785 Results for: "Proteomics"

Supplier: Invitrogen

Thermo Scientific Pierce LC-SDA (NHS-LC-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 12.5Å spacer arm separates the two photoreactive groups.

Supplier: Invitrogen

Thermo Scientific Krypton Fluorescent Protein Stain enables sensitive visualization with fluorescence imagers (520nm excitation; 580nm emission) of proteins separated in polyacrylamide gels or transferred to membranes.

Supplier: MP Biomedicals

SC-Ura is a single dropout formulation of Synthetic Complete Supplement Mixture (SC) of amino acids for S. cerevisiae growth media.

Supplier: MP Biomedicals

CHAPS is a nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents.

Supplier: Invitrogen

Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Supplier: Cube Biotech

Glyco-DIBMA is a modified version of DIBMA with a sugar side-chain added to the polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins. After solving the Glyco-DIBMA + buffer powder in water, the product is ready-to-use. It can primarily be used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein of interest, the protein can be purified using affinity chromatography. We recommend the Rho1D4-tag for membrane protein purification and offer matching products for this purpose. DIBMA / Nanodisc Screening: Each membrane protein may perform better or differently with certain synthetic polymers. This is due to the characteristic composition of phospholipids surrounding the membrane proteins. Therefore a screening process for the correct DIBMA/Polymer is required in before. For this purpose, we offer our synthetic nanodisc screening kit MAXI which contains DIBMA, AASTY, and SMA.

Supplier: Cube Biotech

AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-50 - are highly-alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-50 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-50 show higher thermodynamic stability.

Every membrane protein solubilization needs to undergo a screening process in before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process, we offer a handy Screening Kit for AASTYs to test them all.

We recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Supplier: Thermo Scientific

The Thermo Scientific Megafuge™ 8 centrifuge provides exceptional capacity in a compact design with a smart, simple interface and the flexibility to support both clinical and research applications. The centrifuge fits in to space, with its compact footprint, and to applications, accomodating multi-laboratory settings.

Supplier: Invitrogen

Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.

Supplier: MP Biomedicals

Acrylamide is a monomer used in a variety of synthetic processes to form polymers and copolymers. It polymerizes in the presence of free radicals in aqueous solutions. It is usually used to prepare polyacrylamide gels for electrophoresis separation of biomolecules. Common reaction initiators are riboflavin and ammonium persulfate; varying the ratio of acrylamide to crosslinking agent permits the formation of a gel with predictable average pore size and texture. Acrylamide tends to hydrolyze under acidic or basic conditions to form acrylic acid. Polyacrylamide plastics have numerous commercial applications, due in part to the tendency of polyacrylamide to adsorb many times its own mass in water. Acrylic acid and any ionic impurities in the acrylamide can have significant effects on the performance of the acrylamide gel formed, since the voltage across the gel is affected by the ionic charge of the gel and usage buffers.
Store at Room Temperature (15-30 °C), Dessicated.

Supplier: Invitrogen

Thermo Scientific Pierce Premium Grade EDC is our highest quality formulation of this popular carbodiimide crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Supplier: ARBOR ASSAYS MS

The BCA Protein Dual Range Colorimetric Detection Kit from Arbor Assays offers sensitive, species-independent measurement of total protein concentrations in serum, plasma, urine, cell lysates, and tissue homogenates. With flexible protocols for regular (6.65 µg/ml sensitivity) and high sensitivity (1.68 µg/ml) formats, the assay accommodates a wide dynamic range from 6.25 to 1000 µg/ml. The BCA chemistry produces a purple product measurable at 560 nm, with minimal sample handling and broad matrix tolerance. This kit is ideal for applications in biochemistry, molecular biology, and clinical research, offering reliable, quantitative protein measurement with a simple 2 hour protocol.

Supplier: Invitrogen

Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.

Supplier: MP Biomedicals

Deoxycholic Acid Sodium Salt Monohydrate is an anionic biological detergent for the solubilization of membrane-bound proteins.

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-SMCC, No-Weigh Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).

Supplier: Promega Corporation

The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.

Supplier: Promega Corporation

The TNT Coupled Wheat Germ Extract Systems are one-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.

Supplier: MP Biomedicals

Pure Canavalia ensiformis lectin (Con A) from Jackbean.

Supplier: Invitrogen

Thermo Scientific EZ-Link Sulfo-NHS-LC-LC-Biotin enables simple and efficient biotin labeling of antibodies, proteins, and any other primary amine–containing macromolecules. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents.

