971 Results for: "animal sera"

Supplier: Li-Cor

The antibody was isolated from antisera by affinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on rabbit IgG and with the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

The antibody was isolated from antisera by affinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on goat IgG and with the light chains common to most goat immunoglobulins. The antibody also reacts with sheep IgG. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum proteins, but the antibody may cross-react with immunoglobulins from other species. The conjugate was specifically tested and qualified for Western blot applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

The antibody was isolated from antisera by affinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on goat IgG and with the light chains common to most goat immunoglobulins. The antibody also reacts with sheep IgG. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum proteins, but the antibody may cross-react with immunoglobulins from other species. The conjugate was specifically tested and qualified for Western blot applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Proteintech

PON2(Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. .

Supplier: Aladdin Scientific

Product DescriptionDGAT-1 inhibitor A 922500 (A-922500) reduces serum triglyceride levels from baseline at all doses tested; however, this is only statistically significant at the 3 mg/kg dose, which lowers serum triglycerides by 53%. Similarly, the 3 mg/kg dose of A 922500 significantly reduces serum FFA concentrations by 55% and total cholesterol by 25%. DGAT-1 inhibition has no significant effect on body weight at any dose tested. Although A 922500 dpes not significantly affect LDL-cholesterol or HDL-cholesterol individually, the serum LDL/HDL-cholesterol ratio is significantly improved by A 922500 at 0.3 and 3 mg/kg. Similar to the dyslipidemic hamster, treatment with 3 mg/kg A 922500 significantly reduces serum triglyceride concentrations (39%). FFA levels significantly increase over the 14-day period in vehicle-treated animals. This increase is inhibited in a dose-dependent manner by A 922500 such that FFA concentrations are 32% lower after 14 days of treatment with the DGAT-1 inhibitor at 3 mg/kg, compared with the vehicle group (p < 0.05). HDL-cholesterol is significantly increased from baseline levels by A 922500 at 0.3 and 3 mg/kg; however, this is only significantly increased compared with vehicle at the 3 mg/kg dose. Body weight significantly increases over the 2-week period in vehicle-treated rats, and this is not affected by A 922500. LDL-cholesterol is significantly reduced in the vehicle treated group. DGAT-1 inhibition does not further reduce LDL-cholesterol and has no effect on total cholesterol.Product ApplicationA922500 has been used in the inhibition of diacylglycerol acyltransferase (DGAT) in epithelial human breast cancer cell line (MDA-MB-231), digitonin-permeabilized human cancer cells, human myotubes and macrophages.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule guinea pig IgG as well as the light chains common to other guinea pig immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Aladdin Scientific

Product Introductionrecombinant, expressed in rice, suitable for cell culture, low endotoxin;It is a kind of recombinant human albumin (RHA) without animal origin. It is designed for cell culture applications and is a powerful medium supplement with superior performance to human albumin, bovine serum albumin and recombinant human albumin from other sources.It brings stable and consistent performance rather than variables related to hydrolysates and extracts. It is an ideal medium for Chinese hamster ovary (CHO), hybridoma, Vero, stem cell and primary cell culture medium.The recombinant variant is recommended for absorption, transport, metabolism and excretion (ADME) pharmacology; cell culture; drug delivery; and cell cryopreservation.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled IgG covalently linked to agarose beads. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG, and with the light chains common to other guinea pig immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule guinea pig IgG as well as the light chains common to other guinea pig immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Anaspec

SensoLyte® Anti-PLP (139 - 151) IgG Quantitative ELISA Kit (Mouse) is optimized to detect mouse anti-PLP (139-151) IgG. This kit is useful to researchers who wish to determine the amount of anti-PLP (139-151) antibody present in biological samples, and can help provide information on the role it plays in the development and treatment of EAE, an animal model for MS pathogenesis. Wells are pre-coated with PLP (139-151) peptide and pre-blocked with BSA. The amount of anti-PLP (139-151) IgG in mouse serum or cerebrospinal fluid is quantified using ELISA (Abs=450 nm). Ample materials and reagents are provided to perform 96 assays.

