784 Results for: "Proteomics"

Supplier: New England Biolabs (NEB)

Sodium Orthovanadate (Vanadate, Na3VO4) is a commonly used general inhibitor for protein phosphotyrosyl phosphatases (PTPs). It is a competitive inhibitor. The inhibition by Vanadate is completely reversible upon the addition of EDTA or by dilution. Vanadate is activated for maximal inhibition of PTPs following the procedure described by J.A.Gordon. The PTP activities are conveniently separable from the protein phosphoseryl and phosphothreonyl phosphatase (PSP) activities inhibited by Fluoride and EDTA. Routinely Vanadate is used to preserve the protein tyrosyl phosphorylation state in cells, cell lysates, and protein tyrosine kinase assays.

Supplier: Invitrogen

Thermo Scientific Pierce AMAS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a very short spacer arm (4.4 angstroms).

Supplier: Invitrogen

Thermo Scientific Pierce BMPS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (5.9 Å).

Supplier: MP Biomedicals

Pepstatin A is a reversible inhibitor of aspartic proteases, Inhibitor for pepsin, renin, cathepsin D, cathepsin G, and other acid proteases

Supplier: Invitrogen

Thermo Scientific Pierce 8M Guanidine-HCl Solution is an accurately formulated, ready-to-use solution of guanidine hydrochloride, that can be easily diluted and pH-adjusted to any concentration below 8M.

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-EGS is a water-soluble crosslinker that contains amine-reactive sulfo-NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.

Supplier: Agilent Technologies

The Human 14 Multiple Affinity Removal Column is designed to chromatographically remove fourteen interfering high-abundant proteins from human plasma samples.

Supplier: G-Biosciences

G-Biosciences' InGel™ Silver provides a complete set of reagents for the in gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS)

Supplier: TCI America

[Coupling Agent for Peptides Synthesis] CAS Number: 25952-53-8 MDL Number: MFCD00012503 Molecular Formula: C8H17N3 Molecular Weight: 191.70 Purity/Analysis Method: 98.0% (T) Form: Crystal Melting point (°C): 115 Storage Temperature: 0-10°C

Supplier: BeanTown Chemical

CAS: 25952-53-8; EC No: 247-361-2; MDL No: MFCD00012503; RTECS: FF2200000 Crystalline/Powder; Molecular Formula: C8H17N3·HCl; MW: 191.70 Melting Point: 110-115° Moisture Sensitive

Supplier: Alkali Scientific

Experience minimal sample loss with Alkali Scientific™ Rainin LTS™ LiteTouch™ filter tips - low retention, ensuring accurate pipetting.

Supplier: Invitrogen

SATA (and SATP) are reagents for introducing protected sulfhydryls into proteins, peptides and other molecules. for covalent modification of primary amines and addition of a protected sulphhydryl group.

Supplier: Invitrogen

Thermo Scientific Pierce SMPB is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).

Supplier: AAT Bioquest

This PEG biotin-labeled amino acid has excellent solubility in DMF and other solvents used in SPPS.

Supplier: AAT Bioquest

The FMK peptides are potent, cell permeable, irreversible capase inhibitors.

Supplier: Promega Corporation

Wheat Germ Extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors) and is used for in vitro translation..

Supplier: MP Biomedicals

Soluble in water (100 mg/mL); practically insoluble in benzene, chloroform or ether.

Supplier: Invitrogen

Thermo Scientific Pierce Trifluoroacetic Acid (TFA), Sequencing grade is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

Supplier: AAT Bioquest

MM(PEG)24 is a methyl-terminated, polyethylene glycol compound (24 PEG units) activated with a maleimide group for covalent pegylation of sulfhydryls on biomolecules or assay surfaces.

Supplier: New England Biolabs (NEB)

Furin is a ubiquitous subtilisin-like proprotein convertase. It is the major processing enzyme of the secretory pathway and is localized in the trans-golgi network. Substrates of Furin include blood clotting factors, serum proteins and growth factor receptors such as the insulin-like growth factor receptor. The minimal cleavage site is Arg-X-X-Arg'. However, the enzyme prefers the site Arg-X-(Lys/Arg)-Arg'. An additional arginine at the P6 position appears to enhance cleavage. Furin is inhibited by EGTA, α1- Antitrypsin Portland and polyarginine compounds.

Supplier: Invitrogen

Thermo Scientific Octylthioglucoside is a low-MW nonionic detergent that is effective for cell lysis and nondenaturing protein solubilization.

Supplier: MP Biomedicals

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues

Supplier: Invitrogen

Thermo Scientific EZ-Link Hydrazide-Biotin is the shortest and simplest hydrazide-activated biotinylation reagent for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes.

Supplier: AAT Bioquest

Cu-free click reaction, i.e., the strain-promoted alkyne-azide cycloaddition (SPAAC) is a bioorthogonal reaction utilizing a pair of reagents, cyclooctynes and azides that exclusively and efficiently react with each other while remain inert to naturally occurring functional groups such as hydroxy, amino and thiol groups.

Supplier: Invitrogen

Thermo Scientific Pierce DTME is a mid-length, maleimide crosslinker for reversible covalent conjugation between sulfhydryl groups (e.g., protein or peptide cysteines) by reduction of the disulfide bond in the center of the spacer arm.

Supplier: Invitrogen

Thermo Scientific Pierce L-Photo-Methionine is an analog of L-methionine that can be incorporated into proteins during synthesis and then light-activated to covalently crosslink interacting proteins in cells.

Supplier: MilliporeSigma

Bifunctional cross-linker. The disulfide bond can be cleaved with 1% NaBH4, 50 mM DTT, or 100 mM β-mercaptoethanol. Has been used to immobilize proteins on supports containing amino groups.

Supplier: Invitrogen

Thermo Scientific Pierce MPBH is a long, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Supplier: Invitrogen

Thermo Scientific Pierce DMS is dimethyl suberimidate, a membrane-permeable crosslinker that contains an amine-reactive imidoester group at each end of an 8-atom spacer arm.

Supplier: AMPLYUS LLC

Examine the primary, secondary, and tertiary structures of fluorescent proteins.