4750 Results for: "PHC+Corporation"
P450-Glo CYP3A4 Assay (Luciferin-PPXE) DMSO-Tolerant Assay, Promega
Supplier: Promega Corporation
The P450-Glo CYP3A4 Assay System provides a homogeneous, luminescent method for measuring CYP3A4 activity and the screening system contains a complete set of reagents for performing the assay.
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ADP-Glo Kinase Assay + LYN B Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human LYN B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. LYN is a 56kDa tyrosine kinase that is similar to mouse T-lymphocyte-specific tyrosine kinase p56lck, v-yes, v-fgr and v-src.
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pCI Mammalian Expression Vector, 20 µg, Promega
Supplier: Promega Corporation
The pCI Vector contains the CMV immediate-early enhancer/promoter region and is designed to promote constitutive expression of cloned DNA inserts in mammalian cells.
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pSV-beta-Galactosidase Control Vector, 20 µg, Promega
Supplier: Promega Corporation
A positive control vector for monitoring transfection efficiencies of mammalian cells. The SV40 early promoter and enhancer drive transcription of the lacZ gene, which encodes the beta-galactosidase enzyme.
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PhiX174 DNA/HinfI Markers, 50 µg, Promega
Supplier: Promega Corporation
This conventional DNA digest marker is generated by ΦX174 DNA digested to completion with HinfI to create 20 ethanol-precipitated DNA fragments ranging in size from 24bp to 726bp.
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ADP-Glo Kinase Assay + SRC Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human SRC was expressed in E. coli cells using an N-terminal GST tag. SRC was originally identified as a transforming protein of the Rous sarcoma virus that had enzymatic ability to phosphorylate tyrosine in protein substrates.
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Kinase-Glo Max Luminescent Kinase Assay, Promega
Supplier: Promega Corporation
The Kinase-Glo Luminescent Kinase Assays are homogeneous non-radioactive systems for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction.
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Kinase Selectivity Profiling System: TKL-1 + ADP-Glo Assay, 8 × 50 reactions, Promega
Supplier: Promega Corporation
Easy-to-use kits for performing kinase selectivity profiling that rely on the ADP-Glo Kinase Assay technology. Each system includes kinase and substrate pairs in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.
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ADP-Glo Kinase Assay + ROCK2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
ROCK2 is a ubiquitously expressed serine/threonine kinase localized in the nucleus that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element.
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ADP-Glo Kinase Assay + ALK2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
ALK2 is a receptor serine/threonine kinase that is member of the ALK family and is upstream of signaling pathway involving the SMAD proteins especially SMAD1/5/8.
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ADP-Glo Kinase Assay + ROCK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human ROCK1 (amino acids 17-535) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ROCK1 is a ubiquitously expressed serine-threonine kinase that is a downstream target of the small GTPase RhoA.
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ADP-Glo Kinase Assay with PIP2:3PS, 1,000 assays, Promega
Supplier: Promega Corporation
The ADP-Glo Kinase assay and lipid kinase substrates PIP2:3PS and PI:3PS are components of the PI3K-Glo Class I Kinase Profiling System that are available for purchase separately.
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GoTaq® G2 Hot Start Green Master Mix, Promega
Supplier: Promega Corporation
GoTaq G2 Hot Start Master Mixes are ready-to-use mixes containing GoTaq G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium
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TAK1-TAB1 Kinase Enzyme System, Promega
Supplier: Promega Corporation
Recombinant human proteins TAK1 (1-303) and TAB1 (437-end), linked by a small peptide (DFGGGGG), were expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
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Proteinase K
Supplier: Promega Corporation
Proteinase K, produced by the fungus Tritirachium album Limber, is a serine protease that exhibits broad cleavage activity. Proteinase K (PK) Solution is supplied at a concentration of 20mg/ml in 10mM Tris-HCl (pH 7.5), 1mM calcium chloride, 50% glycerol.
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MAPKAPK5 Kinase Enzyme System, Promega
Supplier: Promega Corporation
Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.
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Kinase Selectivity Profiling System: General Panel, 24 × 50 reactions, Promega
Supplier: Promega Corporation
Easy-to-use kits for performing kinase selectivity profiling that rely on the ADP-Glo Kinase Assay technology. Each system includes kinase and substrate pairs in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.
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ECL Western Blotting Substrate, 250 ml, Promega
Supplier: Promega Corporation
The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots.
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ADP-Glo Kinase Assay + PKC delta Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKC delta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C delta is a member of the protein kinase C (PKC) family of serine-threonine kinases.
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PKA Kinase Enzyme System, 2,500 units, Promega
Supplier: Promega Corporation
Recombinant bovine cAMP-Dependent Protein Kinase (PKA), Catalytic Subunit, is expressed and purified from E. coli. PKA does not require cAMP for activity, and the gene sequence can be found at accession number NM_174584.
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CHK2 Kinase Enzyme System, Promega
Supplier: Promega Corporation
Recombinant full-length human CHK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage.
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ADP-Glo Kinase Assay + ALK4 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human ALK4 (150-end) was expressed in Sf9 cells using an N-terminal GST tag. ALK4 is a member of the subfamily of receptor ser/thr kinases that mediates signaling by the Activins.
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ADP-Glo Kinase Assay + RIPK2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
RIPK2 (RIP2; RICK) is a death domain-containing protein kinase encoding a predicted 540-amino acid protein that contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain.
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JM109 Competent Cells, >10(8) cfu/μg, 1 ml, Promega
Supplier: Promega Corporation
JM109 is a K strain that is recA- and endA- to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening.
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BL21(DE3)pLysS Competent Cells, >10(6)cfu/ug, 1 ml, Promega
Supplier: Promega Corporation
BL21(DE3)pLysS Competent Cells allow high-efficiency protein expression of any gene that is under the control of a T7 promoter and has a ribosome binding site.
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BTK Kinase Enzyme System, Promega
Supplier: Promega Corporation
Recombinant full-length human BTK was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. BTK (also known as Bruton tyrosine kinase) plays a crucial role in B-lymphocyte differentiation and activation.
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GoTaq® G2 Hot Start Colorless Master Mix, Promega
Supplier: Promega Corporation
GoTaq G2 Hot Start Master Mixes are ready-to-use mixes containing GoTaq G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium
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LigaFast Rapid DNA Ligation System, Promega
Supplier: Promega Corporation
T4 DNA Ligase and buffer combination for rapid (5-minute) ligations.
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GoTaq Hot Start Polymerase, Promega
Supplier: Promega Corporation
GoTaq Hot Start Polymerase contains the high-performance GoTaq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 94-95°C for two minutes.
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ADP-Glo Kinase Assay + FLT1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human FLT1 (amino acids 784-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FLT1, also known as fms-like tyrosine kinase 1, is a member of the VEGFR family and is related to the oncogene ROS.