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Ward's® Regulation of Gene Expression: DNA Methylation Lab Activity

EPR1329
DNA methylation is used by bacteria, in nature, as a defense mechanism; it is also used medically to treat genetic diseases, such as Beta-thalassemia. To study DNA methylation, students methylate lambda DNA, then digest it with EcoR I and Hind III restriction enzymes to test its natural protection mechanism for DNA integrity, electrophoresing the samples to prove that the methylated DNA was protected. It comes with enough materials to run up to eight gels. The entire activity, including staining, can be completed in one 90-minute lab session, or can be divided into two 45-minute lab sessions.

  • Students Explore Epigenetic Effects of DNA Methylation
  • Materials for Eight Lab Groups
  • Time Required: ~90 minutes
Materials required, not included: An electrophoresis chamber, power supply, and 1-20 μL micropipettes.

Ordering information: Coupon is included for redemption of perishable materials. Redeem by mail, phone, fax, or email.



   
 

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Activity
Item # Unit Availability Price Quantity
470030-386 Regulation of Gene Expression Kit: DNA Methylation 8 Kit of 1 Retrieving Restricted

Power Supply for Activity
Item # Unit Availability Price Quantity
470016-052 AC/DC 6 V Power Supply EPR1329 Each Retrieving
restricted This product is marked as restricted and can only be purchased by approved Shipping Accounts. To apply for a shipping account number, click here. If you need further assistance, call Customer Service at 800-962-2660 or email wardscs@vwr.com.

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