46850 Results for: "Stainers and Equipment, Electron Microscopy Sciences"

Supplier: Adipogen

8-hydroxy-2'-deoxyguanosine (8-OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals (incl. singlet oxygen and direct photodynamic action) that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8-OHdG is popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA and can be detected in tissue, serum, urine and other biomaterials.

Supplier: Adipogen

Interleukin-35 (IL-35) is a novel IL-12 family cytokine produced by regulatory T cells (Treg) but not by resting or activated effector T cells (Teff). IL-35 is a heterodimeric protein composed of EBI3 (Epstein-Barr-Virus-induced gene 3) and IL-12a (p35). EBI3 is a downstream target of Foxp3, a transcription factor required for Treg-cell development and function, and thus Treg-cell restriction of IL35 occurs. Regulatory T cells are essential for maintaining self tolerance and preventing autoimmunity, and IL-35 is identified as a molecule that mediates the immune suppression function of Treg-cell. As an inhibitory cytokine, IL-35 induces proliferation of Treg-cell populations but suppresses Th17 cell development. Studies in mice show the absence of either IL-35 chain from Treg-cell reduces the cells' ability to suppress inflammation using an experimental model for inflammatory bowel disease. IL-35 is suggested as a potential target of immunotherapy.

Supplier: Adipogen

B7-H4 is a B7 family member that negatively regulates T cell immunity by inhibiting of T cell proliferation, cytokine production and cell cycle progression. In vitro, B7-H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production and generation of alloreactive cytotoxic T cells (CTLs). In vivo, blockade of endogenous B7-H4 by specific monoclonal antibody promotes T cell responses. B7-H4 ia an important negative regulator of innate immunity through growth inhibition of neutrophils. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance.

Supplier: Adipogen

bHLH proteins are essential for neurogenesis, myogenesis, hematopoiesis and sex determination. HES1 (Hairy and Enhancer of Split 1) is a basic Helix-Loop-Helix (bHLH) transcriptional repressor regulated by the Notch pathway and expressed in various tissues. HES1 is well known in development of different tissues, particularly endoderm derived endocrine organs like pancreas and neuroendocrine cells of lung. HES1 controls normal development by regulating proliferation and differentiation and so cell fate. Notch signaling plays both oncogenic and tumor suppressor roles depending on cell type. In contrast to T cell acute lymphoblastic leukemia (ALL), where Notch activation promotes leukemogenesis, induction of Notch signaling in B cell ALL (B-ALL) leads to growth arrest and apoptosis. The Notch target HES1 is sufficient to reproduce this tumor suppressor phenotype in B-ALL by interacting and activating PARP-1 and regulating proapoptotic signals.

Supplier: Adipogen

Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p18, respectively, which leads to the generation of the active caspase-8 heterotetramer (p18/p10)2.

Supplier: Adipogen

Notch signaling pathway regulates many different cell fate decisions in both vertebrate and invertebrate species. There are 5 canonical Notch ligands in mammals: Jagged-1, Jagged-2, DLL1, DLL3 and DLL4. These can bind to the four Notch receptors Notch 1-4. It is important for pattern formation during development such as neurogenesis, angiogenesis or myogenesis and regulates T cell development and stem cell maintenance. Notch signaling is also involved in cellular processes through-out adulthood. Signaling via Notch occurs between neighbouring cells and both the receptor and its ligands are transmembrane proteins.

Supplier: Charles River Laboratories Cell Solutions

Peripheral blood cells are collected from healthy donors by direct collection or by using the Spectra Optia® Apheresis system. Cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: Adipogen

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Supplier: Adipogen

CD279 (Programmed Cell Death Protein 1; PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses. Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA-4 and PD-1). CD279 is expressed on activated T cells, B cells, myeloid cells and on a subset of thymocytes. In vitro, ligation of CD279 inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10 and IFN-gamma. In addition, CD279 ligation also inhibits BCR mediated signaling. CD279 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases.

Supplier: Adipogen

Caspase-11 not only activates caspase-1, that is required for the maturation of proinflammatory cytokines such as interleukin-1 (IL-1) and interleukin-18 (IL-18), but also activates caspase-3, leading to cellular apoptosis under pathological conditions. In most cells, caspase-11 is only expressed upon induction with pro-inflammatory stimuli. Caspase-11 is an essential mediator of endotoxic shock and caspase-11-deficient mice are resistant to endotoxic shock. LPS-induced caspase-11 regulates lymphocyte apoptosis by activating both caspase-3 and caspase-7.

