Supplier: Promega Corporation

REAGENTS ORGANIC KIT 96 WATER-GLO

Supplier: MP Biomedicals

Uridine is a nucleoside, contains an uracil attached to a ribose ring (known as a ribofuranose) via a β-N1-glycosidic bond. It is an organic compound of the pyrimidine family, that occurs as one of the four bases coding genetic information in the polynucleotide chain of RNA.
Uridine can increase the rate-limiting step of Phosphatidylcholine synthesis in the brain, Phosphatidylcholine is the major structural and functional component of brain cell membranes. Without phosphatidylcholine, brain cells lose their youthful function and suffer the degenerative structural changes associated with aging. Uridine is thought to aid Alzheimer's Disease due to helping with synaptic connections, which appear to be reduced in the state of Alzheimer's.
Physical Appearance: White Powder
UV/Visible Absorbance: λ max (water)= 260 - 263 nm
Solubility: Soluble in 0.1 N HCl (50 mg/mL-clear, colorless solution) and water (50 mg/mL)

Supplier: MP Biomedicals

Arachidonic Acid is an essential fatty acid. Occurs in liver, brain, glandular organs, and depot fats of animals, in small amounts in human depot fats, and is a constituent of animal phosphatides.
Arachidonic Acid is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes. Arachidonic acid plays a key role in cellular regulation and is controlled through multiple interconnected pathways.
Arachidonic acid (AA) is an unsaturated ω6 fatty acid constituent of the phospholipids of cell membranes. Phospholipase A2 releases AA from the membrane phospholipids in response to inflammation. AA is subsequently metabolized to prostaglandins and thromboxanes by at least two cyclooxygenase (COX) isoforms, to leukotrienes and lipoxins by lipoxygenases, and to epoxyeicosatrienoic acids via cytochrome p450-catalyzed metabolism. AA and its metabolites play important roles in a variety of biological processes, including signal transduction, smooth muscle contraction, chemotaxis, cell proliferation and differentiation, and apoptosis. AA has been demonstrated to bind to the a subunit of G protein and inhibit the activity of Ras GTPase-activating proteins (GAPs). Cellular uptake of AA is energy dependent and involves protein-facilitated transport across the plasma membrane.
If ethanol is undesirable, arachidonic acid may be dissolved in acetonitrile, DMF, or DMSO. Simply evaporate the ethanol under a gentle stream of nitrogen (be certain not to evaporate the material to dryness) and redissolve the arachidonic acid in the solvent of choice.Just prior to use, make dilutions of the stock solution into aqueous buffer or isotonic saline to bring the arachidonic acid to the desired concentration. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiologic effects at low concentrations. A control using the solvent in the absence of the prostaglandin will address this potential variable. We do not recommend storing the aqueous solution for more than one day. It is difficult to obtain aqueous solutions of arachidonic acid directly. However, an organic solvent free solution of arachidonic acid can be prepared using concentrated basic buffers (pH > 8.0 and ionic strength not less than 0.1 M). Add 400 μL of cold buffer (0 °C) per mg of arachidonic acid and agitate vigorously and/or ultrasonicate.

Supplier: G-Biosciences

Lysis and extraction of biologically active proteins from cellular and tissue samples is the first critical step for biochemical analysis

Supplier: Rigaku Reagents

Discover more protein crystallization hits with this unique screen by employing synergistic effects of mechanistically distinct precipitating agents (salts, organic solvents, polyethylene glycols, additives, pH).

Supplier: MP Biomedicals

Chrome Azurol S is used as a reagent for determination of fluoride.

Supplier: Epredia

Improve throughput with the popular Gemini™ AS Automated Slide Stainer, the ultimate multitasking stainer with a footprint small enough to place exactly where it is needed for optimal laboratory workflow.

Supplier: MP Biomedicals

HEPES is a zwitterionic organic chemical buffering agent.

Supplier: New England Biolabs (NEB)

Cell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. Traditionally, RNA is extracted and purified from treated cells via column-based or chemical methods. The Luna Cell Ready Lysis Module is a fast, convenient, and sensitive alternative for evaluating RNA expression levels without performing traditional RNA extraction steps.

Supplier: MP Biomedicals

HEPES is a zwitterionic organic chemical buffering agent.
HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.

