155222 Results for: "slides-and-cover-glasses&amp"

Supplier: Thermo Scientific Chemicals

Antimony(III) potassium oxitartrate trihydrate ACS

Supplier: Spectrum Chemical Mfg. Corp.

D-(+)-Camphor, also known as 2-Camphanone, is a terpinoid with a strong aromatic odor. This waxy, flammable, solid is used as an antimicrobial, a moth repellent and is readily absorbed through the skin producing a feeling of cooling similar to that of menthol. Ungraded products supplied by Spectrum are indicative of a grade suitable for general industrial use or research purposes and typically are not suitable for human consumption or therapeutic use. These materials may or may not have a Certificate of Analysis available.

Supplier: Aladdin Scientific

Cilengitide trifluoroacetate Cilengitide (EMD 121974, NSC 707544) is a potent integrin inhibitor for αvβ3 receptor and αvβ5 receptor with IC50 of 4.1 nM and 79 nM in cell-free assays, respectively; ~10-fold selectivity against gpIIbIIIa. Phase 2. Targetsαvβ3 receptor (Cell-free assay); αvβ5 receptor (Cell-free assay) 4.1 nM; 79 nMin vitro Cilengitide is a cyclized pentapeptide peptidomimetic designed to compete for the arginine-glycine-aspartic acid (RGD) peptide sequence that regulates integrin-ligand binding. Cilengitide selectively and potently blocks the ligation of theαvβ3 andαvβ5 integrins to provisional matrix proteins such as vitronectin, fibronectin, fibrinogen, von Willebrand factor, osteopontin, and others. Cilegitide inhibits angiogenesisin vitro. 10 μM Cilengitide completely inhibits attachment of BAE, BME and HUVE cells on vitronectin and fibronectin. Cilengitide inhibitsin vitro angiogenesis of BAE cells on three-dimensional collagen and fibrin gels pretreated with FGF-2(or VEGF-A) with IC50 of 15 μM and 8 μM, 4 μM and 3 μM, respectively. Cilengitide blocks proliferation and induces apoptosis of endothelial cells as well as differentiation of human endothelial precursor cells (EPCs). 50 μg/ml Cilengitide completely inhibits the proliferation of human microvascular endothelial cell line HMEC-1 and leads to apoptosis in ~30% cells. 1.0 μM Cilengitide treating for 9 days inhibits the proliferation of EPCs by nearly 40%. 1 μM Cilengitide inhibits the differentiation of EPCs by more than 80% at 14 days.

Supplier: Aladdin Scientific

CHIR-99021 (CT99021) HCl is hydrochloride of CHIR-99021, which is aGSK-3α/βinhibitor withIC50of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2. CHIR-99021 is a potent pharmacological activators of theWnt/beta-cateninsignaling pathway. CHIR-99021 significantly rescues light-inducedautophagyand augments GR, RORα and autophagy-related proteins.TargetsGSK-3β (Cell-free assay); GSK-3α (Cell-free assay) 6.7 nM; 10 nM In vitro CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ).

Supplier: Aladdin Scientific

AZD5582, a novel small-molecule IAP inhibitor, binds potently to the BIR3 domains ofcIAP1,cIAP2, andXIAPwithIC50values of 15, 21, and 15TargetscIAP1 (Cell-free assay); XIAP (Cell-free assay); cIAP2 (Cell-free assay) 15 nM; 15 nM; 21 nMIn vitroHuman pancreatic cancer cells display different sensitivities to the synthetic IAP antagonist, AZD5582. Treating human pancreatic cancer cells with AZD5582 differentially induces apoptosis, dependent on the expression of p-Akt and p-XIAP. It targets cIAP1 to induce TNF-α-induced apoptosis. AZD5582 induces a decrease of Mcl-1 protein, a member of the Bcl-2 family, but not that of Bcl-2 and Bcl-xL. HNSCC (head and neck squamous cell carcinoma) cell lines SCC25, Cal27, and FaDu show a dose-dependent cytotoxic effect after treatment with AZD5582.In vivoAfter AZD5582 treatment, tumor growth and weight decrease, whereas cleaved caspase 3 expression increases in Panc-1-derived xenograft model. When administered intravenously to MDA-MB-231 xenograft-bearing mice, AZD5582 results in cIAP1 degradation and caspase-3 cleavage within tumor cells and causes substantial tumor regressions following two weekly doses of 3.0 mg/kg. Following a modest 0.5 mg/kg intravenous bolus dose of AZD5582 in mice, unbound plasma levels remain above the concentrations at which apoptosis induction and cell death are observed in MDA-MB-231 cells over the course of several hours. Although cIAP1 degradation happens very quickly upon exposure to AZD5582 apoptosis induction (as measured by the amount of cleaved caspase-3) takes longer to reach a maximal effect.

Supplier: Aladdin Scientific

AZ304 is a synthetic inhibitor designed to interact with the ATP-binding site ofwild type and V600E mutant BRAFwith IC50 values of 79 nM and 38 nM, respectively. It also inhibitsCRAF, p38 and CSF1Rat sub 100 nM potencies.Targetsp38 (Cell-free assay); CSF1R (Cell-free assay); BRAF(V600E) (Cell-free assay); CRAF (Cell-free assay); WT BRAF (Cell-free assay) 6 nM; 35 nM; 38 nM; 68 nM; 79 nMin vitro AZ304 shows potent inhibitory activities to the kinase domains of wild type BRAF, V600E mutant BRAF and wild type CRAF with IC50 values of 79\u2009nM, 38\u2009nM and 68\u2009nM, respectively. AZ304 potently reduces ERK phosphorylation (p-ERK), with a mean EC50 of 65\u2009nM in the V600E mutant BRAF containing melanoma cell line A375 and an EC50 of 60\u2009nM in the wild type BRAF containing melanoma cell line SK-MEL-31. AZ304 markedly inhibits cell proliferation in mutant BRAF cancer cell lines, and effectively reduces cell growth in selected cell lines harbouring wild type BRAF/RAS or mutant RAS. The GI50 values ranged from 0.08-7.72\u2009μM in mutant BRAF cell lines, 0.43-11.7\u2009 μM in wild type BRAF/RAS cell lines, and 0.9-16.66\u2009μM in mutant RAS cell lines. AZ304 exhibits anti-proliferative effects on multiple cancer types, including melanoma, colorectal cancer, leukaemia, ovarian cancer, lung cancer, and pancreatic cancer, independently of BRAF genetic status. AZ304 retains inhibitory activity against both V600E mutant and wild type BRAF CRC cell lines in the presence of the EGFR ligand EGF.in vivoAZ304 monotherapy and its combination with Cetuximab have anti-tumour effects on RKO and Caco-2 tumour xenografts without obvious toxicity, independently of BRAF mutation status.Cell Research(from reference)Cell lines: cell lines containing wild type BRAF or V600E mutant BRAF (MC-F7, A549 and A375 cells) Concentration: 6.5 nM, 65 nM, 650 nM and 6.5 μM Incubation Time: 75 min.

Supplier: MP Biomedicals

Sodium phosphate is a reagent with very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Sodium phosphate occurs in several forms: monobasic (NaH2PO4), dibasic (Na2HPO4), and tribasic (Na3PO4). Most neutral sodium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.
Sodium phosphate monobasic, Monohydrate is used as sequestrant, emulsifier and buffer in foods; as mordant in dyeing; for weighting silk in tanning; in manufacture of enamels, ceramics, detergents, boiler compounds; as fireproofing agent; in soldering and brazing instead of borax; as reagent and buffer in analytical chemistry; cathartic; laxative. A table for preparation of 0.1 M sodium phosphate buffer at 25 °C using various proportions of sodium phosphate monobasic and sodium phosphate dibasic has been published. A study of the effect of freeze-thaw storage cycles on proteins in sodium phosphate and potassium phosphate buffer solutions has been reported. The effect of 5 mM sodium phosphate on the efficacy of electrospray ionization (ESI) ion mobility spectrometry (IMS) analysis has been evaluated. A protocol for the purification of pyrogen-free mouse IgG1 monoclonal antibodies which uses 10 mM sodium phosphate (pH 7.4) has been published. An ion-pairing HPLC method for the analysis of 5-aminosalicylic acid has been reported. A TLC method for separation of nucleotide sugars in the study of glycosyltransferase activity has been published.
Room Temperature, Desiccate

