784 Results for: "Proteomics"

Supplier: MP Biomedicals

2XYT-Broth is a nutritional rich medium providing nitrogen and growth factors optimized for the growth of recombinant E. coli strains infected with the M13 bacteriophage or other filamentous single stranded DNA bacteriophages thus allowing the bacteriophage to reproduce in large quantities without exhausting the host cells.

Supplier: AGILENT TECHNOLOGIES, INC (CSD)

ZORBAX 300Å StableBond columns are an ideal choice for reproducible separations of proteins and peptides available in four different bonded phases: C18, C8, C3, and Diphenyl

Supplier: Invitrogen

Thermo Scientific Pierce EMCH is a mid-length, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Supplier: Cube Biotech

With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.

Supplier: Cube Biotech

The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: Invitrogen

Thermo Scientific Pierce SDA (NHS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 3.9Å spacer arm separates the two photoreactive groups.

Supplier: MP Biomedicals

Sterile YT media formulations are optimized for the growth and maintenance of M13 phage or other filamentous ssDNA bacteriophages.

Supplier: MP Biomedicals

Rich Medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.

Supplier: Thermo Scientific

The Thermo Scientific Megafuge™ 8 centrifuge provides exceptional capacity in a compact design with a smart, simple interface and the flexibility to support both clinical and research applications. The centrifuge fits in to space, with its compact footprint, and to applications, accomodating multi-laboratory settings.

Supplier: Invitrogen

Thermo Scientific EZ-Link Sulfo-NHS-SS-Biotin is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

Supplier: Invitrogen

Thermo Scientific Pierce SDAD (NHS-SS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.

Supplier: MilliporeSigma

SMCC, Water-Soluble, a water-soluble derivative of SMCC.

Supplier: MICROBIOLOGICS, INC.

Applications include Enumeration Methods, Detection Methods, Demonstration of Capability (DOC), Media QC, and Validation/Verification.

Supplier: Invitrogen

Thermo Scientific Pierce Premium Grade TCEP-HCl is our highest quality formulation of this disulfide reducing agent, specially characterized for applications where product integrity and risk minimization are paramount.

Supplier: Promega Corporation

The E. coli T7 S30 Extract System for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or lambda vectors containing a T7 promoter.

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-LC-SDA (Sulfo-NHS-LC-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 12.5Å spacer arm separates the two photoreactive groups.

Supplier: Promega Corporation

The TNT Coupled Wheat Germ Extract Systems are one-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

Supplier: Invitrogen

Thermo Scientific EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Supplier: Invitrogen

Thermo Scientific Pierce Premium Grade Sulfo-NHS-LC-Biotin is our highest quality formulation of this popular amine-reactive biotinylation reagent, specially characterized for applications where product integrity and risk minimization are paramount.

Supplier: Invitrogen

Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the  amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.

Supplier: Invitrogen

Thermo Scientific Pierce LC-SPDP is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-SDA (Sulfo-NHS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 3.9Å spacer arm separates the two photoreactive groups.

Supplier: Invitrogen

Thermo Scientific Pierce Premium Grade Sulfo-NHS is our highest quality formulation of this N-hydroxysuccinimide chemical modification reagent, specially characterized for applications where product integrity and risk minimization are paramount.

Supplier: Invitrogen

Thermo Scientific CHAPS is a zwitterionic detergent that is especially well suited for protecting the native state of proteins.

Supplier: MP Biomedicals

Terrific broth II is a nutritionally rich medium for the growth of E. coli cells containing yeast extract and tryptone in elevated concentrations as well as sodium chloride and vitamins for a luxurious growth.

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.

Supplier: Invitrogen

Thermo Scientific Pierce LC-SDA (NHS-LC-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 12.5Å spacer arm separates the two photoreactive groups.

Supplier: MP Biomedicals

SC-Ura is a single dropout formulation of Synthetic Complete Supplement Mixture (SC) of amino acids for S. cerevisiae growth media.

Supplier: Invitrogen

Thermo Scientific Krypton Fluorescent Protein Stain enables sensitive visualization with fluorescence imagers (520nm excitation; 580nm emission) of proteins separated in polyacrylamide gels or transferred to membranes.