1762 Results for: "Labeling and Detection"

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

ECL Direct™ nucleic acid labelling and detection systems are based on the direct labelling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalysed breakdown of luminol. Suitable for fast and easy detection of medium to high target amounts in applications such as colony/plaque screens, dot blots and PCR product analyses.

Supplier: AAT Bioquest

Biotin NTA (BNTA), a biotin-labeled nitrilotriacetic acid, is widely used to detect histidine-tagged proteins immobilized on nitrocellulose membranes with the detection limite of ~0.1 pmol.

Supplier: Cytiva

AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).

Supplier: Brady Worldwide

ToughWash® material polyester film with acrylic adhesive is a strong, print-on-demand label specially formulated for the Food and Beverage processing industry.

Supplier: Molecular Devices

Screen and select high value mammalian clones with label-free, in situ detection of IgG secretion with the ClonePix system, and other cell analyzers with a green fluorescent channel.

Supplier: AAT Bioquest

This azadibenzocyclooctyne-cyanine dye derivative is a versatile labeling reagent for detection of azide containing molecules or compounds.

Supplier: AAT Bioquest

This azadibenzocyclooctyne-cyanine dye derivative is a versatile labeling reagent for detection of azide containing molecules or compounds.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.

Supplier: Invitrogen

Thermo Scientific DyLight Fluor Phosphine Reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.

Supplier: KPL

LumiGLO Peroxidase Chemiluminescent substrate is an all-purpose, luminol-based substrate designed for the rapid and sensitive detection of peroxidase labeled-conjugates in Western blotting and ELISA

Supplier: KPL

LumiGLO Reserve is a luminol-based chemiluminescent substrate designed for rapid and sensitive detection of peroxidase-labeled conjugates

Supplier: AAT Bioquest

Fluorescence-based methods have many advantages for biological detections in terms of sensitivity.

Supplier: AAT Bioquest

2-NBDG is a fluorescently-labeled deoxyglucose analog that is used primarily to directly monitor glucose uptake by living cells and tissues.

Supplier: AAT Bioquest

Biotin/avidin complexes are widely applied for a variety of biological detections.

Supplier: AAT Bioquest

Biotin/avidin complexes are widely applied for a variety of biological detections.

Supplier: AAT Bioquest

DNA fragmentation represents a characteristic of late stage apoptosis.

Supplier: AAT Bioquest

Biotin/avidin complexes are widely applied for a variety of biological detections.

Supplier: AAT Bioquest

DNA fragmentation represents a characteristic of late stage apoptosis.

Supplier: AAT Bioquest

Biotin/avidin complexes are widely applied for a variety of biological detections.

Supplier: AAT Bioquest

This water-soluble reagent has strong green fluorescence with great Stokes shift.

Supplier: AAT Bioquest

Biotin/avidin complexes are widely applied for a variety of biological detections.

Supplier: KPL

SureBlue Reserve™ provides maximum sensitivity for detection of peroxidase labeled conjugates in a microwell format and provides 50% more sensitivity than standard SureBlue™ TMB substrate

Supplier: Cytiva

Amersham™ CyDye™ fluors combine exceptional photostability with bright and intense signals, resulting in high sensitivity and a highly reproducible performance at a biologically suitable pH range of 3 to 10.

Supplier: Tonbo Biosciences

The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.

Supplier: Adipogen

For the labeling of amino acids and their detection in picomolar amounts.

Supplier: Tonbo Biosciences

The fragmentation of genomic DNA by cellular nucleases during the later stages of apoptosis is also one of the most easily measured features of apoptotic cells. Nuclease activity generates DNA fragments ranging from ~300 bp to 50 bp in length, resulting in a typical DNA ‘laddering’ appearance when analyzed by agarose gel electrophoresis. These fragments have exposed 3’-hydroxyl (OH) ends which can be labeled with bromolated deoxyuridine triphosphates (Br-dUTP). An enzyme, terminal deoxynucleotidyl transferase (TdT), is used to catalyze the template-independent addition of Br-dUTP to the 3’-OH ends of double or single stranded DNA. This method is often called TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) or end labeling. Sites where the Br-dUTP is incorporated can then be detected with an antibody specific to BrdU. With the APO-BrdUTM Kit cells are first labeled with Br-dUTP, and then sites of incorporation are detected through staining with a FITC anti-BrdU antibody. Samples can then be analyzed via flow cytometry. Samples that are apoptotic will stain brightly with the anti-BrdU antibody due to the substantial number of exposed 3’-OH sites, while cells that are non-apoptotic will not have incorporated significant amounts of Br-dUTP and will stain dimly. The APO-BrdU Kit is shipped in one container and consists of two packages. Upon arrival one should be stored at 2-8°C and the other at -20°C.

Supplier: Invitrogen

Thermo Scientific Pierce ONPG is the preferred colorimetric substrate for ELISA applications involving beta-galactosidase (b-Gal) as the reporter enzyme.

Supplier: Thermo Scientific Chemicals

Applications: Fluorescent labeling reagent. used in HPLC for the detection of carboxylic acids