Supplier: MP Biomedicals

Choline chloride is an organic compound and a quaternary ammonium salt. It is a weak acid. Choline chloride is the salt of the naturally occurring choline, the pre-stage of the neurotransmitter acetylcholine, which is important for mnemonic and thought-processes. Choline occurs naturally in fungi, hop and kingcups and as integral part of lecithin. Choline chloride is a common food additive in animal husbandry
The enzymatic activities of butyrylcholinesterase (BChE) and paraoxonase 1 (PON1), two serum enzymes synthesized by the liver and related with inflammation, were decreased in a sepsis animal model injected with LPS. Choline chloride administered intravenously at 20 mg/kg body weight prevents the LPS-mediated decreases in the activities of these two enzymes.
Choline chloride is used as a source of methyl groups for methyl-transfer reactions.
Physical Appearance: Hard, White Crystalline Powder
pH: Neutral to Litmus
Vapor Pressure: 10 hPa at 20 °C (Lit.)
Partition Coefficient: n octanol/water (logKow): -3.77 at 25 °C (lit.)
Solubility: Soluble in water: (10% aq soln) Clear, Colorless Solution

Supplier: MP Biomedicals

Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.

Supplier: Honeywell Research Chemicals

ACS GENERAL USE SOLVENTS

Supplier: MP Biomedicals

Cycloheximide is a glutarimide antibiotic derived from a microbial source. Cycloheximide is an antibiotic which is very active against many molds, yeasts, and phytopathogenic fungi. It exhibits somewhat lower activity against bacteria and certain fungi. Control of various molds and fungi in gelatin-based photographic emulsions, photoengraving glues, and other light-sensitive products is suggested.
Cycloheximide is used in plant research to study disease resistance and as an ethylene stimulant, useful in studies involving fruit and leaf production. It is also used in bacteriological media to isolate or count bacteria in the presence of yeast and molds; Used in protein synthesis in apoptosis; Gene expression; Glycogenolysis, gluconeogenesis and ureogenesis in isolated rat hepatocytes; Studies involving steroidogenesis; Used in plant regulation and as a quality control measure by the food and beverage industry.
Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors. Cycloheximide inhibits peptide synthesis in eukaryotic organisms but not in prokaryotes. Protein synthesis is blocked by the interaction of cycloheximide with the translocase enzyme. This interaction prohibits the translocation of messenger RNA on the cytosolic, 80S ribosomes without inhibiting organelle protein synthesis. Cycloheximide is also known to induce FAS/FAS Ligand apoptosis, and triggers apoptosis in HL-60 cells, T-cell hybridomas, Burkitt's lymphoma cells in addition to a variety of other cell types. Cycloheximide will also delay or inhibit apoptosis induced by other agents.

Supplier: Honeywell Research Chemicals

ACS GENERAL USE SOLVENTS

Supplier: Ricca Chemical

APHA and ASTM for organic nitrogen.

Supplier: Honeywell Research Chemicals

ACS GENERAL USE SOLVENTS

Supplier: Honeywell Research Chemicals

ACS GENERAL USE SOLVENTS

Supplier: Azer Scientific Inc.

Pathology containers are designed for collection, transport, and storage of powders, liquids, solids, and biological specimens.

Supplier: Honeywell Research Chemicals

ACS GENERAL USE SOLVENTS

Supplier: Honeywell Research Chemicals

ACS GENERAL USE SOLVENTS

Supplier: Ricca Chemical

50% - 2.5% (w/v) Aqueous. APHA, ASTM and EPA for Organic Nitrogen. Container: Cubitainer.

Supplier: TrippNT

White PVC storage bin with 8 adjustable compartments and 2 shelves creates storage to fit supply volume.

Supplier: Ricca Chemical

Pomeroy, ASTM D 888, for Dissolved Oxygen (DO). High DO (15 mg/L - 40 mg/L) and samples with high Organic concentration. Container: Plastic.

Supplier: Agilent Technologies

The RecoverEase DNA isolation kit recovers high-molecular-weight (100 to 500 kb) genomic DNA that is fully hydrated and ready for multiple applications the next day. DNA can be isolated from whole tissues such as liver, spleen, kidney, brain and testes without hazardous and tedious organic extractions or ethanol precipitations. The RecoverEase DNA isolation kit quickly isolates high-molecular-weight genomic DNA from a variety of tissues without organic solvent extractions or ethanol precipitation;

Supplier: Promega Corporation

The Wizard® Plus Midipreps DNA Purification System isolates plasmid DNA in 90 minutes or less, yielding up to 200μg of high-quality DNA with no organic extractions or ethanol precipitations.

Supplier: New England Biolabs (NEB)

The New England Biolabs Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents.

Supplier: Ricca Chemical

Methylene blue, Loeffler formulation

Supplier: Promega Corporation

A simple, reliable, silica-resin-based method for isolation of plasmid DNA on a maxiprep scale. No organic extraction or ethanol precipitation required. Yields 300ug-1mg from 100-500ml culture.

Supplier: Spectrum Chemical Mfg. Corp.

Acetic Acid, Glacial, FCC is used in the pickling of vegetables and other foods. Spectrum Chemical offers over 300 Food Grade (FCC) chemical ingredients packaged in laboratory size bottles to production drum quantities and are manufactured, packaged and stored under current Good Manufacturing Practices (cGMP) per 21CFR part 211 in FDA registered and inspected facilities.