1770 Results for: "Labeling and Detection"

Supplier: MP Biomedicals

Dansyl Chloride is a fluorogenic reagent for fluorescent N-terminal derivatization of amino acids and peptides and detection by reverse phase HPLC. It is used in labeling proteins and amino acids.

Supplier: Cytiva

Hybond™ ECL™ gives excellent sensitivity, resolution, and low background for all labelling and detection systems (radioactive, non radioactive, and chromogenic), especially when used with ECL™, ECL™ Prime, and ECL™ Plex Western Blotting Detection kits and reagents. Special packaging features include interleaf on both sides of the membrane and air-tight resealable aluminium bag with label space for marking the 'opened on' date.

Supplier: Enzo Life Sciences

The PathoGene® HPV Type 16/18 Probe is a mixture of biotin-labeled HPV 16 and HPV 18-specific probes in buffered formamide and hybridization enhancers.

Supplier: Biotium

Live-or-Dye NucFix™ Red is a red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected by flow cytometry or fluorescence microscopy.

Supplier: Bioss

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast,insect and mammalian cell systems.

Supplier: Enzo Life Sciences

Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labeled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.

Supplier: Biotium

CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

Supplier: Rockland Immunochemical

Epitope tag antibody sampler kit detects overexpressed fusion proteins containing the corresponding epitope tags. DYKDDDDK Tag Antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies). Epitope tags are useful for the labeling and detection of proteins using western blotting, immunoprecipitation, and immunostaining techniques.

Supplier: VWR

A small biomolecule, 244 Daltons, that has high binding affinity to avidin and streptavidin often used to label antibodies and enzymes. Several molecules of biotin can react with a single protein molecule to increase the sensitivity at the site of detection.

Supplier: Invitrogen

A complete system for detection of biotin-labeled nucleic acids for various blotting applications including Northern/Southern blots, ribonuclease protection assays (RPAs), electrophoretic mobility shift assays (EMSAs or gel shifts).

Supplier: MP Biomedicals

Phenol is useful for extraction of viral, plasmid and phage DNAs, and to solubilize and dissociate proteins. It is used as a general disinfectant, either in solution or mixed with slaked lime, etc., for toilets, stables, cesspools, floors, drains, etc.; for the manufacture of colorless or light-colored artificial resins, many medical and industrial organic compounds and dyes; as a reagent in chemical analysis. Aqueous solutions are used as topical anesthetic; topical antiseptic; topical antipruritic. Phenol is used in DNA extractions for removing protein.

Supplier: Prosci

This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this mAb is also suitable for the conversion of fluorescein-labeled detection into immuno-enzymatic assays.

Supplier: Adipogen

The reagent, a cyanine dye, can be used as a label for the determination of proteins with NIR-fluorescent technology. The absorption-maxima of the reagent lies in a region of low interference by other biomolecules. The isothiocyanate-moiety is used to link the reagent to proteins which can sensitively be detected. Experiments to lower the detection-limits of immunoassays have been done.

Supplier: Invitrogen

Thermo Scientific Pierce EZ-Link Phosphine-PEG3-Biotin is a biotinylation reagent for labeling azide-containing molecules, which enables biotin-based detection and affinity purification of molecules via Staudinger ligation strategies.

Supplier: Enzo Life Sciences

Red 580 [5-ROX] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 580 dUTP can also be used for multicolor fluorescence labelling.

Supplier: Abcam

Water-soluble, long-chained biotin hydrazide for glycoconjugate detection. Selective label for sialic acid, galactose and other sugars in glycoconjugates using avidin-biotin technology. Can biotin label proteins and peptides. Linking arm enables easier binding to avidin/sterptavidin in ELISA. Cellular tracer. Can be introduced into cells via micro-injection.

Supplier: Abcam

HRP/DAB detection IHC kit, mouse & rabbit specific. Is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody. - Cited in over 220 publications - HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB

Supplier: TRIBIOSCIENCE, INC. MS

Caspase 3 colorimetric assay provides a simple and convenient approach for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p nitroaniline pNA after cleavage from the labeled substrate DEVD pNA.

Supplier: Enzo Life Sciences

Cyanine 3-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 3-dUTP can also be used for multicolor fluorescence labeling.

Supplier: Enzo Life Sciences

Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.

Supplier: TRIBIOSCIENCE, INC. MS

The Caspase-1 Activity Fluorometric Assay Kit provides a simple and convenient method for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on the detection of the free AFC fluorescence after cleavage from the labeled substrate YVAD-AFC.

Supplier: Thermo Scientific Chemicals

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes; particularly useful in ELISA techniques, blue result can be read spectrophotometrically at 370 or 650 nm3,3',5,5'-Tetramethylbenzidine is used as a clinical reagent for testing blood.

Supplier: Enzo Life Sciences

Orange 552 dUTP (5-TAMRA-dUTP) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Orange 552 dUTP can also be used for multicolor fluorescence labeling.

Supplier: TRIBIOSCIENCE, INC. MS

The Caspase-1 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p- nitroaniline (pNA) after cleavage from the labeled substrate YVAD-pNA.

Supplier: Genetex

Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromato-graphic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induction and repair of these lesions without the requirement of in vivo radiolabeling of DNA. Photoimmunodetection, is a rapid, reliable and low-cost supplement to existing methods for nonradioactive DNA labeling. It enables a sensitive and non-radioactive method for labeling, detection, and quantification of high molecular weight (HMW) DNA fragments. The method is based on the introduction of thymine dimers into DNA after separa-tion by pulse field gel electrophoresis (PFGE), followed by detection with thymine dimer specific antibodies. The method does not require any enzymatic or chemical manipulation of the DNA sample. Monoclonal anti-bodies reacting specifically with thymine dimer, facilitate investigations on the apoptotic process and the role of UV-induced pyrimidine dimers in the process of photocarcinogenesis.

Supplier: Biotium

CF® Dye alkynes react with azide groups via copper-catalyzed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® Dye alkynes can also be used as building blocks to form fluorescent polymers.

Supplier: Biotium

CF®DI dye hydrazides react with molecules containing aldehyde or ketone groups to form a hydrazone bond. The dyes are size- and charge-matched and do not significantly alter the molecular weight or pI of the labeled proteins. They are used for detection of protein carbonylation by fluorescence 2D-DIGE.

Supplier: Adipogen

Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents. Oligonucleotide conjugates of FITC are frequently employed as hybridization probes. Peptide conjugates of FITC and other fluorescent isothiocyanates are susceptible to Edman degradation, making them useful for high-sensitivity amino acid sequencing, FITC-labeled amino acidd and peptides have been separated by capillary electrophoresis with a detection limit of fewer than 1000 molecules. FITC has also been used to detect proteins in gels and on nitrocellulose membranes and is a selective inhibitor of several membrane ATPases.

Supplier: Adipogen

Amino derivative of biotin that can be used as an intracellular label for cells, particularly neurons. It is used for neuronal tracing studies by visualizing neural architecture and for the identification of gap junction coupling. It is better soluble and non-toxic compared to other neuronal labels. It remains longer in cellsand can be fixed with formalin or glutaraldehyde. It can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods.

Supplier: Abcam

Amino derivative of biotin that can be used as an intracellular label for cells, particularly neurons. Used for neuronal tracing studies by visualizing neural architecture and for the identification of gap junction coupling. More soluble and non-toxic compared to other neuronal labels. Remains longer in cells and can be fixed with formalin or glutaraldehyde. Can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods.