1103 Results for: "protein purification system"

Supplier: Abnova

Human PHGDH full-length ORF recombinant protein with GST-tag at N-terminal.

Supplier: G-Biosciences

The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research

Supplier: Cytiva

HiPrep Phenyl FF (High Sub) 16/10, are prepacked Phenyl Sepharose 6 Fast Flow columns ready to use for hydrophobic interaction chromatography (HIC).

Supplier: Cytiva

HiTrap SP XL are strong cation exchangers prepacked with SP Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography.

Supplier: MP Biomedicals

β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Supplier: Abnova

Human PPP1R14B full-length ORF recombinant protein with GST-tag at N-terminal.

Supplier: Cytiva

HiPrep Sephacryl S-300 HR columns are designed for routine size exclusion chromatography of proteins, such as antibodies and serum proteins.

Supplier: Thermo Fisher Scientific

Purifies DNA, RNA, proteins, or cells in strips of five tubes.

Supplier: FUJIFILM IRVINE SCIENTIFIC WAKO CHE

This antibody specifically recognizes the PA tag (GVAMPGAEDDVV) and is ideal for detecting PA-tagged proteins in various applications such as Western blotting, immunoprecipitation, and immunocytochemistry.

Supplier: Lucigen

Protect your precious RNA with this high quality, effective RNase inhibitor.

Supplier: Prosci

SSX2IP belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). It may connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin.

Supplier: MilliporeSigma

Aprotinin is a competitive and reversible inhibitor of esterases and proteases.

Supplier: Cytiva

HiScreen Capto S columns are prepacked with Capto S ion exchange chromatography medium, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

Supplier: Enzo Life Sciences

Inhibitor of PP1 and PP2A

Supplier: MP Biomedicals

Lysing Matrix Z is used for breaking tough plant and animal samples.

Supplier: Cloud-Clone

This is a H6 conjugated OVA, General species is sequencing from HHHHHH with 90 to 100% purity. Lyophilized from PBS, pH 7.4, containing 0.01% SKL, 5% Trehalose with 0.2 mg/ml.

Supplier: Rockland Immunochemical

Rabbit Anti-Maltose Binding Protein (MBP) Epitope Tag Biotin Conjugated Antibody

Supplier: FUJIFILM IRVINE SCIENTIFIC WAKO CHE

This antibody is Anti PA tag, Monoclonal Antibody conjugated horse radish peroxidase.

Supplier: EXPRESSION SYSTEMS LLC

BestBac Linearized Baculovirus DNA backbone has been engineered to deliver highly efficient recombination with all polyhedron gene locus based transfer vectors.

Supplier: Buchi

Standard Silica Flash Cartridges are used for optimum chromatographic performance.

Supplier: Cytiva

HiScreen™ Capto™ DEAE column is a weak anion exchanger with a bed height of 10 cm making it an excellent tool for method optimization, parameter screening, robustness testing, and convenient scale-up.

Supplier: MP Biomedicals

Green caps fit EMPTY FASTPREP® tubes and are used in fastprep systems, to hold sample for homogenization.

Supplier: Invitrogen

M-PER™ Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

Supplier: Cytiva

HiTrap HIC Selection Kit is designed for rapid screening for the most appropriate hydrophobic interaction chromatography (HIC) resin and experimental conditions.

Supplier: Lonza

Serum-free media provide better control over the physiological condition of cultures while allowing simplified purification and downstream processing protocols

Supplier: Cytiva

HiPrep Q FF is a strong anion exchanger prepacked with Q Sepharose Fast Flow, ready-to-use for fast, preparative separations of proteins and other biomolecules using ion exchange (IEX) chromatography.

Supplier: Invitrogen

The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimized protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.MS-compatible—reagents directly compatible with in-solution peptide digestion, enriched samples contain minimal detergent residuals detected using LC-MSSensitive—procedure successfully enriches low abundance proteins (low ng range)Low background—binding, wash, and elution buffers optimized to minimize enrichment of background proteinsRobust—procedure and reagents have been robustly tested with numerous targets to ensure consistent enrichment of low abundance proteins (ng range) with at least 2 peptides identified per proteinThe Pierce MS-Compatible Magnetic IP Kit (Protein A/G) uses high-quality Pierce Protein A/G Magnetic Beads to provide wider flexibility of antibody capture than either Protein A or G alone

Supplier: G-Biosciences

Concentrated, pH-stable buffer component widely used in the preparation of phosphate buffer systems.

Supplier: Cytiva

HiPrep DEAE FF 16/10 is a weak anion exchanger prepacked with DEAE Sepharose Fast Flow, ready-to-use for fast, preparative separations of proteins and other biomolecules using ion exchange (IEX) chromatography.

Supplier: Genscript

THE™ Anti-GST Monoclonal antibody (Mouse) recognizes GST localized at the C-terminal, N-terminal and internal region ofGST-tagged fusion proteins.