30456 Results for: "Reagents"

Supplier: Prosci

IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL-6 appears to interact with IL2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass.

Supplier: RIGAKU REAGENTS MS

FILAMENT AP5S POINT FOCUS SET 3

Supplier: Promega Corporation

The Kinase-Glo Luminescent Kinase Assays are homogeneous non-radioactive systems for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction.

Supplier: GILSON, INC.

PIPETMAX is an automated pipetting solution for the efficient processing of high-throughput biological assays. It will help you to improve on the accuracy, reproducibility, and consistency among all samples processed. Unlike traditional automation platforms, PIPETMAX comes in a benchtop size that easily fits into any laboratory.

Supplier: Promega Corporation

NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL1.1[Nluc] and pNL1.2[NlucP] Vectors are used to clone putative promoter regions to express the bright NanoLuc(R) luciferase.

Supplier: RIGAKU REAGENTS MS

BLOCK PLATE 96WELL MATRIX WIZARD CRYO1-2

Supplier: Promega Corporation

NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL1.1[Nluc] and pNL1.2[NlucP] Vectors are used to clone putative promoter regions to express the bright NanoLuc(R) luciferase.

Supplier: MP Biomedicals

Ficin is a purified ficin preparation which is extracted from the latex of the fig tree Ficus glabrata. Ficin is classified as a thiol protease. Ficin hydrolyses the peptide bonds where the carbonyl group is from phenylalanine or tyrosine. When used in conjunction with other plants proteases, papain or bromelain, a synergistic effect may be observed. Immobilized Ficin was specifically designed for cleavage of mouse IgG1 into F(ab')2 or Fab fragments.The immobilization of ficin enhances stability against denaturation, heat and autolysis. Immobilization also eliminates any potential for antibody-enzyme adducts that cause continued sample digestion.

Supplier: Promega Corporation

NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL3.1[Nluc/minP] and pNL3.2[NlucP/minP] Vectors offer a minimal promoter for cloning response elements of interest.

Supplier: RIGAKU REAGENTS MS

ZINC CHLORIDE 1M 100ML

Supplier: RIGAKU REAGENTS MS

AMMONIUM SULFATE 3.5M 100 ML

Supplier: MP Biomedicals

Adenosine is a purine nucleoside comprising a molecule of adenine attached to a ribose sugar molecule moiety via a β-N9-glycosidic bond. It plays an important role in energy transfer. It is an endogenous purine nucleoside that modulates physiological process wherein cellular signaling by adenosine occurs through four known adenosine receptor subtypes (A1, A2A, A2B, and A3) Adenosine is an anti-inflammatory agent at the A(2A) receptor. Cardioprotective effects may relate to activation of A1 adenosine receptors. Endogenous neurotransmitter at adenosine receptors. Cardioprotective effects may relate to activation of A1 adenosine receptors. The antiplatelet and anti−inflammatory actions of adenosine appear to be mediated via the A2 adenosine receptor. In contrast, adenosine appears to be a pro-inflammatory mediator in asthma and chronic obstructive pulmonary disease (COPD). Adenosine is used in topical treatment of foot wounds in diabetes mellitus and also used for the treatment or prevention of cardiac arrhythmias (irregular heartbeat).It is used to cause dilation of the blood vessels. Physical Appearance: White powder Optical Rotation: -68.0° to -72.0° (c=2, NaOH 1:20) Heavy Metals: ≤0.001% Loss on Drying: ≤0.5% Residue on Ignition: ≤0.1% Storage Temp. : -20 °C

Supplier: Promega Corporation

The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

Supplier: MP Biomedicals

Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein. When adsorbed to the culture surface, poly-lysine increases the number of positively charged sites available for cell binding. A compromise between the easier to use lower molecular weight products and the extremely viscous higher molecular weights would be the products in the range of 70,000-150,000.
It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. Both the D- and L- form of the poly-lysine can be used as a coating substrate since poly-lysine is a nonspecific attachment factor for cells; however, certain cells can digest poly-lysine. In this case, poly-D-lysine should be used as the attachment factor so that the cells are not disrupted by excessive uptake of L-lysine.
Other uses for poly-lysine have been reported as well:
• Conjugation to methotrexate for increased drug transport.
• Microencapsulation of islets.
• Use in simple reproducible procedure for chromosomal preparations from a variety of tissues.
• Immobilation of living cells in biocompatible semipermeable microcapsules.
• In the preparation of polycationic beads.
• Conjugation to albumin and horseradish peroxidase to enhance cellular uptake.
Poly-D-lysine hydrobromide is a synthetic amino acid that enhances cell adhesion to solid substrates. It has additionally been shown to eliminate prion proteins from infected cells.

