2209 Results for: "Immunology"

Supplier: Rockland Immunochemical

10X Tris buffered saline (TBS) pH 7.5

Supplier: G-Biosciences

Indazole-3-carboxylic acid derivative that inhibits mitochondria-bound hexokinase, reducing aerobic and anaerobic glycolysis in Ehrlich ascites tumor cells (IC50=90 and 45 µM, respectively)1. Causes intracellular acidification by inhibition of lactate transport2. Also inhibits the mitochondrial pyruvate carrier (Ki 2.5 μM) and monocarboxylate transporter isozymes3, as well as the succinate-ubiquinone reductase activity of respiratory complex II4. Enhances infection by oncolytic M1 virus via downregulation of antiviral immune response.

Supplier: G-Biosciences

Agonist of the orphan nuclear receptors RORα and RORγ.1 It attenuated intrapancreatic pathological changes in mouse models of chronic pancreatitis via restoration of the circadian stabilizing loop.2 SR1078 stimulation of RORα restored clock protein BMAL1 expression and oscillation and blocked MYCN-mediated neuroblastoma growth.3 It reduced repetitive behavior is a mouse model of autism suggesting aq potential therapeutic pathway for the disorder.4 SR1078 treatment lead to an increase in p53 levels and increased apoptosis in HepG2 cells.

Supplier: G-Biosciences

RepSox is a potent inhibitor of the TGF-β type I receptor (ALK5), IC50 ALK5 auto-P= 4 nM and IC50 TGF-β cellular assay = 23 nM1. RepSox can replace Sox2 in reprogramming adult cells into pluripotent stem cells via induction of Nanog transcription2,3. It was able to convert astrocytes into neuronal cells as part of a small molecule cocktail (along with valproic acid and CHIR99021)4,5.

Supplier: G-Biosciences

Etomoxir (828934-41-4) is an irreversible inhibitor of mitochondrial carnitine palmitoyl transferase 1 (CPT1).1 It is widely used to study fatty acid oxidation. Etomoxir has been investigated as a therapeutic agent for heart disease2, diabetes3, and cancer4,5. Use of etomoxir in concentrations greater than 5 µM induces acute production of ROS with associated evidence of severe oxidative stress in proliferating T cells indicating a loss of specificity for CTP1 at these concentrations.6 200 µM etomoxir inhibited complex I of the electron transport chain.

Supplier: G-Biosciences

First-in-class selective RNA polymerase I inhibitor, suppressing Pol I transcription in MV 4;11 myelomonocytic leukemia cells and SR immunoblastic lymphoma cells with EC50 values of 95 and 135 nM respectively and demonstrating 200-fold selectivity over Pol II1,2. Activates the DNA damage response3. It induces a rapid accumulation of cytosolic DNA which results in upregulation of STING4. Acts as a potent immunosuppressant inhibiting T cell-mediated alloimmunity and representing a novel class of immunosuppressant.

Supplier: G-Biosciences

CPI-444 (1202402-40-1) is a potent (Ki = 3.54 nM) and selective adenosine A2A receptor antagonist. It blocked the induction of cAMP induced by NECA in HEK-293 cells with an IC50 = 17.03 nM. CPI-444 monotherapy or in combination with anti-PD-1, anti-PD-L1, and anti-CTLA-4 induced T-cell-mediated tumor responses, inhibited tumor growth, and enabled antitumor immune memory.1,2 Currently in clinical trials for multiple cancers.

Supplier: G-Biosciences

Natural product that acts as an inducer of human AhR-dependent gene expression with 10-fold greater potency over the endogenous agonist TCDD1. Indirubin also inhibits CDKs (IC50 = 50–100 nM) and GSK-3β (IC50 = 5-50 nM)2. Ameliorates the effects of a high-fat diet on mice3. Breaks the inflammatory cycle in in vitroand in vivomodels of psoriasis4. Inhibits angiogenesis and causes autophagy and apoptosis in cancer cells.

Supplier: G-Biosciences

A selective, ATP-competitive and reversible inhibitor of mitotic kinesin Kif18A, IC50=1.7 µM1,2. Binds to an allosteric site near Kif18A loop 5 preventing a range of conformational changes thereby blocking motor function3. Inhibits the differentiation of mouse bone marrow cells to dendritic cells4. Suppresses the replication of influenza A virus, inhibiting activation of AKT, p38 MAPK, SAPK and RanBP3 leading to the prevention of nuclear export of viral ribonucleoprotein complexes. Greatly improves the viability of IAV-infected mice.

Supplier: G-Biosciences

Potent (IC50=13 nM) and selective ATP-competitive inhibitor of DNA-dependent protein kinase (DNA-PK).1,2 It increased the toxicity of ionizing radiation and etoposide on SW620 human colon cancer lines via impeding DNA double-strand break repair.3 NU7441 also increased the radio/chemosensitivity of various other cancer cell lines.3-6 Reduces the frequency of NHEJ repair while enhancing the rate of HDR repair following CRISPR/Cas9-mediated gene editing.

Supplier: Rockland Immunochemical

MIP-3α Mouse Recombinant control protein

Supplier: Thermo Scientific Chemicals

This kit consists of highly specific Involucrin antibody (rabbit) and other reagents to peroxidase stain human involucrin in tissue sections.

Supplier: Thermo Scientific Chemicals

A peroxidase substrate for ELISA procedures

Supplier: G-Biosciences

Potent inhibitor of NFκB in T-lymphocytes with almost complete inhibition at 200 nM1. Completely inhibits IL-4 and IFN-γ production and suppresses IL-2 and TNF-α production at 50 nM in T-cells, without inhibiting AP-1 and NF-κB2. Immunosuppresive activity is due to inhibition of cytokine gene expression via blocking NF-AT activity. Has anti-cancer properties via various pathways including ERK inhibition3, ATM/ATR-Chk1/Chk2 activation4, and p38 and JNK activation5.

Supplier: G-Biosciences

GSK583 (1346547-00-9) is a highly selective and potent inhibitor of RIP2 kinase, IC50=5 nM human and IC50=2 nM rat.1 It also potently inhibits isolated RIP3 kinase (IC50=5 nM) but is inactive against RIP3 in cellular assays due to a very low KM,ATP. GSK583 blocks NOD2 signaling by interfering with XIAP-RIP2 binding resulting in decreased cytokine and chemokine production.

Supplier: G-Biosciences

A novel truncated analog of lipoxin A4 which retains anti-inflammatory activity.1 Inhibits LTB4-induced leukocyte chemotaxis, IC50=5 nM).1 Attenuates hemorrhagic shock-induced acute lung injury in a rat model.2 Displays hepatoprotective effects in acetaminophen-induced liver injury in mice.3 Limits inflammatory damage in the cerebral cortex and helps maintain blood brain barrier integrity in a rat model of ischemic stroke.4 Attenuates renal ischemia/reperfusion injury via activation of p38 MAPK/PPARα/HO-1 pathway.

Supplier: G-Biosciences

A synthetic anti-inflammatory glucocorticoid. Induces apoptosis in a variety of cell types1,2. It inhibits proliferation of A549 human lung adenocarcinoma cells and induces cell surface expression of lipocortin-13. Induces osteogenic differentiation of human mesenchymal stem cells4. Induces indoleamine-2,3-dioxygenase (IDO) and exerts IDO-dependent protection in a model of allergic airway inflammation5. Resets and synchronizes circadian clock in cultured cells6.

Supplier: G-Biosciences

Antimalarial drug. Inhibits autophagy in a variety of cell lines1. Induces cell death in breast cancer cell lines and displays antitumor and antimetastatic activity in mouse models of breast cancer2. Eliminates cancer stem cells via deregulation of Jak2 and D nMT13. Displays synergy when combined with the Raf inhibitor, vemurafenib, in brain tumor4. Cell permeable and active in vivo.

Supplier: G-Biosciences

An analog of sphingosine which, when taken up into cells, is phosphorylated and acts as an agonist at sphingosine-1-phosphate receptors (S1P1/3)1. Potently inhibits regulatory T cell proliferation in vitroand in vivo2. Enhances microvascular remodeling via expansion of arterioles and capillary networks3. Potent immunomodulatory, down-regulating microglial cell inflammation4. Clinically effective agent for relapsing forms of multiple sclerosis5. Stimulates neuronal gene expression, axonal growth and regeneration6.

Supplier: G-Biosciences

NADPH oxidase, NOX1/4 inhibitor (Ki = 100-150 nM).1 Inhibits erastin-stimulated ROS production.2 Potentiates immunotherapy by overcoming cancer-associated fibroblast- mediated CD8+ T-cell exclusion.3 Reduces ROS production in LPS-stimulated platelets in a mouse model.4 Reduces subarachnoid hemorrhage-induced neuronal death.5 Potent inhibitor of experimental liver fibrosis in mouse models.

Supplier: Thermo Scientific Chemicals

For iodination of proteins that do not contain tyrosine residues

Supplier: Edvotek

Immunodiffusion procedure makes results easy to visualize.

Supplier: Rockland Immunochemical

Lyophilized Non-Sterile Serum

Supplier: G-Biosciences

RORγt is a T cell specific isoform of ROR that is a key transcription factor for initiating the differentiation of Th17 and Tc17 cells. SR0987 (303126-97-8) is a RORγt agonist that drives proliferation of Th17 cells and decrease levels of the immune checkpoint protein PD-1. In a Ga14 UAS-Luc cotransfection system, SR0987 induced reporter gene expression (EC50=800 nM). Treatment of human Jurkat T cells with SR0987 resulted in decreased cell surface PD-1 expression. RORγt agonists may synergize with current anti-PD-1 therapy and represent a new potential class of cancer immunotherapeutic.

Supplier: G-Biosciences

Ibrutinib (936563-96-1) is a very potent (IC50 = 0.5 nM) irreversible inhibitor of Bruton tyrosine kinase (BTK) that blocks activation of the B-cell antigen receptor (BCR).1 Ibrutinib also potently inhibits several other kinases including BLK, BMS, FGR, EGFR, and ITK. It is a clinically useful drug to treat B cell cancers. It inhibits CLL cell migration and survival2,3 and downregulates expression of CD204. It enhanced antitumor immune responses in combination with anti PD-L1 blockade via its inhibition of ITK (IL2-inducible T-cell kinase).

Supplier: G-Biosciences

Muscone is a macrocyclic ketone found naturally in a glandular secretion of the musk deer which acts at human odorant receptor OR5AN1 and is used in perfumes1. It suppresses inflammatory responses and neuronal damage in various models including a rat model of cervical spondylotic myelopathy2 and relieves inflammatory pain acting via the NOX4/JAK2-STAT3 and NLRP3 inflammasome pathway3. In the cardiovascular system it alleviates myocardial ischemia-reperfusion injury4 and improves cardiac function in mice after myocardial infarction5 via enhancement of angiogenesis6.

Supplier: G-Biosciences

A specific competitive inhibitor of nucleotide binding to Rab7, an endosome-localized small GTPase1. It interferes with the Rab7-RILP interaction, locking the GTPase in an inactive conformation2. Impairs B cell class switching and plasma cell survival dampening, the pathogenic autoantibody response in lupus3. Inhibits the Arf-like small GTPase Arl8b, blocking cellular egress of β-coronaviruses (SARS-CoV-2) which utilize a lysosomal exocytosis pathway4.

Supplier: G-Biosciences

Selective inhibitor of Rho kinase 2 (ROCK2) – IC50’s: ROCK2=105 nM, ROCK1=24 µM in enzyme assays.1 It downregulated the ability of T cells to secrete IL-21 and IL-17, but not IFN-γ, in response to T cell stimulation in vitro via a STAT3-dependent mechanism.2 Belumosudil ameliorated graft-versus-host disease in multiple models3,4 and was recently approved by the FDA for the treatment of GVHD (July 2021). It has also been shown to suppress adipogenesis.5-7 Suppression of adipocyte differentiation is mediated via casein kinase 2 inhibition (IC50=50 nM) and not ROCK2 inhibition.

Supplier: G-Biosciences

I-BET762 is a potent inhibitor of the BET family of bromodomains with no activity at bromodomains BAZ2B, SP140, ATAD2, CREBBO, and PCAF.1,2 IC50’s for H4Ac peptide displacement: BRD2 = 32.5 nM, BRD3 = 42.4 nM, BRD4 = 36.1 nM.1 It was able to suppress proinflammatory proteins by macrophage, block acute inflammation in mice1, and suppressed the inflammatory function of T cells3. Inhibition of BET bromodomains results in downregulation of Myc transcription, an important oncogene.

Supplier: G-Biosciences

Potent and selective inhibitor of cFMS receptor kinase. Completely inhibits cFMS in vitroat 60 nM with no activity against 26 other kinases1. Completely inhibits CSF-1 induced growth of mouse M-NFS-60 myeloid cells and human monocytes (at 1 μM)1. Completely inhibits bone degradation in human osteoclasts, rat calvaria and fetal long bone1. Inhibits LPS-induced TNF production in mice. Abrogates infiltration of macrophages into synovial joints of arthritic mice2. Active in vivo.