Keep my session open?
Ending In 
The session is expired
Your session has expired. For your security, we have logged you out.
Would you like to log in again?

Ward's® Determining DNA Concentration and Purity Lab Activity

Analyze DNA Samples Using UV Spectrophotometry

  • Materials for 8 lab groups
  • Time Required: 40 min. (Part A) 1 hr.45 min. (Part B)
Techniques used to quantify DNA or to determine the purity of a DNA sample are important precursors to performing further analyses and validating results. In this activity, students will first learn to quantify unknown DNA samples by generating and interpreting a standard curve based on UV absorbance. They will then isolate DNA from their own cheek cells and determine the purity of the sample using a ratio of 260/280 nm wavelength. The kit includes enough materials for eight setups, a teacher’s guide, and student copymaster. A UV spectrophotometer, microcentrifuge, incubator, and waterbath, as well as quartz cuvettes and micropipettes, all required, are available separately.

Materials Included
100 mL 10X TE concentrate
100 mg Powdered DNA
375 Microcentrifuge tubes, 1.5 mL
5 Plastic bottles, 250 mL
20 mL 2X digestion/extraction buffer
10 mL 5M potassium acetate solution
30 mL 70% isopropanol
60 mL 95% ethanol
6 Sterile cotton swabs
1 Redemption coupon for: 1 300 µl tube Proteinase K, 20 mg/ mL, 1 300 µl tube RNase A, 4 mg/ mL

Materials Needed But Not Provided
Distilled or de-ionized water
UV-transparent cuvettes
UV spectrophotometer
1,000 µl micropipette and sterile tips
250 mL graduated cylinder
Ice bath
65°C water bath or incubator

Ordering information: Coupon is included for redemption of perishable materials. Redeem by mail, phone, fax, or email.



Item # Description No. of Lab Groups Unit Availability Price Quantity
470024-258 Determining DNA Concentration and Purity Kit
8 Kit of 1 Retrieving Restricted
restricted This product is marked as restricted and can only be purchased by approved Shipping Accounts. To apply for a shipping account number, click here. If you need further assistance, call Customer Service at 800-962-2660 or email


Need help?

  • Whatever you have to say, positive or negative, is important to us.
  • Providing feedback takes only seconds.
  • Your feedback will be made available to the public on the web. will publish your name as user.
You can use this name, or edit and save a nickname.
We recommend authors use their first and last initial.

Please confirm your profession

Example: Education

Please confirm your role

Example: Teacher

Please confirm your location

Example: New York, NY


Total Ratings: 0
Avg. Ratings: 0.0 out of 5

Sort all by:

Review & Compare Alternatives
We found alternative products that can save you up to  per item-unit.  To compare product details, select up to 3 alternatives below and click Compare Selected. To add items to your basket, enter a quantity and click Add to Basket.

Original Product:

Description Catalog Number Availability Unit Your Price Price Per Qty