46850 Results for: "Stainers and Equipment, Electron Microscopy Sciences"

Supplier: Rockland Immunochemical

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

Supplier: Rockland Immunochemical

Fibrillarin Antibody detects Nop1p which was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. Anti-Fibrillarin Antibody is ideal for investigators involved in Cell Proliferation and Cancer Research.

Supplier: 3M Healthcare

CUNO™ CTG-Klean System Filter Pack with Betapure™ NT-T Series Cartridge is an enclosed system that uses a separate pressure vessel and disposable filter pack to isolate the product to be filtered from the housing. It reduces the labor and operator exposure to impurities and cleaning solvents involved with filter change-outs and cleaning of conventional housings.

Supplier: ELPRO SERVICES, INC.

ELPRO's next generation of INDEPENDENT monitoring solutions for temperature measurement with external sensors. The ideal −80 °C ULT Freezer data logger kit to simplify the storage of your valuable products. A convenient 'pause alarm' feature allows users to temporarily turn off the alarming e.g. during cryogenic LN₂ tank refills, but still maintain traceability. The data logger kit is reconfigurable and reusable for up to three years and are 100% GxP compliant.

Supplier: Adipogen

IL-36Ra/IL-1F5 is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to IL-36alpha (IL-1F6), IL-36beta (IL-1F8) and IL-36gamma (IL-1F9). These cytokines bind to IL-36R (IL-1Rrp2) and IL-1RAcP, activating similar intracellular signals as IL-1. IL-36Ra inhibits the production of proinflammatory cytokines, including IL-12, IL-1beta, IL-6, TNF-alpha and IL-23 induced by IL-36 in BMDC and CD4 T cells. Skin and dendritic cells are target of the IL-36 interleukins leading to a Th1 response. Recently mutations that affect the levels and the activity of IL-36Ra have been found in patients with pustular psoriasis, leading to enhanced production of inflammatory cytokines (IL-8 in particular) by keratinocytes.

Supplier: Adipogen

Cochlin is an extracellular matrix protein expressed in the cochlea and vestibule of the inner ear. Mutations in the cochlin gene are linked to the autosomal-dominant DFNA9 syndrome, a disorder characterized by hearing loss. Cochlin is also expressed in follicular dendritic cells of the spleen and the lymph nodes. The N-terminus of cochlin contains a LCCL domain also present in Factor C, a serine protease of the horseshoe crab Limulus, involved in activating the coagulation cascade in response to LPS. A recent study showed that in response to inflammation, LPS or bacterial infection, cochlin is cleaved by the proteases aggrecanases and secreted into blood circulation. The secreted cochlin (LCCL domain) seems to have a role in local cytokine production, recruitment of immune effector cells and bacterial clearance. Cochlin is secreted as two forms p8 and p18, whereas p18 being the glycosylated form of p8.

Supplier: MP Biomedicals

IPTG (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli.
IPTG is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal in blue-white color selection of recombinant bacterial colonies.
The final concentration typically used in indicator plates is 0.2 mM.

Supplier: Adipogen

Betatrophin (RIFL; Lipasin; Angiopoietin-like protein 8 (ANGPTL8)) is a newly discovered secreted protein of 198 aa that was proposed to promote beta cell proliferation and improve glucose tolerance in mice. Betatrophin may also function in inhibition of lipase activity and on serum triglyceride regulation. Betatrophin is expressed in the liver and in white and brown adipose tissue of mice. In humans, betatrophin is found to be predominantly expressed in the liver. Betatrophin levels are reduced by fasting and are elevated upon insulin resistance and during pregnancy. Betatrophin, according to preliminary data could bind to the macrophage receptor Marco and also to RTN4R, a neuronal receptor. Recently, a study using ANGPTL8 KO mice showed that ANGPTL8/Betatrophin does not play a role in beta cell proliferation nor in glycemic control as previously thought, but regulates plasma triglyceride levels.

Supplier: Adipogen

Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Supplier: Adipogen

Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localized to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues and is expressed at high levels in skeletal muscle, heart, lung and kidney and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed.

Supplier: ELPRO SERVICES, INC.

ELPRO's next generation of INDEPENDENT monitoring solutions for temperature measurement with external sensors. The ideal LN2 Freezer data logger kit to simplify the storage of your valuable products. A convenient 'pause alarm' feature allows users to temporarily turn off the alarming e.g. during cryogenic LN2 tank refills, but still maintain traceability. The data logger kit is reconfigurable and reusable for up to three years and are 100% GxP compliant.

Supplier: Eppendorf

The Innova 44 and 44R (refrigerated) are Eppendorf’s most popular shakers - they accommodate flasks up to 5 L, have the space-saving glide-up door, and the reliable Innova triple-eccentric drive. They just run forever to give you peace of mind.

Supplier: PHC Corporation

PHCbi Biomedical ECO Freezers provide a stable and reliable environment for storage of enzymes, vaccines, pharmaceutical products and other biomedical materials with uniform temperatures.

Supplier: MP Biomedicals

L-Glutamine, the uncharged and amidated analog of L-glutamic acid, is an important amino acid for the incorporation of NH4+ into biomolecules. It is biosynthesized from NH4+ and glutamate via the enzyme glutamate synthetase. In turn, degradation of glutamine to free the ammonia moiety is mediated by glutaminase. Glutamine also participates in acid-base regulation in vivo.

