521 Results for: "histology reagents"

Supplier: Epredia

PrintMate biopsy cassettes are designed for ease of use and print clarity.

Supplier: Epredia

PrintMate embedding cassettes are designed for ease of use and print clarity.

Supplier: MP Biomedicals

Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxicity and proliferation assays.
Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.

Supplier: DRG International

An enzyme immunoassay for the detection of IgM antibodies to Helicobacter pylori in serum.

Supplier: Epredia

PrintMate slotted cassettes are designed for ease of use and print clarity.

Supplier: Thermo Scientific Chemicals

MDL: MFCD00151093 Notes: Transition interval: pH 1.2 (red) to pH 2.8 (yellow); pH 8.0 (yellow) to pH 9.2 (blue)

Supplier: Thermo Scientific Chemicals

Congo red indicator

Supplier: Thermo Scientific Chemicals

Biological stain for distinguishing coli and aerogene bacteria

Supplier: Thermo Scientific Chemicals

Transition interval: pH 4.6 (yellow) to pH 6.0 (red)

Supplier: Thermo Scientific Chemicals

Propidium iodide, 1mg/ml aqueous soln.

Supplier: Thermo Scientific Chemicals

MDL: MFCD00148898 Notes: Transition interval: pH 3.8 (yellow) to pH 5.4 (blue)

Supplier: Thermo Scientific Chemicals

Tetrazolium derivative useful in cancer research

Supplier: Thermo Scientific Chemicals

MDL: MFCD00011750 Beilstein Registry No.: 4077708

Supplier: Thermo Scientific Chemicals

Used in measurement of cell proliferation. Produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells

Supplier: Thermo Scientific Chemicals

Fluorescein disodium salt hydrate is used in dye, ink pigment, in cosmetic products, to detect corneal epithelial defects, and also used as a fluorescent tracer to study blood-brain barrier.

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: Thermo Scientific Chemicals

Fuchsin (acid)

Supplier: MilliporeSigma

A lipophilic, water-soluble styrylpyridinium red fluoroprobe that is useful for staining active recycling synaptic vesicles.

Supplier: Adipogen

Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 6-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Spectral data: lambdaex 496 nm| lambdaem 516 nm (Buffer pH 9.0).

Supplier: Electron Microscopy Sciences

Price’s Giemsa Stain is used for hematologic elements. Stain results - Malarial Parasites: Blue, Tissue Nuclei: Blue, Bacteria: Dark Blue, Malarial Pigment: Black, Schistosonic egg shells: Blue, Collagen, etc.: Pale Pink, Erthrocytes: Pink Rose..

Supplier: Thermo Scientific Chemicals

Used for dehydrogenase determination

Supplier: Enzo Life Sciences

Cell permeable DNA stain that can be used for a wide range of applications

Supplier: AVIDITY SCIENCE

Provides laboratory grade, Type 2 water in two convenient production rates of 20 or 40 liters per hour.

Supplier: MP Biomedicals

Congo red is the sodium salt of 3,3'-([1,1'-biphenyl]-4,4'-diyl)bis(4-aminonaphthalene-1-sulfonic acid). It is a secondary diazo dye. It is absorption indicator for halide and thiocyanate determinations.

Supplier: Cytiva

Trypan Blue solution, 0,4%, is routinely used as a cell stain to assess cell viability using the dye exclusion test.

Supplier: Thermo Scientific Chemicals

MDL: MFCD00148896 Notes: Transition interval: pH 5.2 (yellow-green) to pH 6.8 (purple)

Supplier: Electron Microscopy Sciences

Price’s Giemsa Stain is used for hematologic elements. Stain results - Malarial Parasites: Blue, Tissue Nuclei: Blue, Bacteria: Dark Blue, Malarial Pigment: Black, Schistosonic egg shells: Blue, Collagen, etc.: Pale Pink, Erthrocytes: Pink Rose..

Supplier: MP Biomedicals

Phenol red is an acid-base indicator. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride.
Phenol Red is used as a pH indicator in cell culture applications. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH of 8.2 and above. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates.
Store at Room Temperature (15-30 °C)

Supplier: Biosensis

Fluoro-Jade stain is a fluorochrome derived from fluorescein, and is commonly used in neuroscience disciplines to label degenerating neurons in ex vivo tissue of the central nervous system.

Supplier: Thermo Scientific Chemicals

Used in dye exclusion studies in cell culture.