Teach the concept of loop-mediated isothermal amplification (LAMP) at home, without the expensive equipment.
- Perform a simulated DNA amplification process
- Verify presence of LAMP-amplified DNA by color change
- Kit comes with lesson on LAMP and follow-up questions
- Teacher to aliquot samples for students in advance
In this activity, students play the role of the diagnostician in the following scenario. Patients arrive at the emergency room with fever and cough. A viral infection is suspected and must be confirmed or ruled-out from patient samples. Pre-amplified plasmid DNA is used to emulate the virus; no actual viral particles are used.
Kit includes enough for 24 sets of reagents to be aliquoted by the teacher into individual tubes for take-home by students. Each student will experience both a positive and negative reaction. Samples may be stored at room-temperature. Empty tubes for aliquoting not included.
DNA is pre-amplified, so the LAMP-step is simulated. Kits with actual LAMP reaction also available.