Supplier: Adipogen

Stem cell factor (SCF), also known as cKit ligand (KL), mast cell growth factor (MGF) and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/cKit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.

Supplier: ARBOR ASSAYS MS

The Galactose Colorimetric Detection Kit from Arbor Assays provides sensitive and specific quantification of galactose in serum, plasma, tissue culture media, and buffers. Utilizing a galactose oxidase reaction coupled to HRP-mediated color development, the assay detects galactose concentrations down to 0.493 mg/dL over a 0.781 to 25 mg/dL range. With a fast, 30-minute protocol and low sample volume requirement, the kit is ideal for studies of metabolic regulation, galactosemia, glycolipid turnover, and nutritional biochemistry across multiple research models.

Supplier: SW Safety Solutions

These heavy-duty, latex-free, neoprene gloves provides superior resistance to chemicals, punctures, and abrasions with excellent grip, optimum tactility, and an extended cuff for splash protection.

Supplier: INKSMITH LIMITED SE

STEAM education for a brighter future.

Supplier: Adipogen

Interleukin-21 (IL-21) is a key factor in the transition between innate and adaptive immune responses secreted by activated T cells. The IL-21 receptor (IL-21R) is expressed in lymphoid tissue, in particular by NK, B, T and dendritic cells, macrophages and endothelial cells. Recent evidence suggests that IL-21 plays a supportive role in the proliferation of T and B cells and influences the cytolytic activity of natural killer cells. IL-21 has been shown to up-regulate genes associated with innate immunity and to inhibit the differentiation of naïve T helper cells. IL-21 specifically inhibits IFN-gamma production from developing TH1 cells and is preferentially expressed by TH2 cells. Furthermore IL-21 has been identified as a growth and survival factor for human myeloma cells. IL-21/IL-21R interactions have a unique role in sequentially activating both innate and adaptive immune responses against poorly immunogenic tumors, leading to tumor rejection that is perforin dependent but IFN-gamma independent.

Supplier: MP Biomedicals

CellMaxx™ Bovine Albumin is chromatographically purified from New Zealand-sourced bovine plasma in an ISO quality system assuring complete traceability and consistent high quality. This albumin has very low endotoxin and low IgG to simplify downstream purification, is tested for the absence of viruses according to 9CFR and has the highest possible biosafety level. The albumin supports growth of stem cells and increases protein production from hybridoma cells.

Supplier: Adipogen

Interleukin-15 (IL-15) has a broad spectrum of biological activities. It is crucial for the development, proliferation, survival and differentiation of multiple cells from both innate and adaptive immune systems. IL-15 up-regulation has a central role in the development of several autoimmune or chronic inflammatory disorders. Targeting IL-15 or its receptor may have a valuable impact on the treatment of immune-mediated diseases. IL-15 participates in the development of important immune antitumor mechanisms. It activates CD8(+) T cells, natural killer (NK) cells, NK T cells, and can promote the formation of antitumor antibodies. IL-15 can also protect T effector cells from the action of T regulatory cells and reverse tolerance to tumor-associated antigens. In pre-clinical studies IL-15 has been found to demonstrate potentiated antitumor effects following pre-association with IL-15Ralpha, or when used in combination with chemotherapy, adoptive therapy, monoclonal antibodies, and tumor vaccines.

Supplier: ARBOR ASSAYS MS

Arbor Assays' Insulin ELISA Kit enables rapid and reliable quantification of insulin in serum, plasma, and tissue culture media using a sensitive sandwich immunoassay format. This assay detects as low as 48.8 pg/ml and covers a linear detection range from 100 to 1600 pg/ml, offering high accuracy for clinical and research applications. Validated for human, bovine, and porcine insulin, the kit shows minimal cross-reactivity with rodent insulin or related peptides. With a 2 hours workflow, this kit is ideal for studies on glucose metabolism, diabetes, endocrine function, and insulin signaling.

Supplier: Adipogen

Fibroblast growth factors (FGFs) constitute a family of heparin-binding polypeptides involved in the regulation of biological responses such as growth, differentiation and angiogenesis. The biological effects of FGFs are mediated by four structurally related receptor tyrosine kinases denoted FGFR1, FGFR2, FGFR3 and FGFR4. FGF-1 is a powerful mitogen of cells of mesodermal, ectodermal and endodermal origin. FGF-1 association with heparan sulfate is a prerequisite for activation of FGF receptors. FGF-1 plays a role in various stages of development and morphogenesis as well as in angiogenesis and wound healing processes. Recent data indicate a role of FGF-1 in inflammation and obesity. FGF-1 is selectively induced in fat cells by high-fat diet feeding and established the PPARgamma-FGF-1 axis as a critical pathway that regulates adipose tissue remodeling.