Supplier: MP Biomedicals

SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions.

Supplier: Adipogen

Human hepatocyte growth factor (HGF) is synthesized as a biologically inactive single chain precursor, which is cleaved by a specific, extracellular serum serine protease to a fully active heterodimer. It stimulates the motility and invasion of several cancer cell types and can induce angiogenesis. All biological responses induced by HGF are elicited by binding to its transmembrane tyrosine kinase receptor. HGF function is essential for normal development. Knockout studies have demonstrated that both ligand and receptor deficient mice display an embryonic lethal phenotype. HGF synergizes with basic FGF in the induction of angiogenesis. HGF is a potent mitogen for mature parenchymal hepatocyte cells and is a pleiotrophic cytokine. It acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity. It is an activating ligand for the receptor tyrosine kinase MET by binding and promoting its dimerization.

Supplier: Adipogen

BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation . Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases, such as Sjögren’s syndrome, Rheumatoid Arthritis (RA), Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE) . BAFF is also increased levels in some lymphoid cancers.

Supplier: Adipogen

Interleukin-22 (IL-22), also known as IL-10 related T cell-derived-inducible factor (ILTIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R(CRF29) and IL-10Rbeta(CRF24), belonging to the class II cytokine receptor family.

Supplier: Adipogen

CD134 (OX40) is a T cell activation antigen structurally belonging to a lymphocyte-specific subgroup of the nerve growth factor and tumor necrosis factor receptor superfamily, which also includes the T cell antigen CD27, B cell antigen CD40, FAS antigen and the T cell activation antigen 4-1BB. The human CD134 protein is expressed only on activated CD4+ T blasts, and its ligand has been identified as gp34. Interactions between CD134 and its ligand in vivo are necessary for the differentiation of activated B cells into highly immunoglobulin-producing cells, however not involved in other pathways of antigen-driven differentiation of B cells such as development of memory cells in the germinal centers. In addition, the CD134 and gp34 system directly mediate adhesion of activated T cells to vascular endothelial cells, and contribute to growth stimulation of the virus-infected T cells.

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry spike protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations identified in Beta variant: L18F, D80A, D215G, L242-244del, R246I, K417N, E484K, N501Y, D614G, and A701V. The spike protein has a 18-aa cytoplasmic tail truncation for optimal infection.

Supplier: Adipogen

anti-EGFP, monoclonal antibody (recombinant) (G3) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Supplier: BONE CLONES HOLDINGS

Examine anatomical differences based on diet.

Supplier: Adipogen

CCL2, also called monocyte chemotactic protein-1 (MCP-1), is a member of the C-C or beta chemokine family that is best known as a chemotactic agent for mononuclear cells. Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source. In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils that express its receptor CCR2. Additionally, it promotes Th2 polarization in CD4+ T cells. CCL2-mediated recruitment of monocytes to sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma.

Supplier: Thermo Scientific

The Thermo Scientific Megafuge™ 8 centrifuge provides exceptional capacity in a compact design with a smart, simple interface and the flexibility to support both clinical and research applications. The centrifuge fits in to space, with its compact footprint, and to applications, accomodating multi-laboratory settings.

Supplier: Elma

The dependable, state-of-the-art 37 kHz Elmasonic E Plus series includes 9 tank sizes with simple, user-friendly operation. Samples are rapidly mixed, dispersed, dissolved, or homogenized in Dynamic mode, while Eco mode offers quiet gentle cleaning at reduced power.

Supplier: NABERTHERM INC.

These weighing furnaces with integrated precision scale and software, were designed especially for determining combustion loss in the laboratory. The determination of combustion loss is necessary, for instance, when analyzing sludges. Combustion loss is defined as the difference between the initial total mass and the combustion residue. During the process the software supplied records both temperature and weight loss.

Supplier: VWR

The VWR® PCR thermal cycler XT⁹⁶ combines industry-inspired innovation with the reliable quality of German manufacturing – housing both in a compact, low noise ventilation design that fits on virtually any bench top. For optimising PCR's, the XT⁹⁶ is also available with a gradient function.
The VWR® PCR thermal cycler XT⁹⁶ offers a powerful, yet easy to use software interface, as well as a host of other innovative features.

Supplier: ELPRO SERVICES, INC.

ELPRO's next generation of INDEPENDENT monitoring solutions for temperature measurement with external sensors. The ideal −20 °C Freezer data logger kit to simplify the storage of your valuable products. A convenient 'pause alarm' feature allows users to temporarily turn off the alarming e.g. during cryogenic LN2 tank refills, but still maintain traceability. The data logger kit is reconfigurable and reusable for up to three years and are 100% GxP compliant.

Supplier: MilliporeSigma

The Aquastar® range is designed to provide reagents for the determination of water by Karl Fischer titration of a wide range of substrates and by a variety of methodologies. These include reagents for one- or two-component systems; combined coulometric reagents; solvents for oils and fats; reagents and solvents for determination of water in ketones and aldehydes. Calibration standards are also available.

Supplier: Koehler

Constant temperature water baths designed for Reid Vapor Pressure determinations of liquid petroleum products and liquefied petroleum gases (LPG).