225 Results for: "crispr"

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DepleteX® Mitochondrial DNA Depletion Kit

Supplier: REVVITY HEALTH SCIENCES, INC.

The DepleteX® mitochondrial DNA depletion kit employs a straightforward CRISPR-based method to selectively remove unwanted human molecules derived from mitochondrial nucleic acid before sequencing.

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Genomic DNA Extraction

Supplier: TRIBIOSCIENCE, INC. MS

Fast DNA extraction kit provides a simple and rapid method to isolate the genomic DNA from tissues or cells for PCR amplification genotype identification CRISPR mutation detection and library screening.

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Cas9 Nuclease (from Streptococcus pyogenes)

Supplier: Intact Genomics

Cas9 nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing.

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CRISPRclean® Plus Total RNA Prep

Supplier: REVVITY HEALTH SCIENCES, INC.

The CRISPRclean® stranded total RNA prep with rRNA depletion (HMR + pan bacteria) is a total RNA library prep leveraging a CRISPR-powered workflow facilitating the detection of lower expressing and biologically relevant transcripts from complex samples.

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CRISPRclean® Stranded Total RNA Prep

Supplier: REVVITY HEALTH SCIENCES, INC.

The CRISPRclean® stranded total RNA prep with rRNA depletion (HMR) is a total RNA library prep leveraging a CRISPR-powered workflow facilitating the detection of lower expressing and biologically relevant transcripts from complex samples.

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Trifluorothymidine >98% Thymidylate synthase inhibitor; Anti cancer agent

Supplier: G-Biosciences

An analog of thymidine which inhibits thymidylate synthase possesses antiviral and anticancer activity.1,2 After phosphorylation by thymidine kinase, it is incorporated into DNA where it induces DNA-damage and interferes with repair enzymes.3 Enhances frame shift insertion and deletion in CRISPR genome editing in pluripotent stem cells.

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PEmax Enzyme, Intact Genomics

Supplier: Intact Genomics

PEmax enzyme is the purified recombinant Streptococcus pyogenes Cas9 nickase mutant (H840A)-Murine Moloney Leukemia Virus-Reverse Transcriptase (MMLV-RT) fusion protein3-4 containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR prime-editing3-4. The physical purity of this enzyme is ≥85% as assessed by SDS-PAGE with Coomassie® blue staining.

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Recombinant Cas9 Streptococcus pyogenes

Supplier: EMPIRICAL BIOSCIENCE

Cas9 is an RNA-guided endonuclease that is naturally expressed by Streptococcus pyogenes. Catalytically active ribonucleoprotein (RNP) complexes are produced by incubating Cas9 with a sequence specific CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), which together activate the endonuclease and guide it to the ~20-nucleotide protospacer sequence in target dsDNA. Alternatively, Cas9 can be complexed with a synthetic guide RNA (sgRNA) that encodes both the crRNA and tracrRNA in a single RNA strand.

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Nocodazole >98% Microtubule inhibitor

Supplier: G-Biosciences

An antimitotic agent that disrupts microtubules by binding to β tubulin and thereby inhibiting microtubule dynamics, disruption of mitotic spindle function, and fragmentation of the Golgi complex.1,2 Arrests cell cycle at G2/M phase. Stimulates the intrinsic GTPase activity of tubulin.3 Nocodazole activates the JNK/SAPK signaling pathway and induces apoptosis in a variety of cell lines.4 Increases Cas9-mediated editing frequencies5 and increased CRISPR-mediated HDR DNA repair6. Cell permeable.

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NU7441 >98% DNA-PK inhibitor

Supplier: G-Biosciences

Potent (IC50=13 nM) and selective ATP-competitive inhibitor of DNA-dependent protein kinase (DNA-PK).1,2 It increased the toxicity of ionizing radiation and etoposide on SW620 human colon cancer lines via impeding DNA double-strand break repair.3 NU7441 also increased the radio/chemosensitivity of various other cancer cell lines.3-6 Reduces the frequency of NHEJ repair while enhancing the rate of HDR repair following CRISPR/Cas9-mediated gene editing.

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AZD7762 >98% Checkpoint kinase 1/2 inhibitor

Supplier: G-Biosciences

Potent and selective inhibitor of checkpoint kinases 1 and 2 (IC50 = 5 nM for both).1 It abrogates DNA damage-induced S and G2 checkpoints and enhances the efficacy of DNA damaging agents such as gemcitabine and irinotecan. AZD7762 also enhanced the radiation sensitivity of p53-mutant tumor cell lines.2,3 AZD7762 was able to overcome imatinib resistance in CML cells.4 AZD7762 has also been reported to be a potent inhibitor of MEKK2 (MAP3K2) – IC50 = 20 nM.5 It has also recently been shown to inhibit antigen-stimulated degranulation from RBL-2H3 (IC50 = 28 nM) and BMMCs (IC50 = 99 nM) as well as suppressing degranulation of LAD2 human mast cells (IC50 = 50 nM) via Syk suppression through inactivation of Lyn and Fyn kinases.6 Enhances CRISPR-Cpf1-mediated precise genome editing.