Supplier: Aladdin Scientific

Atuveciclib (BAY-1143572) is potent and highly selectivePTEFb/CDK9inhibitor withIC50values of 13 nM for CDK9/CycT and the ratio of IC50 values for CDK2/CDK9 is about 100. Outside the CDK family, It inhibits GSK3 kinase with IC50 values of 45 and 87 nM for GSK3α and GSK3β respectively.TargetsCDK9 (Cell-free assay); GSK-3α ; GSK3β 13 nM; 45 nM; 87 nM in vitro BAY\u20051143572 is a potent and highly selective CDK9 inhibitor (IC50 CDK9/CycT1: 13\u2005 nM, ratio of IC50 values CDK2/CDK9: 100). Outside the CDK family, submicromolar inhibitory activity was only recorded against GSK3 kinase (IC50 GSK3α: 45\u2005 nM, GSK3β: 87\u2005 nM). BAY\u20051143572 demonstrates antiproliferative activity against HeLa cells (IC50 = 920\u2005 nM) and MOLM-13 cells (IC50 = 310\u2005 nM). It also demonstrates improved Caco-2 permeability and a decreased efflux ratio (PappA→B: 35\u2005 nm/s, ER: 6) relative to lead compound BAY‐958 (PappA→B: 22\u2005 nm/s, ER: 15). in vivo In an in\u2005vivo pharmacokinetic study in rats, BAY\u20051143572 showed low blood clearance (CLb 1.1\u2005L/h/kg). The volumes of distribution (Vss) of BAY\u20051143572 is 1.0\u2005L/kg. BAY\u20051143572 shows significantly improved oral bioavailability of 54\u2009%. The blood/plasma ratios is about 1. It does not show significant inhibition of cytochrome P450 activity, with IC50 values >20\u2005 μM. The administration of BAY 1143572 in immunocompromized NOD/Shi-scid/IL-2Rγ (NOG) mice xenografted with patient-derived ATL cells greatly reduced the infiltration of ATL cells into organs, such as liver and bone marrow. Decreased human soluble IL2R levels in serum were also observed, which indicated a reduction of ATL tumor burden.

Supplier: Strem Chemicals Inc

CAS #: 6381-59-5. Size: 100g.

Supplier: IBI Scientific

The IBI rBAC Mini Total RNA kit is optimized for use with Gram(-) or Gram(+) bacteria

Supplier: Zymo Research

The YeaStar™ RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe.

Supplier: MP Biomedicals

Storage: -20°C, desiccate
This is an ultrapure NAD, chromatographically purified to remove trace inhibitors.
β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. It occurs in living cells primarily in the oxidized state. Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.
Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.

Supplier: Ambeed

L(+)-Histidine monohydrochloride monohydrate ≥98%

Supplier: Aladdin Scientific

Avitinib (AC0010) Avitinib (AC0010) is a pyrrolopyrimidine-based irreversible EGFR inhibitor that is mutation-selective with IC50 value of 0.18 nM against EGFR L858 R/T790 M double mutations, nearly 43-fold greater potency over wild-type EGFR (IC50 value, 7.68 nM). It has comparable anti-tumor activity and tolerated toxicity. TargetsJAK3 (Cell-free assay); EGFR L858 R/T790 M (Cell-free assay); BTK (Cell-free assay) 0.09 nM; 0.18 nM; 0.4 nMIn vitroAC0010 selectively inhibits EGFR active and T790 M mutations with up to 298-fold increase in potency compared to wild-type EGFR. AC0010 selectively inhibited mutant EGFR phosphorylation with IC50 values of 7.3 nM and 2.8 nM in NCI-H1975 and NIH/3 T3_TC32 T8 cells, about 115- and 298-fold more sensitive than that of the inhibition of wild type EGFR in A431. Immunoblotting analysis confirmed that AC0010 potently inhibited EGFR-Tyr1068 phosphorylation in NCI-H1975 cells, and the selectivity ratio is at 65-fold for NCI-H1975 cells versus A431 cells. In addition to inhibition of EGFR-Tyr1068 phosphorylation, AC0010 inhibited phosphorylation of the downstream targets Akt and ERK1/2, two important kinases involved in cancer cell proliferation and survival, in NCI-H1975 and HCC827 cells. The selectivity of AC0010 was also assessed by testing its activity against a panel of 349 kinases. At a concentration of 1 μM, AC0010 exhibited greater than 80% inhibition in 33 out of 349 unique kinase assays (9.5%). Kinase targets with greater than 80% inhibition include JAK3, BTK and 5 TEC family members. However, at the cellular level, the kinase inhibitory potency is much less than with the enzymatic assay. Much weaker inhibition was seen in BTK and JAK3 cellular assays with IC50 values of 59 nM and 360 nM.

Supplier: IBI Scientific

IBI gBAC Mini DNA Bacteria Kit is optimized for genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells

Supplier: Indofine Chemical Company

Rare Organics & BioChemicals 7048-04-6 250gm H-Cys-OH. HCI. H2O 175.6 Room temperature.

Supplier: MP Biomedicals

Cycloheximide is a glutarimide antibiotic derived from a microbial source. Cycloheximide is an antibiotic which is very active against many molds, yeasts, and phytopathogenic fungi. It exhibits somewhat lower activity against bacteria and certain fungi. Control of various molds and fungi in gelatin-based photographic emulsions, photoengraving glues, and other light-sensitive products is suggested.
Cycloheximide is used in plant research to study disease resistance and as an ethylene stimulant, useful in studies involving fruit and leaf production. It is also used in bacteriological media to isolate or count bacteria in the presence of yeast and molds; Used in protein synthesis in apoptosis; Gene expression; Glycogenolysis, gluconeogenesis and ureogenesis in isolated rat hepatocytes; Studies involving steroidogenesis; Used in plant regulation and as a quality control measure by the food and beverage industry.
Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors. Cycloheximide inhibits peptide synthesis in eukaryotic organisms but not in prokaryotes. Protein synthesis is blocked by the interaction of cycloheximide with the translocase enzyme. This interaction prohibits the translocation of messenger RNA on the cytosolic, 80S ribosomes without inhibiting organelle protein synthesis. Cycloheximide is also known to induce FAS/FAS Ligand apoptosis, and triggers apoptosis in HL-60 cells, T-cell hybridomas, Burkitt's lymphoma cells in addition to a variety of other cell types. Cycloheximide will also delay or inhibit apoptosis induced by other agents.

Supplier: Spectrum Chemical Mfg. Corp.

L-Cysteine Monohydrochloride, Monohydrate, FCC - The FCC grade meets the requirements of the Food Chemical Codex indicates and is suitable for all food, beverage and nutritional supplement applications. Spectrum Chemical offers over 300 Food grade chemical ingredients packaged in laboratory size bottles to production drum quantities and are manufactured, packaged and stored under current Good Manufacturing Practices (cGMP) per 21CFR part 211 in FDA registered and inspected facilities.

Supplier: MP Biomedicals

Potassium sodium tartrate tetrahydrate has been used in organic synthesis to break up emulsions in aqueous workups.

