784 Results for: "Proteomics"
DMP (Dimethyl pimelimidate dihydrochloride), Pierce™
Supplier: Invitrogen
Thermo Scientific Pierce DMP is dimethyl pimelimidate, a membrane-permeable crosslinker that contains an amine-reactive imidoester group at each end of a 7-atom spacer arm.
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TNT SP6 Coupled Reticulocyte Lysate System, Promega
Supplier: Promega Corporation
TNT Coupled Reticulocyte Lysate Systems are single-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.
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LB Agar, Lennox Capsules, MP Biomedicals
Supplier: MP Biomedicals
Rich medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.
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Fluorescamine, white powder
Supplier: MP Biomedicals
Fluorescamine is a non-fluorescent reagent that reacts readily with primary amino groups in amino acids and peptides to form highly fluorescent derivatives, whereas the reagent and its degradation products are non-fluorescent. No significant fluorescence with ammonia. Low background due to hydrolysis.
an plasma and urine and sulfonamides in honey by HPLC with fluorescence detection.
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TNT SP6 High-Yield Wheat Germ Protein Expression System, Promega
Supplier: Promega Corporation
TNT SP6 High-Yield Wheat Germ Protein Expression System, based on an optimized wheat germ extract, is a single-tube, coupled transcription/translation system designed to express proteins in only two hours.
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TNT T7 Coupled Reticulocyte Lysate System, Promega
Supplier: Promega Corporation
TNT Coupled Reticulocyte Lysate Systems are single-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.
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Sulfo-SMCC (3-Sulfo-N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate sodium salt), OneQuant™
Supplier: G-Biosciences
Ideal for enzyme-antibody crosslinking.
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LB Agar Medium, MP Biomedicals
Supplier: MP Biomedicals
Rich medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.
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DIBMA glucosamine in HEPES, PureCube for protein solubilisation
Supplier: Cube Biotech
With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.
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E. coli S30 Extract System for Circular DNA, 30 Reactions, Promega
Supplier: Promega Corporation
E. coli S30 Extract for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or lambda vectors.
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Sulfo-NHS Biotin, EZ-Link™ No-Weigh™ Format
Supplier: Invitrogen
Thermo Scientific EZ-Link Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
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VWR® Blotting Paper – High-Performance Absorbent Sheets
Supplier: VWR International
VWR® blotting papers are high-quality, absorbent laboratory sheets designed for capillary transfer, buffer wicking, and surface drying applications. Manufactured from 100% pure cotton fiber and processed with ultrapure water, these papers offer superior absorbency, uniform thickness, and clean transfer properties for nucleic acid and protein blotting workflows.
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Sulfo-NHS (N-Hydroxysulfosuccinimide sodium salt)
Supplier: G-Biosciences
G-Biosciences' Sulfo-NHS reacts primarily with primary amines to convert carboxyl groups to amine reactive sulfo-NHS esters
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Biocytin, EZ-Link™
Supplier: Invitrogen
Thermo Scientific EZ-Link Biocytin is a simple variant of biotin that contains a primary amine in its valeric acid chain, which provides the essential backbone for construction of certain long-chain and trifunctional biotinylation reagents.
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Desthiobiotin NHS ester, EZ-Link™, Pierce™
Supplier: Invitrogen
Thermo Scientific EZ-Link NHS-Desthiobiotin is a short, membrane permeable, amine-reactive labeling reagent whose biotin-like group is elutable from streptavidin, making it ideal for intracellular protein labeling and purification.
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5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB), Pierce™
Supplier: Invitrogen
Thermo Scientific Pierce Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.
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Sulfo-SANPAH (Sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate), Pierce™
Supplier: Invitrogen
Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.
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Anti-S100A14 Rabbit Polyclonal Antibody
Supplier: Cloud-Clone
Polyclonal Antibody to S100 Calcium Binding Protein A14 (S100A14), derived from recombinant S100A14 (Met1~Ala91), is reactive with Human/Mouse/Pig.
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Transcend tRNA, 30 µl, Promega
Supplier: Promega Corporation
Non-Radioactive Translation Detection Systems for colorimetric or chemiluminescent detection of proteins synthesized in vitro. Biotinylated proteins are detected using Streptavidin-Alkaline Phosphatase or Streptavidin-HRP.
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Sulfo-SMCC (3-Sulfo-N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate sodium salt)
Supplier: G-Biosciences
Ideal for enzyme-antibody crosslinking.
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A-Line Vials, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES, INC (CSD)
These A-Line certified vials are constructed from Type 1 borosilicate glass, with low metal content, to protect samples from destabilizing or leaching. They are designed to provide the ultimate solution for handling precious samples.
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Bolton-Hunter reagent (Sulfo-SHPP), Pierce™
Supplier: Invitrogen
Sulfo-SHPP, sulfosuccinimidyl-3 -(4-hydroxypheynyl) propionate, also known as water-soluble Bolton-Hunter reagent, conjugates tyrosine-like functional groups to primary amines to increase the number of tyrosyl groups that can be iodinated by iodine-125 labeling procedures.
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Bis-Tris Mini Protein Gels, GenScript®
Supplier: Genscript
GenScript's Bis-Tris gels are high-performing polyacrylamide gels designed to separate a wide range of protein sizes by electrophoresis. The gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis and increases gel stability. Furthermore, the neutral pH which minimizes protein modification when compared to Tris-glycine gels.
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M9 Minimal Salts (Maniatis Formulation) Powder, MP Biomedicals
Supplier: MP Biomedicals
M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E
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di-Sodium succinate hexahydrate ≥98%, white crystalline powder
Supplier: MP Biomedicals
Soluble in water (100 mg/mL - clear, colorless solution).
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EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)], Pierce™
Supplier: Invitrogen
Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.
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Sulfo-NHS (N-Hydroxysulfosuccinimide sodium salt), Pierce™
Supplier: Invitrogen
Pierce™ Sulfo-NHS (N-hydroxysulfosuccinimide) is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.
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TNT® T3 Coupled Reticulocyte Lysate System, 40 Reactions, Promega
Supplier: Promega Corporation
TNT Coupled Reticulocyte Lysate Systems are single-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.
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X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) ≥99% (by TLC), white powder, Ultrapure
Supplier: MP Biomedicals
X-Gal, is a Beta-galactosidase substrate, Ultra pure, Purity: >99%, CAS Number: 7240-90-6, Molecular Formula: C14H15BrClNO6, Molecular Weight: 408.6, Synonyms: 5-Bromo-4-chloro-3-indolyl B-D-galactoside, BCIG, Color: White, Form: Powder, Size: 1.0 g
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SMALP 140
Supplier: Cube Biotech
The SMALP products have been developed for biomedical purposes, in particular, for the solubilization of (biological) lipid membranes and isolation & purification of integral membrane proteins.
After solving the SMA + buffer powder in water, the product is ready-to-use. It can primarily used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.