1772 Results for: "Labeling and Detection"

Supplier: Adipogen

Amino derivative of biotin that can be used as an intracellular label for cells, particularly neurons. It is used for neuronal tracing studies by visualizing neural architecture and for the identification of gap junction coupling. It is better soluble and non-toxic compared to other neuronal labels. It remains longer in cellsand can be fixed with formalin or glutaraldehyde. It can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods.

Supplier: Anaspec

This gelatin is heavily labeled with FITC, and the conjugate is a highly quenched gelatinase/collagenase substrate. It is efficiently digested by gelatinases and collagenases, releasing brightly fluorescent peptides. The increase in fluorescence upon digestion is proportional to proteolytic activity. Longer incubation may increase its sensitivity for detecting proteases.

Supplier: G-Biosciences

A novel agent for fast and sensitive detection of DNA synthesis in vivo1. 5-EdU is incorporated into proliferating cells followed by detection with a fluorescent azide using click ligation1. In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. May be used for studying cell proliferation in the central nervous system and may be combined with BrdU staining in a novel protocol allowing for double labeling of DNA synthesis.

Supplier: Abcam

Fluorescently-labeled deoxyglucose analog, used to directly monitor glucose uptake by living cells and tissues. Also used as a topical contrast reagent for the detection of neoplasia. Uptake can be monitored by real-time confocal, high-resolution or wide-field fluorescence microscopy and flow cytometry.

Supplier: Cytiva

Amersham CyDye NIR-labeled secondary antibodies enable quantitation of Western blots with a strong signal-to-noise ratio. Mulitplexed experiments allow for the detection of two proteins on a single blot. The use of total lane signals to normalize target protein data yield robust and reliable results.

Supplier: Genetex

This Myc tag antibody, clone 9E10, is excellent for immunoprecipitation and Western blotting of fusion proteins labeled with EQKLISEEDL tag. 9E10 recognises recombinant proteins incorporating a c-Myc epitope tag. 9E10 also detects human c-Myc protein and a peptide in random coil configuration not as a helix.

Supplier: Cytiva

Amersham CyDye NIR-labeled secondary antibodies enable quantitation of Western blots with a strong signal-to-noise ratio. Mulitplexed experiments allow for the detection of two proteins on a single blot. The use of total lane signals to normalize target protein data yield robust and reliable results.

Supplier: Cytiva

Amersham CyDye NIR-labeled secondary antibodies enable quantitation of Western blots with a strong signal-to-noise ratio. Mulitplexed experiments allow for the detection of two proteins on a single blot. The use of total lane signals to normalize target protein data yield robust and reliable results.

Supplier: Cytiva

Amersham CyDye NIR-labeled secondary antibodies enable quantitation of Western blots with a strong signal-to-noise ratio. Mulitplexed experiments allow for the detection of two proteins on a single blot. The use of total lane signals to normalize target protein data yield robust and reliable results.

Supplier: Anaspec

AnaSpec offers two types of fluoresceinated collagen (type I). This product is water soluble and is used for quick fluorometric measurement of collagenase activity. In this protease substrate, collagen is heavily labeled with FITC, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. This fluoresceinated collagen is useful for detecting MMP-1 activity.

Supplier: G-Biosciences

Reagent for use in catalyzed reporter deposition (CARD) signal amplification protocols in a variety of immunoassays in which, horseradish peroxidase- catalyzed deposition of biotinyl tyramide is detected with labeled streptavidin1. Widely used for signal amplification in fluorescent in situ hybridization (FISH) protocols2. May be used for signal amplification in a sandwich ELISA for quantification of α-tubulin isotypes3. Reagent for “self-biotinylation” of DNA G-quadruplexes4. May be used with APEX-mediated biotin labeling to identify protein-protein interactions.

Supplier: Abcam

Anti-NeuN antibody EPR12763 is a rabbit monoclonal Alexa Fluor® 647 conjugated antibody that is used to detect NeuN in IHC and ICC/IF. Suitable for Human, Mouse and Rat samples. - Cited in over 30 publications - NeuN neuronal marker - Directly labelled to save you time and simplify your experiment.

Supplier: Thermo Scientific

The Thermo Scientific™ GlycanPac™ AXR-1 column is a high-performance, silica-based HPLC column for the simultaneous separation of glycans by charge, isomerism, and size. It is designed to provide industry-leading resolution with unique selectivities for biologically important glycans, either labeled or native, using either fluorescence and/or mass spectrometry (MS) detection.

