3736 Results for: "nucleic acid "

Supplier: Zymo Research

The DNA/RNA Shield™ fecal collection tube is powered by the DNA/RNA shield sample stabilization reagent, which takes a microbial snapshot of a sample, inactivates viruses, and makes samples safe and transport ready. Samples are stable at ambient temperature and can be frozen for longer-term storage.

Supplier: Invitrogen

Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

Supplier: MP Biomedicals

β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Supplier: MP Biomedicals

DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.

DTT has been used:

Supplier: Molecular Devices

The GenePix® 4300A and GenePix® 4400A Scanners from Molecular Devices offer maximum imaging quality with optimal resolution in highly configurable platforms.

Supplier: Beckman Coulter

The Avanti™ J-15 series of benchtop centrifuges (refrigerated or ventilated) leverage the ultra harmonic technology, designed to protect your sample and increase workflow time efficiencies.

Supplier: Cytiva

The key to reproducible electrophoresis results is temperature control. Gels cooled on their two faces produce slanted bands appearing as broad or double bands in stained gels. SE 600 Ruby ensures uniform heat in gels because, the gels are immersed in the lower buffer for uniform heat transfer and a standard magnetic stirrer circulates buffer around the vertically suspended heat exchanger to maintain homogeneous lower buffer temperature. The ergonomic SE 600 Ruby provides two built-in side handles, which facilitates carrying and handling. Run up to four gels (up to 112 samples) in a single run. Divider plates convert single-gel sandwiches into two-gel club sandwiches, which seal and fit into the casting stand like single gels. If gels longer than 16-cm are required, Cytiva offers the SE 660, which runs gels up to 24 cm. Except for gel lengt, the SE 660 has identical features to those of SE 600 Ruby.

Supplier: MP Biomedicals

DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Useful for stabilizing sulfhydryl-containing enzymes.

Supplier: Beckman Coulter

The Avanti™ J-15 series of benchtop centrifuges (refrigerated or ventilated) leverage the ultra harmonic technology, designed to protect your sample and increase workflow time efficiencies.

Supplier: Biotium

PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

Supplier: MP Biomedicals

Storage: Room Temperature
2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its diminished vapor pressure, its odour, while unpleasant, is less objectionable than related thiols.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also it reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Supplier: Genevac

miVac Centrifugal Evaporator Packages can be configured out of the box for maximum convenience.

Supplier: 3M Healthcare

3M™ Encapsulated Production Systems can be pivoted to the horizontal position for ergonomic loading and unloading at waist height and to the vertical position for filtration. This means operators do not have to lift capsules above their heads and risk exposure to fluid spills when handling used capsules and drastically reduce the time it takes to change out filter cartridges.

Supplier: Thermo Fisher Scientific

Everylab Centrifuge packages come with the range of Finnpipette® F2 pipettors, containing an F-Stand, F2 Pen, F2 Brochure, reagent reservoir demo pack, Good laboratory pipetting guide, an assortment of compatible Finntip flex tips and a pack of microcentrifuge tubes. Finntip flextips are the most advanced traditional tips, offering very low attachment and ejection forces. The Good laboratory pipetting guide contains essential pipetting information, from choosing the right type to detailed decontamination information. Simply choose between the Everylab Centrifuge Refrigerated package or the Everylab Centrifuge Ventilated package.

Supplier: REVVITY HEALTH SCIENCES, INC.

The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. The VICTOR Nivo is a high-performance multimode microplate reader that can be equipped with all major detection technologies: Absorbance, Luminescence, Fluorescence, Time-Resolved Fluorescence, TR-FRET, Fluorescence Polarization, and Alpha Technology.

Supplier: REVVITY HEALTH SCIENCES, INC.

Oligo(dT) coated M-PVA magnetic beads.