Supplier: MP Biomedicals

Pure Canavalia ensiformis lectin (Con A) from Jackbean. Isolated by affinity chromatography on cross-linked dextran. Con A exists as a dimer below pH 5.0 and and at pH >7 it exists as a tetramer. Con-A is not a glycoprotein. The monomeric molecular weight of Con-A is 25500. Con-A does not contain cysteine residues. Unlike most other lectins, Con-A is a metalloprotein and requires a transition metal ion, such as manganese, plus calcium ions for binding.

Supplier: Promega Corporation

The HaloLink Protein Array System combines the innovative HaloTag technology, surface engineering and cell-free protein expression systems to enable parallel analysis of multiple protein:protein, protein:drug or protein:nucleic acid interactions.

Supplier: MP Biomedicals

Cholic acid is a biochemical solubilizing agent (a non-ionic, non-denaturing detergent). Occurs inconjugation with glycine or taurine in bile of most vertebrates.

Supplier: Cube Biotech

Each membrane protein has its native lipid environment. Therefore, each lipid environment is unique to each membrane protein. Moreover, it is this lipid environment that synthetic polymers like AASTYs interact with. Therefore, one needs a broader selection of polymers since there is not one AASTY that fits each phospholipid composition. To address this question, AASTYs come in three different ratios of acrylic acid to styrene. The ratios differ in hydrophilic property of AASTY, which results in different affinities to varying membrane lipid compositions. The second feature to screen for when it comes to AASTY is the size of the resulting nanodiscs. One of AASTY's most excellent features, compared to SMA or DIBMA, is that its nanodiscs are pretty uniform in size. Therefore, the correct fitting nanodisc size must be determined before.

Supplier: MP Biomedicals

Urea is a prinicipal protein metabolite end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle and the major product for the removal of free ammonia (NH4+) in vivo.

Supplier: MP Biomedicals

Storage: Room Temperature, desiccate, store under nitrogen.
Ferrous sulfate heptahydrate is a reagent used in the manufacturing of iron compounds, in electroplating baths, aluminium etching, process engraving and lithography, and in redox polymerisation. It is used as a reducing agent in chemical processes, leather dyes and writing inks. Also, it is a component of weed killers, wood preservatives and water treatment processes. Used In prevention of chlorosis in plants and therapeutically as hematinic. In veterinary medicine, it is used as an astringent to combat iron deficiency. Ferrous sulfate heptahydrate is used in the determination of phosphorus as per the method of Taussky and Shorr. Ferrous sulfate heptahydrate is used in cell culture applications generally bound to transferrin, citrate or other iron transport molecules.

Supplier: Molecular Devices

The GenePix® 4300A and GenePix® 4400A Scanners from Molecular Devices offer maximum imaging quality with optimal resolution in highly configurable platforms.

Supplier: MP Biomedicals

Trichloroacetic acid is an acetic acid analogue commonly used to precipitate proteins, DNA and RNA. In the presence of SDS Trichloroacetic acid will precipitate proteins which can then be quantified by the Lowry method. The compound has also found uses as a decalcifier and fixative in microscopy, in protein sequencing, detecting albumin and organic synthesis.
Trichloroacetic acid is used in protein precipitation; has been used to determine protein concentration by quantitative precipitation. It is also used as a decalcifier and fixative in microscopy. A protocol for the precipitation of nucleic acids can be found in Molecular Cloning. Rats exposed to chronic sublethal amounts of trichloroacetic acid displayed an increase in serum bilirubin with a decrease in hematological proteins and cholesterols along with significant decreases in red blood corpuscles, mean cell volume, mean corpuscular hemoglobin, hemoglobin and hematocrit. In mice the production of liver tumors was associated to Trichloroacetic acid, which is also a known mouse hepatocarinogen. On the guinea pig trichloroacetic acid was observed to be a mild alergen on the skin.
+4 °C

Supplier: Cytiva

Ettan™ IPGphor™ 3 is a fully integrated isoelectric focusing (IEF) system optimized to deliver high throughput, speed, reproducibility and high protein-loading capacity.

Supplier: Cube Biotech

Membrane proteins and their composition are as versatile as the whole cell membrane itself. For this reason, it is impossible to predict with 100% accuracy which SMALP product is best suited for the solubilization and subsequent preparation of your membrane protein of interest. We offer this SMALP Screening Kit that consists of our four individual SMAs. This way, you can conveniently screen for the best SMA for your protein before scaling up your assay. In case you would like to extend your polymer screening beyond the reaches of SMA, we offer our Synthetic Nanodisc Screening Kit, which covers both SMA and DIBMA.