602 Results for: "reverse phase chromatography"

Supplier: Cytiva

SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high flow velocities and low back-pressure.

Supplier: Restek

Force C18 LC HPLC Columns are traditional end-capped C18 columns, ideal for general-purpose use in reversed-phase chromatography.

Supplier: MilliporeSigma

Ideal for reversed phase chromatography of basic compounds.

Supplier: Thermo Scientific

Achieve high-efficiency separations of hydrophobic, ionizable compounds with Thermo Scientific™ Dionex IonPac NS1 columns.

Supplier: AGILENT TECHNOLOGIES, INC (CSD)

Agilent offers a range of preparative columns for scale up of reverse phase chromatography, including C18, C8, and phenyl phases.

Supplier: Thermo Scientific

The Thermo Scientific™ IonPac™ NS2 is a unique silica based reversed-phase column designed for mobile-phase ion chromatography (MPIC) and compatibility with suppressed conductivity detectors.

Supplier: Thermo Scientific

Get all the advantages of reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) in one column with the Thermo Scientific™ Acclaim Mixed-Mode HILIC-1 column.

Supplier: Invitrogen

Pierce peptide retention time calibration mixture enables reversed phase liquid chromatography (LC) and mass spectrometry (LC-MS) users to optimize and confirm correct operation of their equipment.

Supplier: Cytiva

Source 15RPC ST 4.6/100 is a prepacked stainless steel column which provides fast, high-capacity preparative protein purification with reversed phase chromatography.

Supplier: Invitrogen

Thermo Scientific Pierce Marfey's Reagent (FDAA) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.

Supplier: Restek

Free-Turn architecture lets you change cartridges by hand without breaking inlet/outlet fluid connections, with no tools needed.

Supplier: Thermo Scientific

The Thermo Scientific™ DNAPac™ RP is a reversed-phase (RP) liquid chromatography column designed for analysis of oligonucleotides and double-stranded (ds) DNA/RNA fragments using LC/UV or LC/MS.

Supplier: SiliCycle

SiliaBond is SiliCycle's line of functionalized silica gels for use in organic synthesis.

Supplier: SiliCycle

SiliaBond is SiliCycle's line of functionalized silica gels for use in organic synthesis.

Supplier: Invitrogen

Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.Easy-to-use—resin provided in single-use spin column formatImproved protein identifications—protein identifications increased by ≥50% when compared to unfractionated samplesReproducible—elution profiles and fractional resolution vary by less than 20%Optimized—robust procedure for maximal protein identification and peptide recovery while minimizing fractional overlapCompatible—reagents validated with a variety of complex samples, including TMT™-labeled peptidesIn order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity and provides excellent orthogonality to low pH reversed-phase LC-MS gradients.The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a highpH fractionation protocol. In contrast to strong cation exchange (SCX) fractionation, the high-pH reversed-phase fractions do not require an additional desalting step before LC/MS analysis.The High pH Reversed-phase Peptide Fractionation Kit includes a high pH buffer (0.1% triethylamine) and twelve spin columns containing pH-resistant reversed-phase resin. Each reversed-phase fractionation spin column enables fractionation of 10–100 µg of peptide sample using a microcentrifuge.Native, phosphorylated, Tandem Mass TagTM (TMTTM)-labeled, and other complex peptide mixture samples can be fractionated using this kit. Combining the search results generated by the individual fractions helps improve protein sequence coverage and increases the number of identified proteins relative to unfractionated samples.Applications:Reducing sample complexity to identify targets of interestConducting systems biology studiesReducing sample complexity to improve quantitation studies

Supplier: MP Biomedicals

Sodium acetate is used as a buffer in the buffering range of pH 3.6-5.6. It is also used in the purification and precipitation of nucleic acids, protein crystallization, staining of gels in protein gel electrophoresis, HPLC, mordant dyeing, DNA microarray studies of E. coli response, investigation of protein unfolding during reverse phase chromatography.

Supplier: MP Biomedicals

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature

Supplier: Avantor

These text mixtures are used for reversed and normal-phase chromatograms for a 250×4.6 mm Int.Ø columns.

Supplier: Avantor

Vydac MS columns are a later development from grace for the reversed phase HPLC separation of biomolecules.

Supplier: Avantor

Vydac MS columns are a later development from Grace for the reversed phase HPLC separation of biomolecules.

Supplier: Avantor

Avantor® ACE® CN-ES combines CN polar selectivity with enhanced hydrophobicity, enabling the benefits of both interactions to be fully exploited. Extra phase stability and increased column lifetime is provided by an extended alkyl chain spacer between the silica surface and cyano group. Avantor® ACE® CN-ES is suitable for both reversed phase and normal phase separations.

Supplier: Hichrom

Avantor® ACE® UltraCore C18-Amide are high performing solid-core (core shell) particle columns designed for a range of reversed-phase chromatographic applications. Additional mechanisms of interaction such as H-bonding give an alternative selectivity for moderately polar analytes.

Supplier: Avantor

Vydac MS columns are a later development from Grace for the reversed phase HPLC separation of biomolecules.

Supplier: Avantor

Vydac MS columns are a later development from Grace for the reversed phase HPLC separation of biomolecules.

Supplier: Thermo Scientific

For unique selectivity of aromatic compounds and superior chromatographic performance, choose Thermo Scientific™ Acclaim Phenyl-1 reversed-phase columns.

Supplier: Avantor

These C8 columns offer high performance in order to tackle the most challenging reversed-phase applications. The use of ultra pure silica, advanced bonding technology, superior column specification and comprehensive batch validation all contribute to the columns' excellent reproducibility.

Supplier: Thermo Scientific

Resolve polar and non-polar compounds in a single run with Thermo Scientific™ Acclaim PolarAdvantage II (PA2) reversed-phase columns.

Supplier: MicroSolv Technology Corp

Using proprietary bonding technology, columns are bonded directly to the nonpolar TYPE-C™ silica surface with two separate points of attachment, allowing them to operate in three different domains: reverse phase, normal phase and aqueous normal phase.

Supplier: Thermo Scientific

For separations of hydrophobic, structurally related isomers, Thermo Scientific™ Acclaim C30 reversed-phase columns provide superior results.

Supplier: Avantor

Avantor® ACE® AQ is an ultra-inert phase designed for the reversed-phase separation of very polar compounds with up to 100% aqueous eluent. ACE AQ is ideal for use with fast gradients due to its rapid re-equilibration properties and resistance to retention loss.