Supplier: Adipogen

Florescent probe that is commonly used as a building block to synthesize coumarin-type fluorescent probes. Spectral data: lambdaex 320 nm, lambdaem 380 nm in methanol. It is used as a fluorescent label for peptides. It is widely used for developing FRET peptide substrates for analyzing protease activities and for HPLC derivatization by fluorescence detection.

Supplier: Adipogen

3-(2-Furoyl)quinoline-2-carboxaldehyde (FQCA) is a fluorogenic derivatizing reagent for liquid chromatography suitable for high-sensitivity analysis of amino acids by laser-induced fluorescence with an argon-ion laser. The reagent forms highly fluorescent isoindoles upon reaction with primary amines. It is used for the determination of isoelectronic points (pI values) of proteins with laser-induced fluorescence detection (c-IEF-LIF). C-IEF-LIF technique can routinely detect 10-11 M of FQCA-labeled protein, compared with 10-7 M by traditional slab gel IEF and silver staining.

Supplier: Enzo Life Sciences

Fluorescein-12-UTP is ideal for in vitro transcription reactions catalyzed by T3, T7, or SP6 RNA polymerases and reverse transcriptase to produce fluorophore-labeled RNA transcripts. RNA-based probes can be used to identify complementary sequences by in situ hybridization to fixed cells and tissues by direct or indirect fluorescence detection or by membrane hybridization procedures.

Supplier: Anaspec

The SensoLyte® Green Protease Assay Kit is widely used for detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity (Ex/Em=488/520 nm). The SensoLyte® protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases.

Supplier: Invitrogen

HRP-Conjugated Streptavidin consists of streptavidin protein that is covalently conjugated to horseradish peroxidase (HRP) enzyme (RZ > 3.0). Streptavidin binds to biotin and the conjugated HRP provides enzyme activity for detection using an appropriate substrate system. This particular product has been used primarily in sandwich ELISA applications to provide consistent measurement of biotinylated detection antibodies.

Formulation: Provided in a conjugate stabilizer containing 0.1% ProClin Compound as a preservative.
Shipped on cold packs.
Application: ELISA: to be used with biotin-labeled detection antibodies. Recommended dilution range is from 1:4,000 to 1:20,000. The optimal dilution must be determined empirically for each specific application. Prepare dilutions immediately before use.

Supplier: ARBOR ASSAYS MS

Arbor Assays' Cystatin C ELISA Kit provides accurate quantification of human Cystatin C in serum, plasma, urine, and tissue culture media, using a high-sensitivity sandwich ELISA format. With a sensitivity of 0.058 ng/ml and a detection range of 0.156 to 10 ng/ml, this assay is ideal for evaluating kidney function and related diseases. The 2 hour assay utilizes a mouse monoclonal capture antibody and a peroxidase-labeled detection antibody to deliver reliable results across a range of research applications including inflammation, cardiovascular health, neurodegenerative disorders, and renal pathology.

Supplier: Bioss

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

Supplier: Bioss

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

Supplier: Bioss

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

Supplier: Bioss

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

Supplier: Bioss

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

Supplier: Antibodies.com

Porcine IgG Matched Antibody Pair kit includes an unlabeled monoclonal capture antibody, an HRP-labelled monoclonal detection antibody, Porcine IgG ELISA standard, and standard reconstitution buffer. This matched antibody pair kit can be used to quantify native and recombinant porcine IgG. Matched antibody pair kits are ideal for economical ELISA and ELISA-based assay development.

Supplier: Bioss

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

Supplier: Alkali Scientific

In addition to its neutralizing capabilities, the CellPro™ Neutralizing Solution is compatible with a wide range of laboratory techniques, including Southern and Northern blotting, where it plays a crucial role in preparing samples for hybridization to labeled probes. The solution's effectiveness in neutralizing harsh chemical conditions makes it an indispensable component in molecular biology workflows, supporting the accurate detection and analysis of nucleic acids.

Supplier: Bioss

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

Supplier: QUALITY BIOLOGICAL, INC.

Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk to reduce the background. Nonfat dried milk is often preferred as it is inexpensive and widely available. However, milk proteins are not compatible with all detection labels, so care must be taken to choose the appropriate blocking solution.

Supplier: Antibodies.com

Mouse IgG Matched Antibody Pair Kit includes an unlabeled polyclonal capture antibody, an AP-labelled polyclonal detection antibody, native mouse IgG ELISA standard, and standard reconstitution buffer. This matched antibody pair kit can be used to quantify native and recombinant mouse IgG. Matched antibody pair kits are ideal for economical ELISA and ELISA-based assay development.