4186 Results for: "polymerase"

Supplier: New England Biolabs (NEB)

Q5 High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.

Supplier: Bioss

This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq, Jul 2008]

Supplier: Bioss

DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases (1). In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches (2). These exonucleases include the family of DNA polymerases (3). DNA pol , _, ∂, and e are involved in DNA replication and repair (4). DNA pol ∂ and DNA pol e are multisubunit enzymes, with DNA pol ∂ consisting of two subunits p125, which interacts with the sliding DNA clamp protein PCNA, and p50 (5). The nuclear-encoded DNA pol © is the only DNA polymerase required for the replication of the mitochondrial DNA (6). DNA pol is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis (7). DNA pol œ is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks (8).

Supplier: New England Biolabs (NEB)

Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5´ → 3´ polymerase activity, but lacks 5´ →3´ exonuclease activity.

Supplier: Novus Biologicals

DNA polymerase sigma Lysate (Adult Normal)

Supplier: Novus Biologicals

DNA Polymerase iota Lysate (Adult Normal)

Supplier: Novus Biologicals

The LOC285697 TAF11 RNA polymerase II Antibody from Novus Biologicals is a mouse polyclonal antibody to LOC285697 TAF11 RNA polymerase II. This antibody reacts with human. The LOC285697 TAF11 RNA polymerase II Antibody has been validated for the following applications: Western Blot, ELISA.

Supplier: Bulldog Bio

TaqDog PCR enzymes can be used in many types of experiments that require a robust and active Taq polymerase. It’s highly stable, and able to be diluted, making it perfect for labs that are looking for a reliable and cost effective PCR enzyme.

Supplier: Stemcell Technologies

Polymerase, buffers, and dNTPs for high-fidelity PCR amplification.

Supplier: Intact Genomics

i7™ Hhot start high-fidelity DNA polymerase 2X master mix is ready to use premix which contains hot start high-fidelity DNA polymerase, dNTPs,MgCl₂, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7™ hot start high-fidelity DNA polymerase 2X master mix has the high-fidelity, sensitivity and processivity with an error rate ~2.8×102 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes.

Supplier: Novus Biologicals

The DNA polymerase delta p50 Antibody from Novus Biologicals is a rabbit polyclonal antibody to DNA polymerase delta p50. This antibody reacts with human. The DNA polymerase delta p50 Antibody has been validated for the following applications: Western Blot.

Supplier: New England Biolabs (NEB)

Bst 2.0 DNA Polymerase is an in silico designed homologue of Bacillus stearo thermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment)

Supplier: Novus Biologicals

The DNA Polymerase epsilon subunit 3 Antibody from Novus Biologicals is a rabbit polyclonal antibody to DNA Polymerase epsilon subunit 3. This antibody reacts with human, mouse. The DNA Polymerase epsilon subunit 3 Antibody has been validated for the following applications: Western Blot, Immunoprecipitation, Immunohistochemistry-Paraffin.

Supplier: Novus Biologicals

DNA polymerase eta Lysate (Adult Normal)

Supplier: Novus Biologicals

DNA polymerase mu Lysate (Adult Normal)

Supplier: Bioss

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.

Supplier: Novus Biologicals

DNA Polymerase Kappa Lysate (Adult Normal)

Supplier: Abcam

Human DNA polymerase eta peptide is a Synthetic blocking peptide. >90% purity and suitable for BL.

Supplier: Enzo Life Sciences

Increased sensitivity standard PCR for a broader range of samples, with enhanced speed, yield, and specificity.

Supplier: Alkali Scientific

Unlock precise amplification with Radiant™ Hot start Taq polymerase.

Supplier: Agilent Technologies

Cloned Pfu DNA Polymerase for high fidelity and difficult, GC-Rich PCR.

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant T7 RNA Polymerase. The specific IgG was purified by T7 RNA Polymerase affinity chromatography.

Supplier: EMPIRICAL BIOSCIENCE

Phu-50 High Fidelity Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Phu DNA polymerase but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5 - 3' direction but does not possess a 5 - 3' exonuclease replacement activity. Its inherent 3 - 5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Phu-50 Polymerase-generated PCR fragments are blunt.

Supplier: New England Biolabs (NEB)

Deep VentR (exo-) DNA Polymerase has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity associated with Deep Vent DNA Polymerase

Supplier: Antibodies.com

Rabbit polyclonal antibody to DNA polymerase I for WB and IP with samples derived from Escherichia coli.

Supplier: Bon Opus Biosciences

Bon Opus Biosciences offers quality recombinant proteins products covering a broad collection of cytokines, enzymes, diagnostic and detection reagents, and other protein-related products

Supplier: Bioss

RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Supplier: Novus Biologicals

The RNA polymerase I termination factor Antibody from Novus Biologicals is a rabbit polyclonal antibody to RNA polymerase I termination factor. This antibody reacts with human. The RNA polymerase I termination factor Antibody has been validated for the following applications: Western Blot.

Supplier: New England Biolabs (NEB)

Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor.

Supplier: Antibodies.com

Rabbit polyclonal antibody to DNA Polymerase lambda for WB and ELISA with samples derived from Human, Mouse and Rat.