1105 Results for: "protein purification system"

Supplier: Cytiva

Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability

Supplier: Invitrogen

The Thermo Scientific Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

Supplier: OZ Biosciences

Incomplete Freund’s Adjuvant (IFA) is a water-in-oil emulsion that is similar to the Complete Freund’s Adjuvant (CFA) without addition of heat-killed mycobacteria (Mycobacterum butyricum).

Supplier: Cytiva

SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.

Supplier: Cytiva

Capto™ L is a bioprocessing chromatography resin for the capture of antibodies and antibody fragments. It combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein, L ligand, which has a strong affinity to the variable region of antibody kappa light chains. It is suitable for the capture of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

Supplier: Invitrogen

Thermo Scientific Pierce CDI-Activated Agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilization of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.

Supplier: MilliporeSigma

Streptavidin agarose for column or batch isolation of biotinylated molecules.

Supplier: MilliporeSigma

D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.

Supplier: Abcam

Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell.

Supplier: Cytiva

The illustra™ triplePrep Kit is designed for the rapid, simultaneous extraction and isolation of high yield genomic DNA (gDNA), total RNA, and total denatured proteins from undivided animal tissues and mammalian cells. The streamlined workflow reduces the overall number of steps, enabling the preparation of all three analytes in less than one hour.

Supplier: Invitrogen

The Pierce™ protease and phosphatase inhibitor tablets contains protease and/or phosphatase inhibitors to provide broad spectrum complete protections to proteins from degradations.

Supplier: Invitrogen

Thermo Scientific AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.

Supplier: Invitrogen

Pierce™ HA-Tag IP/Co-IP kit provides the affinity resin, positive control and other reagents necessary to perform immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) reactions with an HA-tagged bait protein.

Supplier: Cytiva

Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.

Supplier: Cytiva

Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can, therefore, be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

Supplier: Cytiva

EAH Sepharose™ 4B allows for the stable carbodiimide-based coupling of carboxyl groups to Sepharose® 4B via an 11-atom hydrophilic spacer arm that permits extremely stable coupling.

Supplier: Invitrogen

Thermo Scientific Pierce Fab Micro Preparation Kit uses immobilized papain protease to digest human or mouse IgG antibodies to make separate Fab and Fc fragments and subsequently to purify the Fab using Protein A agarose.

Supplier: G-Biosciences

G-Biosciences' Tri-Xtract™ is a convenient, ready-to-use reagent designed for the isolation of total RNA that is free from protein and DNA contamination

Supplier: IMPACT PROTEOMICS, LLC MS

The ProMTag Multiomics DNA, RNA, Protein Sample Preparation kit produces high-quality DNA, RNA, and peptide samples from the same starting sample.

Supplier: TCI America

Protein A is a type I membrane protein produced by several strains of Staphylococcus aureus. It has high-affinity binding sites that enable the purification of IgG obtained from various species such as humans, rabbit, mouse, and bovine. Protein A Agarose is prepared using a covalent coupling method that maintains the stability and binding characteristics of the product.
By using our resin, human IgGs can be eluted under milder conditions (such as at pH 4.0) as compared to elution by conventional methods.
Additional information is available on our website.

Supplier: Abcam

ChIP Kit (Transcription factors, ChIP-seq) (ab270813) is a highly validated solution for robust transcription factor and other non-histone proteins ChIP-seq results and contains everything you need for start-to-finish ChIP prior to Next-Generation Sequencing.

Supplier: Cytiva

DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

Supplier: Cytiva

SP Sepharose Fast Flow is a strong cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Supplier: Cytiva

DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.

Supplier: G-Biosciences

G-Sep™ DEAE Agarose Fast Flow (FF) resin is a weak anion exchanger composed of highly cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups.

Supplier: Cytiva

Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Supplier: Invitrogen

The Pierce™ Direct IP Kit uses an activated resin to covalently immobilize IP antibodies (any species or class) on agarose beads without the aid of Protein A/G, enabling immunoprecipitation without antibody interference.

Supplier: Cytiva

rProtein A Sepharose™ 4 Fast Flow is a well established antibody affinity medium is designed for the purification of monoclonal and polyclonal antibodies based on Sepharose™ Fast Flow platform. The recombinant protein A is produced in E.coli and engineered for an oriented coupling to giving a matrix with enhanced binding capacity. The epoxy-based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A and provides excellent purification in one step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ Fast Flow ideal for purification of monoclonal antibodies at both laboratory and process scale.

Supplier: Bio-Rad

Chelex® 100 Chelating Resin is a weak acidic cation exchange resin that has high selectivity, approximately 5,000:1, for copper, iron, and other heavy metal ions over monovalent cations. Chelex 100 Resin is made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.

Supplier: Abcam

Alexa Fluor® 647 antibody labeling/conjugation kit, Lightning-Link® requires < 20 minutes with 30 seconds of hands-on time. 100% antibody recovery. - Cited in over 40 publications - 15-minute incubation time - Alexa Fluor® 647 labeled antibody is ready for use in applications like WB, ELISA, and IHC, with no need for further purification - Compatible with most standard antibody formulations - Scalable; use the same conjugation method from 10µg-100mg of antibody - Use Lightning-Link® conjugation with over 45 labels, including Alexa Fluor®, DyLight®, tandem dyes, enzymes, oligonucleotides, and gold nanoparticles