1103 Results for: "protein purification system"

Supplier: Anaspec

Protein A-HiLyte™ Fluor 555 Conjugate can be used as a universal reagent to detect primary antibodies in IHC from many species including rabbit, human, some mouse IgG isotypes, and others. Fluorescence (orange) Excitation/Emission wavelength: 553 nm/568 nm.
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed using a recombinant bacterial expression system. Its apparent molecular weight is approximately 30,000 Da compared to 42,000 Da for the native Protein A that is found in Staphylococcus aureus.
Protein A conjugates are widely used as labeled reagents for a variety of experiments including immunoprecipitation, antibody detection and purification and assay development

Supplier: Anaspec

Protein A-HiLyte™ Fluor 750 Conjugate can be used as a universal reagent to detect primary antibodies in Western Immunoblot from many species including rabbit, human, some mouse IgG isotypes, and others. Fluorescence (near-infrared) Excitation/Emission wavelength: 754 nm/778 nm.
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed using a recombinant bacterial expression system. Its apparent molecular weight is approximately 30,000 Da compared to 42,000 Da for the native Protein A that is found in Staphylococcus aureus.
Protein A conjugates are widely used as labeled reagents for a variety of experiments including immunoprecipitation, antibody detection and purification and assay development

Supplier: Anaspec

Protein A-HiLyte™ Fluor 647 Conjugate can be used as a universal reagent to detect primary antibodies in IHC from many species including rabbit, human, some mouse IgG isotypes, and others. Fluorescence (near-infrared) Excitation/Emission wavelength: 649 nm/674 nm.
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed using a recombinant bacterial expression system. Its apparent molecular weight is approximately 30,000 Da compared to 42,000 Da for the native Protein A that is found in Staphylococcus aureus.
Protein A conjugates are widely used as labeled reagents for a variety of experiments including immunoprecipitation, antibody detection and purification and assay development

Supplier: Proteintech

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (Maltose binding protein) TAG in some expression systems.

Supplier: MP Biomedicals

Peroxidase-conjugated goat IgG fraction to human IgG Fc is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to human IgG Fc and buffer salts.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Peroxidase-conjugated goat IgG fraction to human IgG Fc is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from inherent antibodies of host animals.

Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.

The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with human IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis.

Supplier: Abcam

Magnetic Antibody Purification System (ab270532) is a convenient method to purify antibodies at a small scale with high recoveries.

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Silica based media used for the purification of proteins based on differential salt induced hydrophobicity.

Supplier: Invitrogen

Melon™ Gel Monoclonal IgG Purification Kit is a high-yield, mild purification system for monoclonal antibodies from ascites or hybridoma cell culture supernatant, or for large scale antibody purification from serum.

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

The J.T.Baker® BAKERBOND® PROchievA™ protein A resin offers superior dynamic binding capacity with excellent alkaline stability linked to Avantor's proprietary ligand.

Supplier: Abcam

Efficient mouse antibody purification from tissue culture supernatants.

Supplier: Zymo Research

A method for fast His-tagged protein purification.

Supplier: Cube Biotech

PureCube Co-NTA magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.

Supplier: Cube Biotech

PureCube Ni-IDA MagBeads were developed by Cube Biotech for His-tag protein purification.

Supplier: Abcam

Protein G Agarose is useful for affinity purification of IgG from many mammalian species.

Supplier: Cube Biotech

The HisCube MagBead protein purification MINI kit is designed to meet the demands of your laboratory for efficient and high-quality small-scale His-tag protein purifications.

Supplier: AAT Bioquest

ReadiLink™ xtra rapid antibody labeling kits require essentially only two simple mixing steps without a column purification needed.

Supplier: Cube Biotech

The protein purification with the PureCube Glutathione MagBeads and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.

Supplier: Cube Biotech

PureCube Zn-NTA magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.

Supplier: G-Biosciences

The Calmodulin Resin Kit is for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1 mg calmodulin/ml resin.

Supplier: Abcam

TCS Antibody Purification Kit - Nanoparticles (ab270554) is designed for the purification of IgG fractions from tissue culture supernatants.

Supplier: Cube Biotech

PureCube Ti-NTA MagBeads/magnetic beads were developed for the enrichment of phosphorylated proteins. They can also be used for the purification of His-tagged proteins as a secondary function.

Supplier: Abcam

Rat TCS Antibody Purification Kit (ab270562) is designed for the purification of rat IgG fractions from tissue culture supernatants.

Supplier: AAT Bioquest

ReadiLink™ xtra rapid antibody labeling kits require essentially only 2 simple mixing steps without a column purification needed.

Supplier: Abcam

TCS Antibody Purification Kit (ab109207) has been designed for tissue culture supernatant purification, and can be used to purify 10-50 mL in each purification.

Supplier: 3M Healthcare

Single-stage chromatographic purification for recombinant protein therapeutic manufacturing.

Supplier: Abcam

Mouse Antibody Purification Kit - Nanoparticles (ab204910) removes contaminants often found in commercial antibodies.

Supplier: G-Biosciences

Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin.

Supplier: Abcam

Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species.

Supplier: AAT Bioquest

ReadiLink™ xtra rapid antibody labeling kits require essentially only two simple mixing steps without a column purification needed.

Supplier: Cytiva

Biacore maintenance kits are convenient kits for proper instrument maintenance.