Supplier: Promega Corporation

The Wizard SV 96 Genomic DNA Purification System provides a high-throughput, membrane-based technique for preparation of genomic DNA from cultured cells and tissue, including mouse tails.

Supplier: Promega Corporation

The Wizard SV 96 Genomic DNA Purification System provides a high-throughput, membrane-based technique for preparation of genomic DNA from cultured cells and tissue, including mouse tails.

Supplier: Spectrum Chemical Mfg. Corp.

Sodium Tartrate, Dihydrate, Crystal, Reagent, ACS has a crystal structure that is able to capture a very precise amount of water and is used in the Karl Fischer moisture determination titration technique. As an ACS grade reagent, Spectrum Chemical manufactured compound is used as the quality standard against which other substances are grade and has met the toughest regulatory standards for quality and pureness

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

L(+)-Histidine monohydrochloride monohydrate 98.5-101.0% (dried basis), Multi-Compendial, Macron Fine Chemicals™

Supplier: Molecular Devices

Transform your whole colony picking process by eliminating the risk of double picks or blank wells, reducing manual picking errors and avoiding cross-contamination. With a QPix® system, you can rely on advanced imaging and analysis combined with high precision robotics to pick the right colony every time. Highly robust performance ensures data quality and high viability of picked colonies.

Supplier: Spectrum Chemical Mfg. Corp.

Cysteine Hydrochloride, Monohydrate, USP is a semi-essential amino acid. 

Supplier: Spectrum Chemical Mfg. Corp.

Sodium Potassium Tartrate, Tetrahydrate, Granular, FCC is used as a sequestrant and stabilizer. The FCC grade meets the requirements of the Food Chemical Codex indicates and is suitable for all food, beverage and nutritional supplement applications. Spectrum Chemical offers over 300 Food grade chemical ingredients packaged in laboratory size bottles to production drum quantities and are manufactured, packaged and stored under current Good Manufacturing Practices (cGMP) per 21CFR part 211 in FDA registered and inspected facilities.

Supplier: Spectrum Chemical Mfg. Corp.

L-Cysteine Monohydrochloride, Monohydrate, FCC - The FCC grade meets the requirements of the Food Chemical Codex indicates and is suitable for all food, beverage and nutritional supplement applications. Spectrum Chemical offers over 300 Food grade chemical ingredients packaged in laboratory size bottles to production drum quantities and are manufactured, packaged and stored under current Good Manufacturing Practices (cGMP) per 21CFR part 211 in FDA registered and inspected facilities.

Supplier: Spectrum Chemical Mfg. Corp.

Sodium Potassium Tartrate, Tetrahydrate, Powder, FCC is used as a sequestrant and stabilizer. The FCC grade meets the requirements of the Food Chemical Codex indicates and is suitable for all food, beverage and nutritional supplement applications. Spectrum Chemical offers over 300 Food grade chemical ingredients packaged in laboratory size bottles to production drum quantities and are manufactured, packaged and stored under current Good Manufacturing Practices (cGMP) per 21CFR part 211 in FDA registered and inspected facilities.

Supplier: MP Biomedicals

Potassium sodium tartrate tetrahydrate has been used in organic synthesis to break up emulsions in aqueous workups.

Supplier: Spectrum Chemical Mfg. Corp.

Antimony Potassium Tartrate, Powder, USP is also referred to as emetic tartar. It has long been known as a powerful emetic. All Spectrum Chemical USP products are manufactured, packaged and stored under current Good Manufacturing Practices (cGMP) per 21CFR part 211 in FDA registered and inspected facilities

Supplier: Spectrum Chemical Mfg. Corp.

L-Histidine Monohydrochloride, Monohydrate, also known as 2-Amino-3-(1H-imidazol-4-yl)propanoic acid, is an alpha-amino acid with an imidazole functional group.

Supplier: MP Biomedicals

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature