600 Results for: "pcr enzymes and reagents"

Supplier: Biotium

EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-color tracking or pre-mixed with low or high ROX.

Supplier: Bio-Rad

iScript™ RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis, RNA stabilization, and removal of genomic DNA for sensitive qPCR without traditional RNA sample preparation.

Supplier: New England Biolabs (NEB)

The Control LAMP Primer Mix (rActin) targets actin RNA at an exon-exon junction. It is a LAMP primer set (F3, B3, FIP, BIP, LF, LB) that amplifies rActin to ensure the absence of inhibition from human nucleic acid templates in a LAMP reaction.

Supplier: Quantabio

qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5’-hydrolysis probes on real-time PCR systems that do not require a passive reference dye. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. qScript XLT One-Step RT-qPCR ToughMix contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: Intact Genomics

The new improved IG® Recombinase Polymerase Amplification version 2 kit (IG® RPA v2) helps researchers who seek the advantages of isothermal DNA amplification to achieve faster, more discernible results.

Supplier: New England Biolabs (NEB)

The Luna Probe One-Step RT-qPCR Mix with UDG (No ROX) is supplied at a 4X concentration and enables higher amounts of sample input, which is relevant for applications where RNA present in low abundance is of interest, such as pathogen detection.

Supplier: Intact Genomics

igScript™ RT-qPCR kit requires minimal handling during reaction setup and offers consistent and robust RT-qPCR reactions.

Supplier: Biotium

EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

Supplier: New England Biolabs (NEB)

In the Luna Probe One-Step RT-qPCR Kit (No ROX), Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition.

Supplier: EMPIRICAL BIOSCIENCE

The qPCR MasterMix is supplied as a 2X product and is a ready to use HotStart qPCR MasterMix with EvaGreen® dye, FlashTaq DNA polymerase, dCTP, dGTP, dATP, dTTP, and MgCl2. EvaGreen® is a dsDNA-binding dye. EvaGreen® possesses similar absorption and emission spectra as SYBR® Green I.

Supplier: Intact Genomics

Intact Genomics propriety Ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site.

Supplier: New England Biolabs (NEB)

The phi29-XT RCA DNA amplification kit includes everything needed for sensitive and robust rolling circle amplification (RCA), an isothermal amplification approach to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence.

Supplier: New England Biolabs (NEB)

The Luna® Cell Ready One-Step RT-qPCR Kit provides all the necessary components for direct, dye-based RNA detection and quantitation, bypassing the need for RNA extraction and purification.

Supplier: New England Biolabs (NEB)

The Luna® Cell Ready Probe One-Step RT-qPCR Kit provides all the necessary components for direct, probe-based RNA detection and quantitation, bypassing the need for RNA extraction and purification.

Supplier: New England Biolabs (NEB)

The Luna® Probe one-step RT-qPCR 4X mix with UDG is designed for real-time detection of target RNA sequences using hydrolysis probes.

Supplier: New England Biolabs (NEB)

Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets.

Supplier: Intact Genomics

Propriety ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR products to any linearized expression vector at any site.

Supplier: Promega Corporation

DNA-dependent RNA polymerase for in vitro transcription.

Supplier: Promega Corporation

DNA-dependent RNA polymerase for in vitro transcription.

Supplier: Enzo Life Sciences

Complete kit to digest and label DNA for CGH+SNP arrays.

Supplier: Promega Corporation

DNA-dependent RNA polymerase for in vitro transcription.

Supplier: New England Biolabs (NEB)

Immobilized T4 DNA Ligase (IM-T4 DNA Ligase) is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg per ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, enzyme can be removed using a magnet thereby avoiding heat inactivation, and can be re-used.

Supplier: Promega Corporation

DNA-dependent RNA polymerase for in vitro transcription.

Supplier: Lucigen

Thermostable ligase for intramolecular ligation (i.e. circularization) of ssDNA templates

Supplier: Promega Corporation

DNA-dependent RNA polymerase for in vitro transcription.

Supplier: Promega Corporation

DNA-dependent RNA polymerase for in vitro transcription.

Supplier: Promega Corporation

DNA Polymerase I catalyzes the template-directed polymerization of nucleotides into duplex DNA in a 5'-->3' direction. Recombinant.

Supplier: Promega Corporation

High-fidelity polymerase useful for mutagenesis reactions, 3'-overhang removal and 5'-overhang fill-in reactions.

Supplier: Corning

The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilizes a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimized buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Supplier: Promega Corporation

A DNA-dependent DNA polymerase that lacks the 5'-->3' exonuclease activity of intact E. coli DNA Polymerase I but retains its 5'-->3' polymerase, 3'-->5' exonuclease and strand displacement activities.