3464 Results for: "PCR"
Cell Systems Human Liver Sinusoidal Endothelial Cells, Primary Cell and Media Company
Supplier: Cell Systems
Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
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Cell Systems Human Lung Microvascular Endothelial Cells, Primary Cell and Media Company
Supplier: Cell Systems
Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
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Cell Systems Human Synovial Microvascular Endothelial Cells, Primary Cell and Media Company
Supplier: Cell Systems
Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
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Cell Systems Human Pancreatic Microvascular Endothelial Cells, Primary Cell and Media Company
Supplier: Cell Systems
Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
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Cell Systems Human Large Intestine Microvascular Endothelial Cells, Primary Cell and Media Company
Supplier: Cell Systems
Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
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Eppendorf® 5920 R Refrigerated Large Centrifuges
Supplier: Eppendorf
The refrigerated Eppendorf Centrifuge 5920 R offers high-throughput performance and advanced temperature management in a very compact design. With a capacity of up to 4×1000 ml/52×50 ml conical tubes, it can spin both plates and tubes in the same bucket for real flexibility.
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Cell Systems Human Coronary Artery Endothelial Cells, Primary Cell and Media Company
Supplier: Cell Systems
Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
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Cell Systems Human Aortic Smooth Muscle Cells, Primary Cell and Media Company
Supplier: Cell Systems
Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
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Eppendorf® 5910 Ri Refrigerated Centrifuges
Supplier: Eppendorf
The refrigerated Eppendorf Centrifuge 5910 Ri stands out with an intuitive touch screen interface and a range of innovative solutions for usability, documentation and user management. Its wide variety of fixed-angle and swing-bucket rotors accommodate a comprehensive spectrum of applications in a compact footprint.
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Cell Systems Human Venous Endothelial Cells (HUVEC), Primary Cell and Media Company
Supplier: Cell Systems
Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
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Cell Systems Human Adult Dermal Microvascular Endothelial Cells, Primary Cell and Media Company
Supplier: Cell Systems
Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
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Combitips® advanced Positive Displacement Pipette Tips, Forensic DNA Grade - Individually Blister-Wrapped
Supplier: Eppendorf
Eppendorf Combitips® advanced in 'Forensic DNA Grade' offer unmatched precision and safety, individually blister-wrapped for maximal DNA contamination prevention. Ideal for forensic applications requiring the utmost accuracy and sterility.
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Antifoam SE-15
Supplier: Aladdin Scientific
Antifoam SE-15 aqueous emulsion for bacterial and mammalian systems.
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epT.I.P.S.® Pipette Tips, BioBased Reloads
Supplier: Eppendorf
Eppendorf epT.I.P.S.® BioBased Reloads in 'Biopur' grade innovate with cutting edge eco-friendly design. The tips are made from 90% plant-based material in a reloadable format. They ensure precision and purity, and are ideal for sensitive applications prioritizing environmental responsibility.
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Cell Systems Human Aortic Endothelial Cells, Primary Cell and Media Company
Supplier: Cell Systems
Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
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Cell Systems Human Arterial Endothelial Cells, Primary Cell and Media Company
Supplier: Cell Systems
Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
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DRG® Chlamydia trachomatis IgG ELISA, DRG International
Supplier: DRG International
An enzyme immunoassay for the qualitative and semiquantitative determination of IgG-class antibodies to Chlamydia trachomatis in serum and plasma.
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Cell Systems Human Coronary Artery Smooth Muscle Cells, Primary Cell and Media Company
Supplier: Cell Systems
Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
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Cell Systems Human Synovial Stromal Cells, Primary Cell and Media Company
Supplier: Cell Systems
Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
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Barnstead™ MicroPure Water Purification Systems, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Barnstead MicroPure water purification systems provides up to 15L/day of Type 1, ultrapure (18.2 megohm) water from pre-treated feed water. All MicroPure systems include:feed water monitoring to protect your consumables, quick discconnects to make changing consumables easy, and all core consumables and tubing. MicroPure-ST systems come standard with a 6 liter integrated feed tank for labs that do not have a ready supply of pre-treated feed water.
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Barnstead™ GenPure™ xCAD Plus Water Purification Systems, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Barnstead GenPure xCAD Plus water purification systems provides up to 200L/day of Type 1, ultrapure (18.2 megohm) water from pre-treated feed water
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Agarose, white powder, high resolution for molecular biology
Supplier: MP Biomedicals
Agarose High Resolution is an intermediate melting agarose with approximately twice the resolution capability of routine agarose. It is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae. It is ideal for the resolution of small DNA fragments in the range 20 bp to 2000 bp.
Agarose high resolution is an intermediate melting and gelling point agarose which exhibits superior sieving characteristics. It is recommended for analytical gels of DNA fragments under 1000 bp and is ideal for small DNA fragments down to 20 bp can be separated on 6 % gels. It resolves fragments differing in size by only 2 bp. Its use avoids the need for PAGE Southern and Northern transfers can be achieved efficiently from them at concentrations up to 2%. DNA fragments can be purified with high efficiency from this agarose gels up to 4% by using either a purification kit or an electroelution system.
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ep Dualfilter T.I.P.S.® SealMax® Filter Pipette Tips, BioBased Reloads
Supplier: Eppendorf
Eppendorf ep Dualfilter T.I.P.S.® SealMax® BioBased Reloads in 'Biopur®' grade blend eco-innovation with ultimate purity. Made from 90% plant-based material in a reloadable format, these self-sealing, dual-filter tips are ideal for sensitive, contamination-free lab applications.
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NanoPhotometer® N60-MOBILE UV/Visible Spectrophotometer for NanoVolume Applications, Implen
Supplier: IMPLEN U.S.A. INC
Implen has become the leading expert for innovative, high-quality spectroscopy instruments and the NanoPhotometer® is trusted by thousands of researchers worldwide.
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NanoPhotometer® C40-TOUCH UV/Visible Spectrophotometer for Standard Cuvette Applications, Implen
Supplier: IMPLEN U.S.A. INC
Implen has become the leading expert for innovative, high-quality spectroscopy instruments and the NanoPhotometer® is trusted by thousands of researchers worldwide.
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XL1 Blue and XL2 Blue Competent Cells, Agilent Technologies
Supplier: Agilent Technologies
XL1 and XL2-Blue Competent Cells are versatile for routine cloning and allow blue-white color screening for high efficiency cloning of methylated DNA.
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NanoPhotometer® N50-TOUCH UV/Visible Spectrophotometer for NanoVolume Applications, Implen
Supplier: IMPLEN U.S.A. INC
Implen has become the leading expert for innovative, high-quality spectroscopy instruments and the NanoPhotometer® is trusted by thousands of researchers worldwide.
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NanoPhotometer® C40-MOBILE UV/Visible Spectrophotometer for Standard Cuvette Applications, Implen
Supplier: IMPLEN U.S.A. INC
Implen has become the leading expert for innovative, high-quality spectroscopy instruments and the NanoPhotometer® is trusted by thousands of researchers worldwide.
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ClonePix™ 2 Mammalian Colony Picker, Molecular Devices
Supplier: Molecular Devices
Redefine clone screening and selection: transform your cell line development workflow.
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Urea ≥98%, white powder for molecular biology
Supplier: MP Biomedicals
Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C