27727 Results for: "affinity chromatography"

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant Human respiratory syncytial virus (RSV) (strain A2, strain Long) Nucleoprotein (Catalog#40821-V08E; P03418; Met1-Leu391). Human respiratory syncytial virus (RSV) (strain A2, strain Long) Nucleoprotein specific IgG was purified by Human respiratory syncytial virus (RSV) (strain A2, strain Long) Nucleoprotein affinity chromatography.

Supplier: Sino Biological

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL10/IL-10/Interleukin-10 (rh IL10/IL-10/Interleukin-10; Catalog#10947-HNAE; NP_000563.1; Ser 19-Asn 178). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Supplier: Genetex

Rabbit Polyclonal antibody to HSP90B

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) GP1 / Glycoprotein ( Catalog#40368-V08B; ACI28624.1; Met1-Arg501). Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) GP1 / Glycoprotein specific IgG was purified by Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) GP1 / Glycoprotein affinity chromatography.

Supplier: Sino Biological

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human GM-CSF / CSF2 (rh GM-CSF / CSF2; Catalog#10015-H07H; NP_000749.2; Ala 18-Glu 144). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and conjugated with horseradish-peroxidase (HRP).

Supplier: Sino Biological

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Nucleoprotein / NP ( Catalog#40443-V07E1; AHX24646.1; His630-Gln739). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Supplier: Sino Biological

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human KSP-Cadherin / Cadherin-16 / CDH16 (rh KSP-Cadherin / Cadherin-16 / CDH16; Catalog#10915-H08H; NP_004053.1; Met 1-Ala 786). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant H5N2 (A/American green-winge teal/California/HKWF609/07) HA / Hemagglutinin (Catalog#11699-V08H; ACF47563.1; Met 1-Gln 527). H5N2 (A/American green-winge teal/California/HKWF609/07) HA / Hemagglutinin specific IgG was purified by H5N2 (A/American green-winge teal/California/HKWF609/07) HA / Hemagglutinin affinity chromatography.

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant Influenza H18N11 (A/flat-faced bat/Peru/033/2010) Hemagglutinin/HA1 ( Catalog No: 40324-V08H1; AGX84934.1; Met1-Arg339). Influenza H18N11 (A/flat-faced bat/Peru/033/2010) Hemagglutinin/HA1 specific IgG was purified by Influenza H18N11 (A/flat-faced bat/Peru/033/2010) Hemagglutinin/HA1 affinity chromatography.

Supplier: Prosci

PAX-8 is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.

Supplier: Sino Biological

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat Coagulation Factor III / Tissue Factor / CD142 (rR Coagulation Factor III / Tissue Factor / CD142; Catalog#80407-R08H; Q66HI4; Met1-Glu252). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) VP40/Matrix ( Catalog No: 40448-V07E; YP_003815434.1; Ala31-Lys326). Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) VP40/Matrix specific IgG was purified by Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) VP40/Matrix affinity chromatography.

Supplier: Prosci

This antibody recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 antibody with a GCDFP-15 antibody is valuable in the diagnosis of apocrine carcinoma.

Supplier: Prosci

This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This mAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Supplier: Sino Biological

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TNF-beta / TNFSF1 / Lymphotoxin alpha (rh TNF-beta / TNFSF1 / Lymphotoxin alpha ; Catalog#10270-HNAE; P01374; Leu 35-Leu 205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Supplier: Prosci

Epidermal growth factor receptor (EGFR) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha. Upon activation by its growth factor ligands, EGFR undergoes a transition from an inactive monomeric form to an active homodimer. In addition to forming homodimers after ligand binding, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of several tyrosine (Y) residues in the C-terminal domain of EGFR occurs. This autophosphorylation elicits downstream activation and signaling by several other proteins that associate with the phosphorylated tyrosines through their own phosphotyrosine-binding SH2 domains. These downstream signaling proteins initiate several signal transduction cascades, principally the MAPK, Akt and JNK pathways, leading to DNA synthesis and cell proliferation. [Wiki]

Supplier: Sino Biological

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Endoglin / CD105 (rh CD105; Catalog#10149-H08H; Met 1-Gly 586; NP_001108225.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and conjugated with FITC under optimum conditions, the unreacted FITC was removed
.