Supplier: MP Biomedicals

Arachidonic Acid is an essential fatty acid. Occurs in liver, brain, glandular organs, and depot fats of animals, in small amounts in human depot fats, and is a constituent of animal phosphatides.
Arachidonic Acid is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes. Arachidonic acid plays a key role in cellular regulation and is controlled through multiple interconnected pathways.
Arachidonic acid (AA) is an unsaturated ω6 fatty acid constituent of the phospholipids of cell membranes. Phospholipase A2 releases AA from the membrane phospholipids in response to inflammation. AA is subsequently metabolized to prostaglandins and thromboxanes by at least two cyclooxygenase (COX) isoforms, to leukotrienes and lipoxins by lipoxygenases, and to epoxyeicosatrienoic acids via cytochrome p450-catalyzed metabolism. AA and its metabolites play important roles in a variety of biological processes, including signal transduction, smooth muscle contraction, chemotaxis, cell proliferation and differentiation, and apoptosis. AA has been demonstrated to bind to the a subunit of G protein and inhibit the activity of Ras GTPase-activating proteins (GAPs). Cellular uptake of AA is energy dependent and involves protein-facilitated transport across the plasma membrane.
If ethanol is undesirable, arachidonic acid may be dissolved in acetonitrile, DMF, or DMSO. Simply evaporate the ethanol under a gentle stream of nitrogen (be certain not to evaporate the material to dryness) and redissolve the arachidonic acid in the solvent of choice.Just prior to use, make dilutions of the stock solution into aqueous buffer or isotonic saline to bring the arachidonic acid to the desired concentration. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiologic effects at low concentrations. A control using the solvent in the absence of the prostaglandin will address this potential variable. We do not recommend storing the aqueous solution for more than one day. It is difficult to obtain aqueous solutions of arachidonic acid directly. However, an organic solvent free solution of arachidonic acid can be prepared using concentrated basic buffers (pH > 8.0 and ionic strength not less than 0.1 M). Add 400 μL of cold buffer (0 °C) per mg of arachidonic acid and agitate vigorously and/or ultrasonicate.

Supplier: Ambeed

Sodium-L(+)-glutamate monohydrate 99%

Supplier: VWR International

The VWR® Nano Spectrophotometer LS is the affordable choice for laboratories needing to determine nucleic acid and protein concentrations and purity of samples.

Supplier: MP Biomedicals

L-Glutamic Acid is a non-essential amino acid for human development; referred to as an excitatory amino acid (EAA) due to its role in neurotransmission. Acts as a neurotransmitter at fast synapses; agonist at kainate, NMDA and quisqualate receptors. L-Glutamic acid is one of the two amino acids that contains a carboxylic acid group in its side chains.

Supplier: Novus Biologicals

Durable, nickel-plated magnetic clips are great for hanging notes, calendars, schedules, receipts, or photos on fridges, file cabinets, and other metal surfaces.

Supplier: Leica Microsystems

This configuration includes the Visoria M digital materials microscope that supports application performed in the metal, electronics, and polymer industries as well as for materials science labs.

Supplier: AOBChem USA

Adapt and evolve ideas with ease using EXPO® wet erase markers.

Supplier: Leica Microsystems

Streamline life science and clinical workflows with encoded functions and optimized light settings that ensure ergonomic comfort. This configuration includes the Visoria B laboratory microscope and is suited for Hematopathology or Clinical Microbiology applications.

Supplier: Leica Microsystems

The Visoria P polarization microscope uses polarized light to study the optical properties of materials and geological samples. You can streamline workflows with encoded functions, optimized light settings, and other microscope features.

Supplier: Leica Microsystems

The Visoria P polarization microscope uses polarized light to study the optical properties of materials and geological samples. You can streamline workflows with encoded functions, optimized light settings, and other microscope features.

Supplier: Leica Microsystems

Streamline life science and clinical workflows with encoded functions and optimized light settings that ensure ergonomic comfort. This configuration includes the Visoria B digital laboratory microscope and is suited for Histopathology or Cytopathology applications.