2265 Results for: "activating reagent"

Supplier: Genetex

This highly active polyclonal antibody is directed against purified recombinant Glutathione-S-Transferase (GST) cloned from the organism Schistosoma japonicum. This reagent is specifically designed for screening and recovery of fusion proteins from expression systems using a GST gene cloning site.

Supplier: EMPIRICAL BIOSCIENCE

RTScript™ cDNA Transcriptase is a Modified version of M-MLV Reverse Transcriptase with RNase H activity deactivated and increased thermal stability. In combination with optimized buffers and reagents, Empirical's RTScript™ enzyme synthesizes complementary DNA from RNA initiated from RNA or DNA Primers.

Supplier: Spectrum Chemical Mfg. Corp.

Lanthanum Chloride, Hydrated, Reagent, ACS, is used in biochemical research to block the activity of divalent cation channels, mainly calcium channels. Doped with cerium, it is used as a scintillator material and in organic synthesis, lanthanum trichloride functions as a mild Lewis acid for converting aldehydes to acetals. As an ACS grade quality reagent, its chemical specifications are the de facto standards for chemicals used in many high-purity applications and typically designate the highest quality chemical available for laboratory use. Spectrum Chemical manufactured Reagent ACS grade products meet the toughest regulatory standards for quality and purity.

Supplier: Genetex

This highly active polyclonal antibody is directed against purified recombinant Glutathione-S-Transferase (GST) cloned from the organism Schistosoma japonicum. This reagent is specifically designed for screening and recovery of fusion proteins from expression systems using a GST gene cloning site

Supplier: Genetex

This highly active polyclonal antibody is directed against the bacterial form of the enzyme and may be used to detect any beta-galactosidase expression by the lacZ gene. This reagent is specifically designed for screening and recovery of fusion proteins from expression systems with lacZ gene cloning sites.

Supplier: Genetex

This highly active polyclonal antibody is directed against purified recombinant Glutathione-S-Transferase (GST) cloned from the organism Schistosoma japonicum. This reagent is specifically designed for screening and recovery of fusion proteins from expression systems using a GST gene cloning site.

Supplier: Enzo Life Sciences

Protoporphyrin IX free acid, as distinct from its zinc salt (which inhibits heme oxygenase), activates soluble guanylyl cyclase (sGC) by binding directly to the enzyme. A useful reagent in cases where the use of nitric oxide (NO) or nitric oxide donors is undesirable.

Supplier: Genetex

This highly active polyclonal antibody is directed against the bacterial form of the enzyme and may be used to detect any beta-galactosidase expression by the lacZ gene. This reagent is specifically designed for screening and recovery of fusion proteins from expression systems with lacZ gene cloning sites.

Supplier: Spectrum Chemical Mfg. Corp.

Potassium Chlorate, Crystal, Reagent, ACS has been used as an oxidizing agent, a disinfectant, in oxygen preparation, and in the detection of aluminum. This simple ionic salt can yield free chlorate ions in solution which is thought to be the species responsible for this versatile activity. As an ACS grade Reagent, Spectrum Chemical manufactured compounds are used as the quality standard against which other substances are graded and has met the toughest regulatory standards for quality and pureness

Supplier: Enzo Life Sciences

The IHC tissue primer is a universal enzyme blocking reagent to use in horse radish peroxidase (HRP) and alkaline phosphatase (AP) based immunohistochemical staining procedures done on paraffin tissue sections, frozen tissue sections and cell smears to suppress nonspecific staining due to endogenous enzyme activity.

Supplier: Diagnostic Biosystems

Tissue Primer is a universal enzyme blocking reagent to use in horse radish peroxidase (HRP) and alkaline phosphatase (AP) based immunohistochemical staining procedures done on paraffin tissue sections, frozen tissue sections and cell smears to suppress nonspecific staining due to endogenous enzyme activity.

Supplier: Bioss

Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells.HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.

Supplier: Bioss

Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells.HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.

Supplier: ARBOR ASSAYS MS

The P450 Demethylating Fluorescent Activity Kit quantifies formaldehyde released during enzymatic demethylation by cytochrome P450s such as CYP3A4, 2D6, and 2B4. Using a fluorescence-based detection reagent, the assay measures formaldehyde production, a direct readout of P450 activity. This kit is ideal for in vitro reconstituted P450 systems, including microsomes, Cerosomes™, or recombinant enzymes. With a detection range of 12.5 to 400 µM, the assay supports screening of P450 activity, enzyme kinetics, and inhibitor potency. The 45-minute mix-and-read protocol simplifies workflow and enables high-throughput testing of drug metabolism and xenobiotic interactions.

Supplier: Abcam

MTS reagent for cell proliferation assay. Tetrazolium inner salt is used for determining cell viability in proliferation assays. Reduced in metabolically active cells to a colored formazan product that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes. - Purity >98% - Soluble in DPBS to 2 mg/ml

Supplier: Promega Corporation

Homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome in a purified enzyme-based format. Adding the Proteasome-Glo™ Reagent in an "add-mix-measure" format results in proteasome cleavage of the substrate and rapid generation of a luminescent signal produced by the luciferase reaction.

Supplier: ARBOR ASSAYS MS

The Histone Demethylase Fluorescent Activity Kit from Arbor Assays enables rapid detection of formaldehyde, a byproduct of histone demethylation by LSD1- and Jumonji-type HDMs. The assay measures fluorescence generated upon formaldehyde reaction with a specialized detection reagent, allowing real-time quantification of HDM activity. With a detection range of 3.125 to 200 µM, this versatile kit accommodates in vitro assays and cell lysates and is adaptable for studying HDM inhibitors, cofactor dependencies, and epigenetic modulation. Its simple 30-minute workflow after demethylation makes it ideal for high-throughput screening or detailed kinetic characterization of histone demethylase activity.

Supplier: Peprotech

The MCP proteins belong to the CC chemokine family, and signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated T cells, basophils, NK cells, and immature dendritic cells. The MCP family cross reacts across species. Recombinant human MCP-1 is an 8.6 kDa protein containing 76 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines. Manufactured using all Animal-Free reagents.

Supplier: Invitrogen

Thermo Scientific Pierce MS(PEG)_n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)₁₂ is a three-branched, polyethylene glycol compound (3 x 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).

Supplier: Rockland Immunochemical

ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta). ERK1 Protein is ideal for investigators involved in Signaling Reagents, Protein Substrates, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, and Ser/Thr Kinases research.

Supplier: Adipogen

Fluorescent probe and pH indicator. It fluoresces blue in acidic solutions and in acidic organelles but fluoresces green in more basic organelles. This compound is potentially useful as a coloring agent, biological stain or optical detecting reagent. It is used in water-in-oil-in-water emulsions and as a substrate for lipase activity. Spectral data: lambdaex 375nm, lambdaem 404nm in water.

Supplier: Abcam

Hydrogen peroxide blocking reagent is used to block endogenous peroxidase activity in IHC applications, enhancing signal detection in HRP / peroxidase-based systems. - Cited in over 30 publications - Endogenous peroxidase block – prevents non-specific background staining in IHC - HRP-based detection – ideal for use with HRP/peroxidase detection systems - Concentration – contains 0.3% hydrogen peroxide

Supplier: ARBOR ASSAYS MS

The Butyrylcholinesterase Fluorescent Activity Kit from Arbor Assays provides a rapid, sensitive method for detecting BChE activity in serum and plasma. Using the proprietary ThioStar® detection reagent, the kit quantifies reduced thiol products generated during butyrylcholine hydrolysis. With a detection range of 0.313 to 20 mU/ml and sensitivity down to 0.018 mU/ml, the assay delivers results in just 20 minutes. The species-independent format is ideal for studying drug metabolism, neurodegenerative disease, and cholinesterase inhibitor screening, with minimal cross-reactivity to acetylcholinesterase.

Supplier: Aladdin Scientific

It is used as a skin moisturizer lotion to treat dry, scaly, itchy skin, reagent in biological activity studies of aluminum and in sol-gel processing. Also used in fire foam. Aluminum L-Lactate has been used to inject aluminum into rabbits. Aluminum L-Lactate has been shown to decrease human enamel dissolution in a pH 4 acetate buffer.

Supplier: Invitrogen

Photoactivatable Biotin, Biotin-LC-ASA, Psoralen-PEG3-Biotin and TFPA-PEG3-Biotin each contain a photoactivatable group. When exposed to UV light, they become activated and insert non specifically into nearby molecules. These reagents may be used to label proteins and peptides, but they are also useful in labeling DNA, RNA and other molecules that do not contain any readily reactive functional groups.

Supplier: Spectrum Chemical Mfg. Corp.

Polyethylene Glycol 40 Castor Oil, Hydrogenated is a polyether compound that is used in a wide variety of fields including pharmaceutical manufacturing as an excipient and active ingredient. Due to its low toxicity it can be used as a lubricating coating for various surfaces in aqueous and non-aqueous environments, a reagent in biochemistry to create very high osmotic pressures, a polar stationary phase for gas chromatography and as a binder.

Supplier: Spectrum Chemical Mfg. Corp.

Polyethyleneglycol 60 Castor Oil, Hydrogenated is a polyether compound that is used in a wide variety of fields including pharmaceutical manufacturing as an excipient and active ingredient. Due to its low toxicity it can be used as a lubricating coating for various surfaces in aqueous and non-aqueous environments, a reagent in biochemistry to create very high osmotic pressures, a polar stationary phase for gas chromatography and as a binder.

Supplier: Bioss

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Supplier: Bioss

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.