 ?L of 10X Pvu II reaction buffer. Materials needed but not provided are Electrophoresis chamber; Power supply; Incubator or hot water bath; micropipettes; metric ruler; permanent marker; calculator; gloves; goggles; apron UV Transilluminator; and UV goggles.



   
 

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Activity
Item # Description No. of Lab Groups Unit Availability Price Quantity
470024-472 Restriction Enzyme Digestion of DNA Kit
8 Kit of 1 Retrieving Restricted

Power Supply for Activity
Item # Description Unit Availability Price Quantity
470144-886 Ward’s Stackable Power Supply
Each Retrieving
restricted This product is marked as restricted and can only be purchased by approved Shipping Accounts. To apply for a shipping account number, click here. If you need further assistance, call Customer Service at 800-962-2660 or email wardscs@vwr.com.

SPECIFICATIONS

Output voltage range/increments (V) 2 - 300/1
Output current range/increments (mA) 4 - 500/1
Timer (h) 1 min. - 99 hr. 59 min.
Electrical 120 V

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