296 Results for: "Protein molecular weight markers"

Supplier: Biosensis

Lysosomal Associated Membrane Protein 1 Monoclonal antibody, Clone: 5H6, Host: mouse, Reactivity: Human, Isotype: IgG1, Immunogen: Recombinant LAMP1 expressed and purified from E. Coli, Applications: WB, ICC, Flow

Supplier: MP Biomedicals

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature

Supplier: Cloud-Clone

This assay has high sensitivity and excellent specificity for detecting Mouse PCT (Procalcitonin). The assay range is from 0.156 to 10 pg/ml (Sandwich kit) with a sensitivity of 0.069 pg/ml. There is no detectable cross to reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

Supplier: Cloud-Clone

This assay has high sensitivity and excellent specificity for detecting Human PCT (Procalcitonin). The assay range is from 31.2 to 2,000 pg/ml (Sandwich kit) with a sensitivity of 12.4 pg/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

Supplier: Invitrogen

Immunoprecipitate using antibodies immobilized by their Fc-region glycosylation.

Supplier: Biotium

Calponin-1 Monoclonal antibody, Clone: CNN1/832, Host: Mouse, Species: Human, Rat, Isotype: IgG1, kappa, Conjugate: CF405S, Immunogen: Multiple isoelectric variants of calponin have been identified, 34kDa/29kDa, Synonyms: CNN1, Cnn1, Application: IF, IHC, Fc, Size: 100ul

Supplier: Cloud-Clone

This assay has high sensitivity and excellent specificity for detecting Human PCT (Procalcitonin). The assay range is from 6.86 to 5,000 pg/ml (Sandwich kit) with a sensitivity of 2.75 pg/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

Supplier: Cloud-Clone

This assay has high sensitivity and excellent specificity for detecting Human IgG1 (Immunoglobulin G1). The assay range is from 6.25 to 400 ng/ml (Sandwich kit) with a sensitivity of 2.83 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

Supplier: Cloud-Clone

This assay has high sensitivity and excellent specificity for detecting Mouse IgG1 (Immunoglobulin G1). The assay range is from 246.9 to 20000 ng/ml (Competitive kit) with a sensitivity of 89.9 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

Supplier: MP Biomedicals

Human chorionic gonadotropin (HCG), a polypeptide hormone produced by the human placenta, is composed of an alpha and a beta sub-unit. The alpha sub-unit is essentially identical to the alpha sub-units of the human pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), as well as to the alpha sub-unit of human thyroid-stimulating hormone (TSH). The beta sub-units of these hormones differ in amino acid sequence
The action of HCG is virtually identical to that of pituitary LH, although HCG appears to have a small degree of FSH activity as well. It stimulates production of gonadal steroid hormones by stimulating the interstitial cells (Leydig cells) of the testis to produce androgens and the corpus luteum of the ovary to produce progesterone. Androgen stimulation in the male leads to the development of secondary sex characteristics and may stimulate testicular descent is present. This descent is usually reversible when HCG is discontinued. During the normal menstrual cycle, LH participates with FSH in the development and maturation of the normal ovarian follicle, and the mid-cycle LH surge triggers ovulation. HCG can substitute for LH in this function

Supplier: Cloud-Clone

This assay has high sensitivity and excellent specificity for detecting Human PIIINP (Procollagen III N to Terminal Propeptide). The assay range is from 62.5 to 4,000 pg/ml (Sandwich kit) with a sensitivity of 20.6 pg/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

Supplier: Cloud-Clone

This assay has high sensitivity and excellent specificity for detecting Mouse PIIINP (Procollagen III N to Terminal Propeptide). The assay range is from 125 to 8000 pg/ml (Sandwich kit) with a sensitivity of 54 pg/ml. There is no detectable cross to reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

Supplier: Cloud-Clone

This assay has high sensitivity and excellent specificity for detecting Rat PIIINP (Procollagen III N to Terminal Propeptide). The assay range is from 31.2 to 2000 pg/ml (Sandwich kit) with a sensitivity of 12.1 pg/ml. There is no detectable cross to reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

Supplier: Cloud-Clone

This assay has high sensitivity and excellent specificity for detecting Human PIIINP (Procollagen III N to Terminal Propeptide). The assay range is from 4.94 to 400 ng/ml (Competitive kit) with a sensitivity of 2.13 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

Supplier: Biotium

Cytokeratin, LMW Monoclonal antibody, Clone: KRTL/1077, Host: Mouse, Species reactivity: Human, Rat, Isotype: IgG1, kappa, Conjugate: CF405S, Immunogen: Recom human KRT77 protein, Synonyms: K1B, KRT1B, K77, CK-1B, Application: IF, IHC, WB, FC, Size: 100uL

Supplier: BioVendor

Quality control test, Indirect ELISA – to determine titer of the antibody , SDS PAGE – to determine purity of the antibody, and BCA - to determine quantity of the antibody.

Supplier: DRG International

An enzyme immunoassay for the quantitative determination of myoglobin concentration in serum.

Supplier: Biotium

Cytokeratin, HMW Monoclonal antibody, Clone: KRTH/1076, Host: Mouse, Species reactivity: Human, Rat, Isotype: IgG1, kappa, Conjugate: CF405S, Immunogen: Recom human KRT76 protein, Synonyms: KRT2B, KRT2P, HUMCYT2A, Application: IF, WB, FC, Size: 500 uL

Supplier: Cloud-Clone

This assay has high sensitivity and excellent specificity for detecting Human PIIINP (Procollagen III N to Terminal Propeptide). The assay range is from 0.625 to 40 ng/ml (Sandwich kit) with a sensitivity of 0.241 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

Supplier: Cloud-Clone

Polyclonal antibody to Immunoglobulin G1 (IgG1), derived from native IgG1, is reactive with Human.

Supplier: Genetex

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki67 index and histologically low-grade tumours is strong.

Supplier: Cloud-Clone

Polyclonal Antibody to Procalcitonin (PCT), derived from recombinant PCT(Ala26~Asn141), is reactive with Human/Mouse/Pig.

Supplier: Cloud-Clone

FITC-Linked Polyclonal antibody to Immunoglobulin G1 (IgG1), derived from native IgG1, is reactive with Human.

Supplier: Genetex

Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. High levels of these enzymes are present in proliferating cells, and low levels are found in resting cells, due to multiple regulatory mechanisms that ensure exclusive expression of these enzymes in replicating cells. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis. The activities of enzymes such as TK are essential for the activation of several chemotherapeutically important nucleoside analogues that are administered as prodrugs.

Supplier: MP Biomedicals

Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The average chain of limit digest is a tetranucleotide. DNase I acts upon single chain DNA, and upon double-stranded DNA and chromatin. In the latter case, although histones restrict susceptibility to nuclease action, over a period of time nearly all chromatin DNA is acted upon. According to Mirsky and Silverman, this could result from the looseness of histone attachment to DNA. They found that lysine-rich histones more effectively block DNase access to DNA than arginine-rich histones. Billing and Bonner suggest that DNase attacks the histone-free strand of chromatin DNA. Schmidt, et. al.indicate that hydrolysis of the histone-free region of DNA strands accounts for the initial rapid action of the enzyme on chromatin. Bollum reports degradation of synthetic homopolymer complexes by DNase I. The intracellular functions of the enzyme are probably controlled by a DNase inhibitor, which according to Lazarides and Lindberg is actin.

Supplier: PROTEIN SIMPLE MS

MARKER MOLECULAR WEIGHT MAURICE CE-SDS