Supplier: Anaspec

SensoLyte® Anti-PLP (178-191) IgG Quantitative ELISA Kit (Mouse) is optimized to detect mouse anti-PLP (178-191) IgG. This kit is useful to researchers who wish to determine the amount of anti-PLP (178-191) antibody present in biological samples, and can help provide information on the role it plays in the development and treatment of EAE, an animal model for MS pathogenesis. Wells are pre-coated with PLP (178-191) peptide and pre-blocked with BSA. The amount of anti-PLP (178-191) IgG in mouse serum or cerebrospinal fluid is quantified using ELISA (Abs=450 nm). Ample materials and reagents are provided to perform 96 assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using human IgG covalently linked to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains of human IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with bovine, horse, and mouse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: MP Biomedicals

Choline chloride is an organic compound and a quaternary ammonium salt. It is a weak acid. Choline chloride is the salt of the naturally occurring choline, the pre-stage of the neurotransmitter acetylcholine, which is important for mnemonic and thought-processes. Choline occurs naturally in fungi, hop and kingcups and as integral part of lecithin. Choline chloride is a common food additive in animal husbandry
The enzymatic activities of butyrylcholinesterase (BChE) and paraoxonase 1 (PON1), two serum enzymes synthesized by the liver and related with inflammation, were decreased in a sepsis animal model injected with LPS. Choline chloride administered intravenously at 20 mg/kg body weight prevents the LPS-mediated decreases in the activities of these two enzymes.
Choline chloride is used as a source of methyl groups for methyl-transfer reactions.
Physical Appearance: Hard, White Crystalline Powder
pH: Neutral to Litmus
Vapor Pressure: 10 hPa at 20 °C (Lit.)
Partition Coefficient: n octanol/water (logKow): -3.77 at 25 °C (lit.)
Solubility: Soluble in water: (10% aq soln) Clear, Colorless Solution

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using human IgG covalently linked to agarose beads. Based on ELISA, the antibody reacts with the heavy and light chains of human IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with bovine, horse, and mouse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using human IgG covalently linked to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains of human IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with bovine, horse, and mouse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose. Based on ELISA, FLISA, and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody was tested by Dot Blot and/or solid phase adsorbed for minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose. Based on ELISA, FLISA, and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody was tested by Dot Blot and/or solid phase adsorbed for minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgG1 covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG1. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with mouse IgM, IgG2a, IgG2b, IgG3, IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgG2b covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG2b. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with mouse IgM, IgG1, IgG2a, IgG3, IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using human IgG covalently linked to agarose beads. Based on ELISA, the antibody reacts with the heavy and light chains of human IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with bovine, horse, and mouse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose beads. Based on ELISA and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using human IgG covalently linked to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains of human IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with bovine, horse, and mouse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Genetex

Adipose tissue is an energy reserve in animals and is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice.

Supplier: BioVendor

Visceral adipose tissue – derived serpin A12 (vaspin), also named OL-64, an adipocytokine, is structurally a member of the serine protease family. Serpins are the most diverse family of protease inhibitors. Their typical structural feature is the core domain composed from 3 beta-sheets and 9 alpha-helixes. The inhibitory activity of vaspin has not been described up to now, but its reactive site loop is typical for this proteinase family. Human Vaspin protein is composed of 395 amino acids and has a molecular weight of approximately 45.2 kDa and predicted pI 9.26. The cDNA was first isolated from white adipose tissue of Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Vaspin mRNA expression is specific for visceral adipose tissues and it is also found circulating in the serum. The level of serum vaspin increased with age up to the peak of obesity, body weight and insulin resistance in OLETF rats and decreases with worsening of diabetes. Vaspin expression is missing in the diabetes-resistant lean rats, LETO, in comparison to OLEFT rats, animal model of metabolic syndrome. Expression was also absent in the subdermal, brown fatty tissue and other non-adipose tissues in OLEFT rats. These findings lead to the conclusion that the target tissue for insulin sensitising effect of vaspin is white adipose tissue. In humans, elevated serum concentration of vaspin is associated with obesity and impaired insulin sensitivity.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose beads. Based on ELISA and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using human IgG covalently linked to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains of human IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with bovine, horse, and mouse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose beads. Based on ELISA and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody was tested by Dot Blot and/or solid phase adsorbed for minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose beads. Based on ELISA and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody was tested by Dot Blot and/or solid phase adsorbed for minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using pooled IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgG2a covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG2a. This antibody was tested by Dot Blot and/or solid phase adsorbed for minimal cross-reactivity with mouse IgM, IgG1, IgG2b, IgG3, IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.