785 Results for: "Proteomics"

Supplier: MP Biomedicals

X-Gal, is a Beta-galactosidase substrate, Ultra pure, Purity: >99%, CAS Number: 7240-90-6, Molecular Formula: C14H15BrClNO6, Molecular Weight: 408.6, Synonyms: 5-Bromo-4-chloro-3-indolyl B-D-galactoside, BCIG, Color: White, Form: Powder, Size: 1.0 g

Supplier: Honeywell Research Chemicals

2-Propanol (Isopropyl alcohol, Isopropanol, IPA) is a colorless solvent and is the simplest example of a secondary alcohol. It is moderately polar and has a boiling point of 82 °C. 2-Propanol dissolves a wide range of non-polar compounds and it evaporates quickly. Thus, it is used widely as a cleaning fluid, especially for dissolving oils. Suitable for LC-MS and cleaning LC-MS systems.

Supplier: Invitrogen

Thermo Scientific Pierce LC-SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long cyclohexane-stabilized spacer arm (16.2 angstroms).

Supplier: Invitrogen

Thermo Scientific Pierce DTBP is dimethyl 3,3'-dithiobispropionimidate, a crosslinker that contains amine-reactive imidoester groups around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

Supplier: Promega Corporation

Non-Radioactive Translation Detection Systems for colorimetric or chemiluminescent detection of proteins synthesized in vitro. Biotinylated proteins are detected using Streptavidin-Alkaline Phosphatase or Streptavidin-HRP.

Supplier: Cube Biotech

IPTG is a modified sugar that can be used to induce protein expression under the control of the lacUV promotor. Our IPTG is of highest quality and purity. Like allolactose, IPTG binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner, thereby allowing the transcription of genes in the lac operon. But unlike allolactose, the sulfur (S) atom creates a chemical bond which is non-hydrolyzable by the cell, preventing the cell from metabolizing or degrading the inducer. The concentration of IPTG therefore remains constant and the expression of lac p/o-controlled genes would not be inhibited during the experiment.

Supplier: Invitrogen

Thermo Scientific EZ-Link Hydrazide-LC-Biotin is a mid-length, simple, hydrazide-activated biotinylation reagent for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes.

Supplier: MP Biomedicals

Glycerol is a simple polyol compound which is a viscous liquid that is widely used in pharmaceutical formulations. It has three hydroxyl groups that are responsible for its solubility in water and its hygroscopic nature.It is a trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, sweetening agent and is of low toxicity.

Supplier: MP Biomedicals

MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. It is a structural analog to MES. However, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4.
Store at Room Temperature (15-30 °C)

Supplier: MP Biomedicals

Tunicamycin is a mixture of Tunicamycins A, B, C and D. It is a nucleoside antibiotic active in vitro against Gram-positive bacteria, yeasts, fungi, and viruses.

Supplier: Cube Biotech

With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.

Supplier: Invitrogen

Thermo Scientific Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm.

Supplier: AAT Bioquest

Compared to SMCC and sulfo-SMCC, SMCC Plus has enhanced water solubility and crosslinking efficiency.

Supplier: MICROBIOLOGICS, INC.

Applications include Enumeration Methods, Detection Methods, Demonstration of Capability (DOC), Media QC, and Validation/Verification.

Supplier: MP Biomedicals

Rich medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.

Supplier: MP Biomedicals

CSM-Ura is a single dropout time tested formulation of complete supplement mixture (CSM) of amino acids for S. cerevisiae growth media.

Supplier: Invitrogen

Thermo Scientific Pierce SDA (NHS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 3.9Å spacer arm separates the two photoreactive groups.

Supplier: AGILENT TECHNOLOGIES, INC (CSD)

ZORBAX 300Å StableBond columns are an ideal choice for reproducible separations of proteins and peptides available in four different bonded phases: C18, C8, C3, and Diphenyl

Supplier: MP Biomedicals

Sterile YT media formulations are optimized for the growth and maintenance of M13 phage or other filamentous ssDNA bacteriophages.

Supplier: MP Biomedicals

Rich Medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.

Supplier: Cube Biotech

The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: Invitrogen

Thermo Scientific Pierce EMCH is a mid-length, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Supplier: Cube Biotech

With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.

Supplier: Boster Biological Technology

Rabbit IgG polyclonal antibody for Pyruvate kinase PKM(PKM) detection. Tested with WB, IHC-P in Human.

Supplier: Promega Corporation

TNT Coupled Reticulocyte Lysate Systems are single-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

Supplier: MP Biomedicals

Rich medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.

Supplier: MP Biomedicals

CSM-Leu is a Single dropout formulation of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media.

Supplier: MP Biomedicals

DOB is a component of synthetic defined media (SD), which is used for growth of S. cerevisiae.

Supplier: MP Biomedicals

Luria-Bertani, Rich Medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.

Supplier: MP Biomedicals

2XYT-Broth is a nutritional rich medium providing nitrogen and growth factors optimized for the growth of recombinant E. coli strains infected with the M13 bacteriophage or other filamentous single stranded DNA bacteriophages thus allowing the bacteriophage to reproduce in large quantities without exhausting the host cells.