27727 Results for: "affinity chromatography"

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Nucleoprotein / NP ( Catalog#40444-V07E1; YP_138520.1; Gly630-Asp738). Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Nucleoprotein / NP specific IgG was purified by Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Nucleoprotein / NP affinity chromatography.

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Nucleoprotein / NP ( Catalog#40443-V07E1; AHX24646.1; His630-Gln739). Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Nucleoprotein / NP specific IgG was purified by Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Nucleoprotein / NP affinity chromatography.

Supplier: Biotium

CF™488A goat anti-human IgM (μ chain) is prepared from antibodies purified by human IgM-affinity chromatography. The antibody reacts specifically with IgM heavy chains (μ chains) and not with immunoglobulin light chains. To minimize crossreactivity, it has been adsorbed against human IgG prior to conjugation. Green fluorescent CF™488A (Ex/Em 490/515 nm) is a superior alternative to FITC (or fluorescein) owing to its exceptional brightness and photostability.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

Supplier: Cube Biotech

The SMALP products have been developed for biomedical purposes, in particular, for the solubilization of (biological) lipid membranes and isolation & purification of integral membrane proteins.
After solving the SMA + buffer powder in water, the product is ready-to-use. It can primarily used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: Biosensis

The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.

Supplier: Biosensis

The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.

Supplier: Cube Biotech

The SMALP products have been developed for biomedical purposes, in particular, for the solubilization of (biological) lipid membranes and isolation & purification of integral membrane proteins.
After solving the SMA + buffer powder in water, the product is ready-to-use. It can primarily used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: Cube Biotech

The SMALP products have been developed for biomedical purposes, in particular, for the solubilization of (biological) lipid membranes and isolation & purification of integral membrane proteins.
After solving the SMA + buffer powder in water, the product is ready-to-use. It can primarily used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: Cube Biotech

The SMALP products have been developed for biomedical purposes, in particular, for the solubilization of (biological) lipid membranes and isolation & purification of integral membrane proteins.
After solving the SMA + buffer powder in water, the product is ready-to-use. It can primarily used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: Anaspec

The renin–angiotensin system (RAS) plays a central role in the regulation of blood pressure and electrolyte homoeostasis. An overactive renin-angiotensin system leads to hypertension. As a result, renin is an attractive target for the treatment of this disease

Recombinant mouse prorenin is produced in HEK cells and purified by chelated metal affinity chromatography. It contains an 8x-Histidine tag at the C terminus. The apparent Mr of recombinant enzyme on SDS-PAGE is 41.7 kDa. Prorenin, the precursor of renin, is a glycosylated aspartic protease that consists of 2 homologous lobes. Prorenin can be activated with trypsin. Its activity can be measured in a FRET-based enzymatic assay

Supplier: Enzo Life Sciences

FLAG-tag stands for an octapeptide with a sequence of DYKDDDDK. It is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It is widely used for affinity chromatography to purify recombinant proteins from variety of systems. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence, such as in ELISA, Western Blot, etc.

Supplier: Cube Biotech

The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: Cube Biotech

The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: Cube Biotech

The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: Cube Biotech

The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: Anaspec

Myelin Oligodendrocyte Glycoprotein (MOG) is a member of the immunoglobulin superfamily and is expressed exclusively in the central nervous system (CNS). Although MOG protein constitutes only 0.01-0.05% of the CNS myelin proteins, it was demonstrated that MOG protein is a crucial autoantigen for multiple sclerosis in humans and experimental autoimmune encephalomyelitis (EAE) in rodents and monkeys

The sequence (Accession # NP_034944) corresponding to the extracellular domain of mouse MOG along with a 6x His tag was expressed in E. coli. The recombinant mouse MOG (M-rMOG) was purified from urea denatured bacterial lysate using immobilized metal affinity chromatography (IMAC). The molecular mass of the recombinant mouse MOG is 14.2 kDa

Supplier: Anaspec

Myelin Oligodendrocyte Glycoprotein (MOG) is a member of the immunoglobulin superfamily and is expressed exclusively in the central nervous system (CNS). Although MOG protein constitutes only 0.01-0.05% of the CNS myelin proteins, it was demonstrated that MOG protein is a crucial autoantigen for multiple sclerosis in humans and experimental autoimmune encephalomyelitis (EAE) in rodents and monkeys

The sequence (Accession #CAE84068) corresponding to the extracellular domain of rat MOG along with a 6x His tag was expressed in E. coli. The recombinant rat MOG (R-rMOG) was purified from urea denatured bacterial lysate using immobilized metal affinity chromatography (IMAC). The molecular mass of the recombinant rat MOG is 14.2 kDa.

Supplier: Anaspec

Myelin Oligodendrocyte Glycoprotein (MOG) is a member of the immunoglobulin superfamily and is expressed exclusively in the central nervous system (CNS). Although MOG protein constitutes only 0.01-0.05% of the CNS myelin proteins, it was demonstrated that MOG protein is a crucial autoantigen for multiple sclerosis in humans and experimental autoimmune encephalomyelitis (EAE) in rodents and monkeys

The sequence (Accession # CAQ10087) corresponding to the extracellular domain of human MOG along with a 6x His tag was expressed in E. coli. The recombinant human MOG (H-rMOG) was purified from urea denatured bacterial lysate using immobilized metal affinity chromatography (IMAC). The molecular mass of the recombinant human MOG is 14.2 kDa

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose. Based on ELISA, FLISA, and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody was tested by Dot Blot and/or solid phase adsorbed for minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgG1 covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG1. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with mouse IgM, IgG2a, IgG2b, IgG3, IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using human IgG covalently linked to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains of human IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with bovine, horse, and mouse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose. Based on ELISA, FLISA, and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody was tested by Dot Blot and/or solid phase adsorbed for minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgG2b covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG2b. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with mouse IgM, IgG1, IgG2a, IgG3, IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using human IgG covalently linked to agarose beads. Based on ELISA, the antibody reacts with the heavy and light chains of human IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with bovine, horse, and mouse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using human IgG covalently linked to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains of human IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with bovine, horse, and mouse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using human IgG covalently linked to agarose beads. Based on ELISA, the antibody reacts with the heavy and light chains of human IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with bovine, horse, and mouse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using human IgG covalently linked to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains of human IgG. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with bovine, horse, and mouse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.

Supplier: Bioss

The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The to Epac 1are affinity purified over immobilized antigen based chromatography.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose beads. Based on ELISA and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody was tested by Dot Blot and/or solid phase adsorbed for minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose beads. Based on ELISA and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.