311 Results for: "Protein A Chromatography Resin"

Supplier: Cytiva

HiTrap IMAC FF is prepacked with IMAC Sepharose 6 Fast Flow. The resin is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Supplier: New England Biolabs (NEB)

The Anti-SNAP-tag Antibody (Polyclonal) can be used in Western blots with SNAP-tag and CLIP-tag proteins. Polyclonal antibodies are produced from the immunization of rabbit with purified recombinant SNAP-tag protein and affinity purified using Protein A Affinity Chromatography resin.

Supplier: Cytiva

HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Supplier: Genscript

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. The protein A is most commonly bound to a chromatography resin, used for production of antibody drugs or reagents. Protein A antibody can be used for quantification of protein A in sample foods or for evaluation of protein A resin. This protein A mAb is advantageous due to its IgG1 isotype, for mouse IgG1 has weak binding capacity to protein A.GenScript protein A Antibody [biotin], mAb, mouse is protein A antibody conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of protein A.

Supplier: Cytiva

The HiScreen MabSelect SuRe column uses protein A chromatography for antibody purification. This protein A column is prepacked with MabSelect SuRe affinity resin and is part of our process development platform. These packed columns are used for method optimization and parameter screening for capture of monoclonal antibodies.

Supplier: Cytiva

HiTrap IMAC HP is prepacked with IMAC Sepharose High Performance. The resin is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent high-resolution purification of polyhistidine tagged proteins.

Supplier: Genscript

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. The Protein A is most commonly bound to a chromatography resin, used for production of antibody drugs or reagents. Protein A antibody can be used for quantification of Protein A in drugs and foods or for evaluation of Protein A resin. This Protein A mAb is advantageous due to its IgG1 isotype, for mouse IgG1 has weak binding capacity to Protein A. GenScript Protein A Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified recombinant Protein A.

Supplier: Cytiva

HiTrap Capto MMC ImpRes is a ready-to-use column pre-packed with BioProcess Capto MMC ImpRes weak cation exchange multimodal resin for screening and small-scale protein purifications using ion exchange chromatography (IEX).

Supplier: Cytiva

HiTrap Capto adhere ImpRes is a ready-to-use column pre-packed with BioProcess Capto adhere ImpRes strong anion exchange multimodal resin for screening and small-scale protein purifications using ion exchange chromatography (IEX).

Supplier: Cytiva

These columns are prepacked with MabSelect™ PrismA protein A chromatographyn resin that has been improved with an optimised high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing efficient cleaning between purification runs. These columns are suitable for purifying antibodies with affinity chromatography in research and process development.

Supplier: Cytiva

These columns are prepacked with MabSelect™ PrismA protein A chromatographyn resin that has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing efficient cleaning between purification runs. These columns are suitable for purifying antibodies with affinity chromatography in research and process development.

Supplier: Cytiva

The Capto hydrophobic interaction chromatography (HIC) selection kit has a set of five 1 ml ready-to-use HiTrap™ columns for resin screening and small-scale protein purification. The HiTrap™ Capto HIC selection kit is useful in process development or research applications.

Supplier: Cytiva

The Untagged column package includes cation/anion exchange and size exclusion chromatography columns for purification of untagged/native proteins. The columns provided in the package are prepacked with Capto Q ImpRes and Capto SP ImpRes high-performance resins and can be used individually or in combination.

Supplier: Cytiva

Capto™ L is a bioprocessing chromatography resin for the capture of antibodies and antibody fragments. It combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein, L ligand, which has a strong affinity to the variable region of antibody kappa light chains. It is suitable for the capture of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

Supplier: Proteintech

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.

Supplier: Proteintech

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (Maltose binding protein) TAG in some expression systems.

Supplier: Cytiva

Capto™ adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules. Capto™ adhere ImpRes is designed to allow effective MAb polishing in the second or third step of a purification scheme downstream of the protein A capture step. Polishing can be performed in either bind and elute (binding) or flowthrough (non binding) modes.

Supplier: Invitrogen

Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.Easy-to-use—resin provided in single-use spin column formatImproved protein identifications—protein identifications increased by ≥50% when compared to unfractionated samplesReproducible—elution profiles and fractional resolution vary by less than 20%Optimized—robust procedure for maximal protein identification and peptide recovery while minimizing fractional overlapCompatible—reagents validated with a variety of complex samples, including TMT™-labeled peptidesIn order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity and provides excellent orthogonality to low pH reversed-phase LC-MS gradients.The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a highpH fractionation protocol. In contrast to strong cation exchange (SCX) fractionation, the high-pH reversed-phase fractions do not require an additional desalting step before LC/MS analysis.The High pH Reversed-phase Peptide Fractionation Kit includes a high pH buffer (0.1% triethylamine) and twelve spin columns containing pH-resistant reversed-phase resin. Each reversed-phase fractionation spin column enables fractionation of 10–100 µg of peptide sample using a microcentrifuge.Native, phosphorylated, Tandem Mass TagTM (TMTTM)-labeled, and other complex peptide mixture samples can be fractionated using this kit. Combining the search results generated by the individual fractions helps improve protein sequence coverage and increases the number of identified proteins relative to unfractionated samples.Applications:Reducing sample complexity to identify targets of interestConducting systems biology studiesReducing sample complexity to improve quantitation studies

Supplier: MP Biomedicals

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature

Supplier: Cytiva

Streptavidin Sepharose™ High Performance is an affinity chromatography resin designed for fast and reliable, high resolution purification of biotinylated biomolecules.

Supplier: Cytiva

Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC)

Supplier: Cytiva

Capto™ S ImpAct is a strong cation exchange chromatography medium (resin) developed for polishing of monoclonal antibodies (MAbs) and other biomolecules.

Supplier: Cytiva

This media is suitable for affinity chromatography.

Supplier: Cytiva

Capto Butyl is a hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification, Their combination of high capacity, high flow rate and low backpressure reduces process cycle times.

Supplier: Cytiva

PlasmidSelect Xtra is a thiophilic aromatic adsorption chromatography medium with a selectivity that allows generic purification of supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.

Supplier: Cytiva

Phenyl Sepharose High Performance is an aromatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.

Supplier: G-Biosciences

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals

Supplier: Cytiva

Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.

Supplier: G-Biosciences

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.

Supplier: Cytiva

SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high flow velocities and low back-pressure.