971 Results for: "animal sera"

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgG2a covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG2a. This antibody was tested by Dot Blot and/or solid phase adsorbed for minimal cross-reactivity with mouse IgM, IgG1, IgG2b, IgG3, IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled IgG covalently linked to agarose. Based on ELISA, this antibody reacts with the heavy and light chains of goat IgG and with sheep IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rabbit, rat, chicken, guinea pig, hamster, swine, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using goat IgG covalently linked to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains of goat IgG and sheep IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rabbit, rat, chicken, guinea pig, hamster, swine, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

The antibody was isolated from antisera by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, the antibody reacts with the heavy and light chains on mouse IgG and with the light chains of mouse IgM and IgA. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, guinea pig, horse, human, rabbit, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgG1 covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG1. This antibody was tested by Dot Blot and/or solid phase adsorbed for minimal cross-reactivity with mouse IgM, IgG2a, IgG2b, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled IgG covalently linked to agarose. Based on ELISA, this antibody reacts with the heavy and light chains of goat IgG and with sheep IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rabbit, rat, chicken, guinea pig, hamster, swine, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgG2b covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG2b. This antibody was tested by Dot Blot and/or solid phase adsorbed for minimal cross-reactivity with mouse IgM, IgG1, IgG2a, IgG3, IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

The antibody was isolated from antisera by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, the antibody reacts with the heavy and light chains on rabbit IgG and with the light chains common to most rabbit immunoglobulins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

The antibody was isolated from antisera by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, the antibody reacts with the heavy and light chains on rabbit IgG and with the light chains common to most rabbit immunoglobulins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled rabbit IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled rabbit IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with goat, horse, human, rabbit, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with goat, horse, human, rabbit, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

The antibody was isolated by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains on rabbit IgG and with the light chains common to most rabbit immunoglobulins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins, but the antibody may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

The antibody was isolated by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains on rabbit IgG and with the light chains common to most rabbit immunoglobulins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins, but the antibody may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

The antibody was isolated from antisera by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains on mouse IgG and with the light chains of mouse IgM and IgA. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, guinea pig, horse, human, rabbit, and sheep serum proteins, but the antibody may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

The antibody was isolated from antisera by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains on mouse IgG and with the light chains of mouse IgM and IgA. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, guinea pig, horse, human, rabbit, and sheep serum proteins, but the antibody may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: MP Biomedicals

Peroxidase-conjugated goat IgG fraction to rabbit IgG (whole molecule) is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. Anti-Human IgG is developed in goat using IgG from pooled normal human serum as the immunogen. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins.

Specificity for human IgG is determined by immunoelectrophoresis (IEP) versus purified human IgA, IgG, IgM, Bence Jones kappa, and Bence Jones lambda myeloma proteins. Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation in the gamma region.

Peroxidase-conjugated goat IgG fraction to rabbit IgG (whole molecule) product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals.

Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.

The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with rabbit IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is predominantly goat IgG; no trace of albumin is detected.

Supplier: MP Biomedicals

Peroxidase-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) is horseradish peroxidase (HRP)-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) and buffer salts. Antiserum is produced in goat using purified rabbit IgG as the immunogen. Antibody is isolated from goat anti-rabbit IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to rabbit IgG. The antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross reactivity in tissue or cell preparations.

Specificity of the Anti-Rabbit IgG-Peroxidase is determined by immunoelectrophoresis (IEP) using normal rabbit serum and rabbit IgG. The conjugate shows no reaction with human IgG by IEP. Identity and purity of the antibody is established by immunoelectrophoresis prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.

Peroxidase-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. No preservative is added.

The total protein is measured using the Biuret procedure with bovine albumin as standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with rabbit IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is goat IgG; no trace of albumin is detected.

Supplier: MP Biomedicals

Product is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified antibody to mouse IgG (whole molecule) and buffer salts.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Anti-Mouse IgG (whole molecule)-FITC antibody reacts with normal mouse serum and all mouse IgGs.

Fluorescein-Conjugated Goat Affinity Purified Antibody to mouse IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of these affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).

Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v), adjusted to standard titer, filtered through a 0.22 μm filter, and vialed.

The total protein is measured by absorbance (A280 nm); F/P ratio is calculated using absorbance at 491.5 nm. Antibody titer of the affinity purified antibody is standardized by equivalence-point precipitation. The affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to non-immunoglobulin serum proteins is not present.

Supplier: MP Biomedicals

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to mouse IgG (whole molecule) and buffer salts. Anti-Mouse IgG is developed in goat using purified mouse IgG as the immunogen. Affinity isolation removes essentially all goat serum proteins, including immunoglobulins which do not specifically bind to mouse IgG.

This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals).

Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added.

The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with mouse IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is predominantly goat IgG; no trace of albumin is detected.

Supplier: MP Biomedicals

Fluorescein-conjugated Goat IgG fraction to Rabbit IgG is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts.

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Rabbit IgG (whole molecule)-FITC antibody reacts with rabbit serum and rabbit IgG.

Fluorescein-conjugated Goat IgG fraction to Rabbit IgG is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).

Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.

The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The IgG fraction is tested for purity and specificity at 40 mg/mL using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected.