600 Results for: "pcr enzymes and reagents"

Supplier: Corning

The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilizes a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimized buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Supplier: Promega Corporation

HaloTag Cloning Starter Kit: designed to use Flexi Cloning System to clone protein coding sequences into mammalian HaloTag expression vectors; clone and transfer gene sequences into C- or N-terminus of HaloTag Flexi CMV Deletion Vectors.

Supplier: G-Biosciences

G­Biosciences' Toothpick™ systems allow for the rapid screening of bacterial colonies by restriction enzyme digestion analysis or polymerase chain reaction (PCR), with our Plasmid Screening Toothpick™ and Toothpick™­PCR, respectively.

Supplier: Stemcell Technologies

For extraction of DNA from agarose gels, or clean-up of PCR products and restriction enzyme digestions.

Supplier: Cytiva

The ExoProStar™ S PCR and Sequence Reaction Clean-Up Kit updates existing enzymatic PCR clean up technology with Exonuclease I and Shrimp Alkaline Phosphatase to remove unincorporated primers and dNTPs. illustra ExoProStar™ S improves digestion efficiency with no degradation of the target PCR product. Sample remain completely intact and ready for immediate use in manual or automated processes. For maximum flexibility the enzymes are supplied in two separate tubes.

Supplier: Cytiva

The illustra™ plasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification. Produces high quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing.

Supplier: G-Biosciences

G-Biosciences' GET™ CLEAN DNA kit uses high binding affinity GET™ Spin Columns to remove exess salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparation of 100 base pairs to >20kb

Supplier: Cytiva

illustra™ tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high quality genomic DNA from a variety of animal tissues and mammalian cell cultures. The kit can be used for several downstream applications including restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR.

Supplier: Zymo Research

The RNA Clean & Concentrator™ (RCC™) kits facilitate the efficient removal of RNA polymerases, ligases, and RNA modifying enzymes.

Supplier: Abcam

Mitochondrial DNA isolation kit provides efficient tools for isolating mitochondrial DNA (mtDNA) from a variety of cells and tissues, offering high yield and purity, free from genomic DNA contamination. - Cited in over 65 publications - Isolates mtDNA with no genomic DNA contamination - Suitable for enzyme manipulations, Southern blotting, PCR analysis, and amplifications - Simple and fast procedure for mtDNA isolation from cells or tissue - One kit is sufficient for 50 samples using adherent or suspension cells, and for 25 samples using tissue.

Supplier: Zymo Research

The YeaStar™ RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe.

Supplier: Omega Bio-Tek

Magnetic separation device-A consists of 24 powerful magnetic rods that fit between the wells of commercially-available 96-well microplates

Supplier: Zymo Research

Quick (2 minute) recovery of ultra-pure DNA and RNA oligonucleotides.

Supplier: Omega Bio-Tek

Isolate DNA from plant or fungal samples using spin columns.

Supplier: Corning

AxyPrep™ Mag plasmid purification Kit utilizes a magnetic bead technology for high throughput purification of plasmid DNA from E.coli cells. The AxyPrep™ Mag kit can also be used with fosmid and BAC vector-based constructs ranging from 5 to 150 Kb. The system consists of steps of pelleting cells, resuspension, lysis, neutralization, DNA binding, ethanol washing and plasmid elution. The protocol is automation-compatible.

Supplier: G-Biosciences

G-Biosciences' GET™ Plasmid Miniprep kit isolates high quality plasmid DNA from 1-5 mL E

Supplier: G-Biosciences

G-Biosciences offers a wide selection of genomic isolation kits that purify high quality genomic DNA from a variety of sources and for a wide array of applications. G-Biosciences' XIT™ DNA kits produce protein-free, high quality DNA through the principle of cell lysis, protein digestion and precipitation, and genomic DNA purification. No chloroform or phenol extraction is required. High quality DNA can be isolated from sample types including: animal tissues, cells, whole blood, bacteria, Buccal cells, plant tissues, mouse tail, yeast, and FFPE tissue. G-Biosciences' XIT™ DNA kit procedures remove contaminants and enzyme inhibitors, allowing the purified DNA to be ready for immediate use for all downstream analyses. The purified DNA from G-Biosciences' XIT™ DNA kits have a A₂₆₀/A₂₈₀ ratio, between 1.7 and 1.9 (with the exception of the Buccal cells kit with a ratio between 1.8 and 2.0), and are up to 200 kb in size.

Supplier: New England Biolabs (NEB)

The NEB Golden Gate Assembly Kit (BsmBI-v2) contains an optimized mix of BsmBI-v2 and T4 DNA Ligase. BsmBI-v2 has been engineered by NEB and outperforms BsmBI in Golden Gate Assemblies.

Supplier: Omega Bio-Tek

Gel purification of DNA is a common technique used to isolate specific DNA fragments following agarose gel electrophoresis

Supplier: Omega Bio-Tek

The E.Z.N.A.® Viral RNA Kit is designed for the isolation of viral RNA from cell-free fluids such as plasma, serum, urine, and cell culture supernatant

Supplier: Omega Bio-Tek

The Endo-Free Plasmid Midi Kits provide an efficient method for the large-scale isolation of endotoxin-free plasmid DNA

Supplier: Omega Bio-Tek

The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30µg of high-quality plasmid DNA from 1–5mL bacterial cultures in 30 minutes or less

Supplier: Omega Bio-Tek

The Endo-Free Plasmid Maxi Kits provide an efficient method for the large-scale isolation of endotoxin-free plasmid DNA

Supplier: Omega Bio-Tek

The E.Z.N.A.® Plasmid Maxi Kit is a maxi-scale system designed to deliver high-quality plasmid DNA using the spin column format, without costly accessories

Supplier: Biochain Institute Inc

This dNTP reagent combines the highest quality with the most competitive price on the market.

Supplier: New England Biolabs (NEB)

The LunaScript Multiplex One-Step RT-PCR Kit offers a streamlined protocol for cDNA synthesis and PCR amplification in a single reaction.

Supplier: New England Biolabs (NEB)

WarmStart RTx Reverse Transcriptase is a unique in silico designed RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits RTx activity below 40°C

Supplier: Promega Corporation

M-MLV RT is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb).

Supplier: Promega Corporation

The AccessQuick™ RT-PCR System is an easy and convenient master mix for one-tube RT-PCR.

Supplier: New England Biolabs (NEB)

Bacteriophage T3 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T3 phage promoters