Supplier: Sino Biological

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Matrix protein VP40 ( Catalog#40446-V07E; AHX24648.1; Asn31-Lys326). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Supplier: Prosci

Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1 (Catalog#40010-V07E; NP_040978.1; Ser2-Lys252). Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1 specific IgG was purified by Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1 affinity chromatography.

Supplier: MP Biomedicals

Pure Canavalia ensiformis lectin (Con A) from Jackbean. Isolated by affinity chromatography on cross-linked dextran. Con A exists as a dimer below pH 5.0 and and at pH >7 it exists as a tetramer. Con-A is not a glycoprotein. The monomeric molecular weight of Con-A is 25500. Con-A does not contain cysteine residues. Unlike most other lectins, Con-A is a metalloprotein and requires a transition metal ion, such as manganese, plus calcium ions for binding.

Supplier: Prosci

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known.

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant EBOV Ebola virus EBOV (subtype Sudan, strain Gulu) VP40 / Matrix protein VP40 (Catalog#40447-V07E; YP_138522.1; Val31-Lys326). Ebola virus EBOV (subtype Sudan, strain Gulu) VP40 / Matrix protein VP40 specific IgG was purified by EBOV Ebola virus EBOV (subtype Sudan, strain Gulu) VP40 / Matrix protein VP40 affinity chromatography.

Supplier: Prosci

This antibody is specific to a conserved determinant of the p53 protein. PAb 122 binds to the C-terminus (aa 370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb 122 blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). The protein in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Its the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.

Supplier: RPI

Protease inhibitor cocktail, EDTA-free tablets are designed to protect isolated proteins by providing an extraordinary inhibition of serine proteases, cysteine proteases, and aminopeptidases during extractions from animal and plant tissues or cells, yeast, and bacteria. The provided tablets do not contain EDTA, leaving the stability and the function of metal-depended proteins unaffected. Contains both irreversible and reversible protease inhibitors. Aspartic and metallo proteases are not inhibited with the exception of aminopeptidases. The affinity purification of Poly-His tagged fusion proteins via IMAC (immobilized metal ion affinity chromatography) is also facilitated (no dialysis necessary). Tablets contain AEBSF, Aprotinin, E-64, and Leupeptin hemisulfate.

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant Influenza H3N2 (A/reassortant/IVR-155(Victoria/210/2009×Puerto Rico/8/1934)) Hemagglutinin/HA1 ( Catalog No: 40058-V08H1; ADI52838.1; Met1-Arg345). Influenza H3N2 (A/reassortant/IVR-155(Victoria/210/2009×Puerto Rico/8/1934)) Hemagglutinin/HA1 specific IgG was purified by Influenza H3N2 (A/reassortant/IVR-155(Victoria/210/2009 x Puerto Rico/8/1934)) Hemagglutinin/HA1 affinity chromatography.

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) VP24 ( Catalog#40454-V07E; AHX24653.1; Met1-Gly233). Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) VP24 specific IgG was purified by Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) VP24 affinity chromatography.

Supplier: Li-Cor

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgG2a covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG2a. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with mouse IgM, IgG1, IgG2b, IgG3, IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications.

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) VP24 ( Catalog#40454-V07E; AHX24653.1; Met1-Gly233). Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) VP24 specific IgG was purified by Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) VP24 affinity chromatography.

Supplier: Sino Biological

Produced in rabbits immunized with purified, recombinant Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) VP24 Protein ( Catalog#40454-V07E; AHX24653.1; Met1-Gly233). Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) VP24 specific IgG was purified by Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) VP24 affinity chromatography.