Supplier: Adipogen

Reactive nitrogen species (RNS) are formed in a variety of diseases such as atherosclerosis, inflammation, cancer and neurodegenerative diseases. Peroxinitrite (ONOO-) is known as a potent biological oxidant, which is formed via very fast reaction between nitric oxide (NO) and superoxide (O2-). Peroxinitrite can induce nitration of aromatic amino acids such as Tyr, Trp, Phe and His, which results in modulation of protein functions. To date, 6-nitrotryptophan (6-NO2-Trp) is shown to be one of the most stable and major peroxynitrite-modified amino acids, and growing evidences suggest that nitrotryptophan may play a significant role in the cellular regulation under nitrosative stress. This antibody is suitable for immunohistochemistry, western blotting and ELISA and may provide a powerful tool for the investigation of nitrosative stress inside living bodies.

Supplier: Rockland Immunochemical

GbetaL (G protein beta protein subunit-like) is a member of a signaling pathway that regulates mammalian cell growth in response to the presence of nutrients and growth factors. It binds to the kinase domain of TOR (Target of rapamycin, also known as mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. TOR is normally associated with GbetaL and an additional regulatory protein RAPTOR, allowing TOR to control protein biosynthesis. The binding of GbetaL to TOR stimulates TOR's kinase activity towards downstream proteins such as RPS6K (ribosomal protein S6 kinase) and the translation factor 4E-BP1 which leads to increased protein translation and cell growth.

Supplier: Rockland Immunochemical

Anti-Golden Syrian Hamster IgG Cy3.5 Antibody generated in goat detects Golden Syrian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Supplier: Greiner Bio-One

Designed for use in the examination of whole blood in hematology applications, EDTA tubes feature interior walls that are coated with either K2EDTA or K3EDTA.

Supplier: Hach

In 24 hours, Hach's m-ColiBlue24® broth give the simultaneous results for total coliforms and E. coli. Hach's ready-to-use media eliminates measuring, mixing, and autoclaving steps necessary to prepare dehydrated media. Glass ampules, plastic ampules, 100 ml bottles of m-ColiBlue24® and agar plates, afford maximum shelf life and ease of use for pre-measured and prepared media. Enumerate total coliforms and E. coli on one petri dish. Differentiate colonies easily - red and blue indicate total coliforms and blue specifies E. coli. Get optimal recovery of stressed and injured organisms. Use m-ColiBlue24® broth to accurately monitor and evaluate total coliform and E. coli in drinking water; wastewater; bottled water; beverages; surface, ground, and well water; and ultrapure, chemical processing and pharmaceutical processing waters. With ready-to-use m-ColiBlue24® Broth, you can:

Supplier: Caron Products

Caron Environmental Test Chambers precisely control temperature and humidity conditions. Key standard features include a touchscreen user interface, built-in preventative maintenance, gVapor steam-free precision controlled humidity, gROD Refrigeration on Demand control system, a tool-less removable interior, sliding shelves, a horizontal airflow system that generates gentle and evenly distributed airflow across all shelf locations, heated triple-pane glass door (on 708 and 934 l models).

Supplier: Labconco

CentriVap™ Pro benchtop vacuum concentrators are designed to rapidly concentrate multiple small samples using centrifugal force, vacuum and heat.

Supplier: Elma

The dependable, state-of-the-art 37 kHz Elmasonic E Plus series includes 9 tank sizes with simple, user-friendly operation. Samples are rapidly mixed, dispersed, dissolved, or homogenized in Dynamic mode, while Eco mode offers quiet gentle cleaning at reduced power.

Supplier: NABERTHERM INC.

These weighing furnaces with integrated precision scale and software, were designed especially for determining combustion loss in the laboratory. The determination of combustion loss is necessary, for instance, when analyzing sludges. Combustion loss is defined as the difference between the initial total mass and the combustion residue. During the process the software supplied records both temperature and weight loss.

Supplier: Thermo Scientific

The Thermo Scientific Megafuge™ 8 centrifuge provides exceptional capacity in a compact design with a smart, simple interface and the flexibility to support both clinical and research applications. The centrifuge fits in to space, with its compact footprint, and to applications, accomodating multi-laboratory settings.

Supplier: Avantor Fluid Handling

Networking drives offer either native EtherNet/IP™ or Profibus communication-no additional hardware or firmware required.