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Ward's® Determining DNA Concentration and Purity Lab Activity


Analyze DNA Samples Using UV Spectrophotometry

  • Materials for 8 lab groups
  • Time Required: 40 min. (Part A) 1 hr.45 min. (Part B)
Techniques used to quantify DNA or to determine the purity of a DNA sample are important precursors to performing further analyses and validating results. In this activity, students will first learn to quantify unknown DNA samples by generating and interpreting a standard curve based on UV absorbance. They will then isolate DNA from their own cheek cells and determine the purity of the sample using a ratio of 260/280 nm wavelength. The kit includes enough materials for eight setups, a teacher’s guide, and student copymaster. A UV spectrophotometer, microcentrifuge, incubator, and waterbath, as well as quartz cuvettes and micropipettes, all required, are available separately.

Materials Included
100 mL 10X TE concentrate
100 mg Powdered DNA
375 Microcentrifuge tubes, 1.5 mL
5 Plastic bottles, 250 mL
20 mL 2X digestion/extraction buffer
10 mL 5M potassium acetate solution
30 mL 70% isopropanol
60 mL 95% ethanol
6 Sterile cotton swabs
1 Redemption coupon for: 1 300 µl tube Proteinase K, 20 mg/ mL, 1 300 µl tube RNase A, 4 mg/ mL

Materials Needed But Not Provided
Distilled or de-ionized water
UV-transparent cuvettes
UV spectrophotometer
1,000 µl micropipette and sterile tips
250 mL graduated cylinder
Microcentrifuge
Ice bath
65°C water bath or incubator

Ordering information: Coupon is included for redemption of perishable materials. Redeem by mail, phone, fax, or email.


   
 

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Item # Description No. of Lab Groups Unit Availability Price Quantity
366270 Determining DNA Concentration and Purity Kit
8 Kit of 1 Retrieving Restricted
restricted This product is marked as restricted and can only be purchased by approved Shipping Accounts. To apply for a shipping account number, click here. If you need further assistance, call Customer Service at 800-962-2660 or email wardscs@vwr.com.

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