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Biuret Reagent Solution

Avg. Ratings: 5.0 (2 Reviews)
BB0150-1L BB0150-100ML BB0150-500ML BB0145-50G
CAS Number: Mixture
Formula Weight: Mixture
Density: 1.0 g/mL
Boiling and Freezing Point: Approximately 100°C, Approximately 0°C
Synonyms: Biuret Solution, Biuret Reagent
Shelf Life: 12 Months

Note: The chemical specifications for some items on this product page may differ from the specifications listed above. For more information, consult the Safety Data Sheet or contact your Ward's representative.

Delivery information: This product is designed for educational and teaching laboratories, and no certificate of analysis is available.

Caution: This item is corrosive.



Item # Description Grade Size Chemical/Physical Specifications Unit Availability Price Quantity
470300-396 Biuret Reagent, Solution
Hazardous Product
Laboratory 100 mL Solution Each (100ml) Retrieving Restricted
470300-398 Biuret Reagent, Solution
Hazardous Product
Laboratory 500 mL Solution Each (500ml) Retrieving Restricted
470300-394 Biuret Reagent, Solution
Hazardous Product
Laboratory 1 L Solution Each (1l) Retrieving Restricted
470300-392 Biuret Powder
Laboratory 50 g Powder Each (50g) Retrieving Restricted
restricted This product is marked as restricted and can only be purchased by approved Shipping Accounts. To apply for a shipping account number, click here. If you need further assistance, call Customer Service at 800-962-2660 or email


This disposal method is published for your convenience. You MUST have checked with your federal, state, and local regulations before using this method and these methods are only applicable for small laboratory sized quantities.

Use a lab apron, chemical resistant gloves and goggles before following the below method to dispose of hazardous waste.

 1. The concentration of the base should be 1 M or less. Dilute it to this concentration if it is more concentrated. Use a large beaker and place it in a fume hood.
  a. When dissolving a base, heat may be evolved. Ice can be used to help control the heat.
  b. Work with a small amount of base at first, and then scale up.
 2. Slowly add dilute hydrochloric acid to the base until the mixture is neutralized to about a pH of 7.
 3. Pour the final solution down the drain with at least a 20-fold excess of water.

Solution Prep

To prepare Biuret's Reagent Solution, add 2.3 g of copper(II) sulfate pentahydrate to 225 ml of DI water. In a second beaker, add 775 ml of DI water. Place this beaker in an ice water bath. Next, add 308 g of sodium hydroxide to the 775 ml of DI water. Mix the sodium hydroxide solution with the copper sulfate solution. The solution should be blue.


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Total Ratings: 2
Avg. Ratings: 5.0 out of 5

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Was this review helpful? Yes No
Yes: 0 No: 0 user education / teacher / washington state
Yes, I recommend
Looking forward to starting our new bio-tech classes next semester
Reply associate Published 01/02/2018
Thank you!
Was this review helpful? Yes No
Yes: 0 No: 0 user Education / Teacher / Williams Lake, BC, CA
Yes, I recommend
This is a great reagent for detecting the protein content of samples. I used it for a vomit and diarrhea high school lab activity and it worked wonders!
Reply associate Published 06/14/2017
Thank you for sharing. We love your playful use of our product to engage your students in the classroom.
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