Features a variety of stimulating experiments to help students learn about the science of DNA and its application. The power of molecular cloning and restriction enzyme is remarkable.
Curriculum Module Includes: • Micropipetting Basics • Principles and Practice of Agarose Gel Electrophoresis (2) • Restriction Enzyme Cleavage of Plasmid and Lambda DNA • Size Determination of DNA Restriction Fragments (2) • Mapping of Restriction Sites on Plasmid DNA (2) • Mini-Prep Isolation of Plasmid DNA • Isolation of E.coli Chromosomal DNA • Cleavage of DNA With Restriction Enzymes (2) • Transformation of E. coli with GFP and BFP • Blue/White Cloning of a DNA Fragment and Assay of B-galactosidase (2) • Construction and Cloning of a DNA Recombinant (2) • Purification of the Restriction Enzyme Eco RI (2) • Cloning of a PCR Amplified Gene (2)
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