Supplier: MP Biomedicals

This colourless liquid is the simplest organic nitrile (hydrogen cyanide is a simpler nitrile, but the cyanide anion is not classed as organic). It is produced mainly as a byproduct of acrylonitrile manufacture.
Acetonitrile is widely used as a polar aprotic solvent in organic synthesis and in the purification of butadiene in refineries.
This solvent is often the preferred choice as a mobile phase in HPLC for a variety of reasons. Not only is it miscible with water and with many other organic solvents, but it also has a lower viscosity and produces less pressure in the column than other solvents such as Methanol. The inherent low absorbance of Acetonitrile (especially HPLC grade) makes it the preferred solvent for the mobile phase of HPLC due to the reduction in the amount of noise during UV detection. Overall, Acetonitrile has many unique properties as an organic mobile phase that often result in better chromatographic peaks.
Acetonitrile also plays an important role as the main solvent used in the manufacture of DNA oligonucleotides from monomers.
Room Temperature, store under nitrogen.

Supplier: RIGAKU REAGENTS MS

2-PROPANOL 100% V V 250 ML

Supplier: Rigaku Reagents

EBS-250-HEPES68 PH6.8 250ML

Supplier: REAGENTS DIRECT MS

CHIR99021 SELECTIVE INHIBITOR 2 MG

Supplier: Promega Corporation

NanoLuc (Nluc) luciferase is a 19.1kDa luminescent reporter enzyme that is about 100-fold brighter than either firefly or Renilla luciferase. Use the pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] Vector to measure changes in the levels of NF-kappaB in cells.

Supplier: Promega Corporation

The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

Supplier: Prosci

IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Supplier: MP Biomedicals

Natural tomato lycopene is a mixture of isomers, primarily (all-E), but also some (5-Z) and two lesser and unidentified cis isomers, all of which are difficult to resolve by LC. Lycopene is an open chain unsaturated carotenoid that gives tomatoes, guava, rose hip, watermelon and pink grapefruit its red color. Studies show that lycopene intake is significantly associated with lower risk of lung, colon and stomach carcinogenesis. It is an antioxidant.
Isolated from commercially-grown tomatoes (L. esculentum) and once recrystallized and hexane washed.
Antioxidant micronutrient of tomatoes associated with decreased risk for cancer and cardiovascular disease. Enhances gap junction communication between cells via upregulation of connexin 43 and reduces proliferation of cancer cells in culture. Inhibits cholesterol synthesis and enhances low-density lipoprotein degradation. In vitro, lycopene is a powerful carotenoid quencher of singlet oxygen, being 100 times more efficient in test tube studies of singlet-oxygen quenching action than vitamin E, which in turn has 125 times the quenching action of glutathione (water soluble). Lycopene metabolite apo-10'-lycopenal, or ALA, may have an important role in the metabolism of hepatic lipids, and may prevent build up.
-20 °C, protect from light, store under nitrogen.

Supplier: Biosensis

Fluoro-Jade B stains all degenerating neurons regardless of specific insult or mechanism of cell death. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.

Supplier: RIGAKU REAGENTS MS

SALT SCREEN WIZARD PEG ION 48 PEG 8K/10K

Supplier: Promega Corporation

The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

Supplier: REAGENTS DIRECT MS

INHIBITOR MEK 99.7% HPLC 2 MG

Supplier: REAGENTS INC MS

SOLUTION SODIUM LAURYL SULFATE 0.004N 4L

Supplier: RIGAKU REAGENTS MS

MIB BUFFER 1M PH 9.0 100ML

Supplier: Rigaku Reagents

SODIUM HYDROXIDE PH6.0 1M 100ML/VOLUME

Supplier: Molecular Devices

The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes