Using real-world forensic lab techniques, students learn which human DNA sequences are used for crime scene, missing person, mass disaster, and paternity investigations.
- Students Solve a Crime Using Basic Biotechnology
- Students Learn Real Forensic Techniques
- Teacher's and Student's Guides Provided
- Supplies and Techniques Used in Today's Most Advanced Biotech Labs
- Materials to Support 32 Students
Using polymerase chain reaction (PCR) and DNA electrophoresis, students determine the genotypes of five DNA samples and perform a simple statistical analysis on their own results to solve a forensic mystery. DNA profiling determines the exact genotype of a DNA sample and distinguishes one human being from another by identifying a DNA “bar code” that is unique to each individual. Within the variant areas of the genome exist unique polymorphic sequences called short tandem repeats (STRs) of uncertain biological function.
It is these DNA sequences that are used in forensic applications. The DNA samples contained in this introductory PCR kit are plasmids engineered to mimic the natural variations in human DNA that exist between one human being and another at a single STR locus. The lab requires two 45 minute lab sessions and includes materials for eight setups.
Ordering information: This kit includes DNA samples (crime scene, Suspect A, Suspect B, Suspect C, Suspect D), PCR master mix, primers, allele ladder (200 ?L), Orange G loading dye, PCR tubes, PCR tube adaptors, colored microcentrifuge tubes, foam floats, curriculum including a teacher’s guide, a student manual, and a graphic quick guide. Horizontal gel electrophoresis chambers, adjustable micropipets (2–20 ?L, 20–200 ?L), pipet tips, microcentrifuges, a thermal cycler, and power supplies are required but not provided.