986 Results for: "Cellular & Molecular Biology"

Supplier: Cloud-Clone

Monoclonal Antibody to Insulin Receptor (INSR), derived from recombinant Anti-INSR (Arg1027~Met1364), is reactive with Human.

Supplier: Tonbo Biosciences

7-AAD (7-Aminoactinomycin D) is a nucleic acid dye that can be used to exclude nonviable cells from the analysis of flow cytometry data based on light scatter and fluorescence properties.

Supplier: Stemcell Technologies

CFDA-SE (5(6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE.

Supplier: Thermo Scientific Chemicals

Neomycin sulfate, 50 mg/ml in distilled water, sterile-filtered

Supplier: Tonbo Biosciences

Propidium Iodide (PI) is a membrane-impermeant DNA binding dye that cannot penetrate viable cells. PI rapidly enters cells with compromised membranes and intercalates between base pairs allowing exclusion of non-viable cells from analysis of flow cytometry data. PI has a broad emission spectrum from 535-617 nm and can be detected in either FL2 or FL3 detectors. When used with Annexin V FITC, it is recommended to analyze PI in FL2.

Supplier: Cloud-Clone

Polyclonal antibody to Insulin Receptor (INSR), derived from recombinant Anti-INSR(Ser1114~Pro1382), is reactive with Rat/Human/Mouse.

Supplier: Stemcell Technologies

A cell-permeable calcium dye that exhibits fluorescence upon increasing Ca2+ concentrations.

Supplier: MP Biomedicals

Arachidonic Acid is an essential fatty acid. Occurs in liver, brain, glandular organs, and depot fats of animals, in small amounts in human depot fats, and is a constituent of animal phosphatides.
Arachidonic Acid is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes. Arachidonic acid plays a key role in cellular regulation and is controlled through multiple interconnected pathways.
Arachidonic acid (AA) is an unsaturated ω6 fatty acid constituent of the phospholipids of cell membranes. Phospholipase A2 releases AA from the membrane phospholipids in response to inflammation. AA is subsequently metabolized to prostaglandins and thromboxanes by at least two cyclooxygenase (COX) isoforms, to leukotrienes and lipoxins by lipoxygenases, and to epoxyeicosatrienoic acids via cytochrome p450-catalyzed metabolism. AA and its metabolites play important roles in a variety of biological processes, including signal transduction, smooth muscle contraction, chemotaxis, cell proliferation and differentiation, and apoptosis. AA has been demonstrated to bind to the a subunit of G protein and inhibit the activity of Ras GTPase-activating proteins (GAPs). Cellular uptake of AA is energy dependent and involves protein-facilitated transport across the plasma membrane.
If ethanol is undesirable, arachidonic acid may be dissolved in acetonitrile, DMF, or DMSO. Simply evaporate the ethanol under a gentle stream of nitrogen (be certain not to evaporate the material to dryness) and redissolve the arachidonic acid in the solvent of choice.Just prior to use, make dilutions of the stock solution into aqueous buffer or isotonic saline to bring the arachidonic acid to the desired concentration. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiologic effects at low concentrations. A control using the solvent in the absence of the prostaglandin will address this potential variable. We do not recommend storing the aqueous solution for more than one day. It is difficult to obtain aqueous solutions of arachidonic acid directly. However, an organic solvent free solution of arachidonic acid can be prepared using concentrated basic buffers (pH > 8.0 and ionic strength not less than 0.1 M). Add 400 μL of cold buffer (0 °C) per mg of arachidonic acid and agitate vigorously and/or ultrasonicate.

Supplier: Thermo Scientific Chemicals

Kanamycin, 50 mg/mL in distilled water, sterile-filtered.

Supplier: Cloud-Clone

This assay has high sensitivity and excellent specificity for detecting Human Anti-INSR (Insulin Receptor). The assay range is from 3.12 to 200 ng/ml (Competitive kit) with a sensitivity of 1.27 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

Supplier: AAT Bioquest

7-Amino actinomycin D (7-AAD) is a non-permeant dye that can be used to identify non-viable cells.

Supplier: Cloud-Clone

This assay has high sensitivity and excellent specificity for detecting Mouse Anti-INSR (Insulin Receptor). The assay range is from 0.312 to 20 ng/ml (Sandwich kit) with a sensitivity of 0.122 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

Supplier: Biotium

MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.

Supplier: Cloud-Clone

Polyclonal Antibody to Insulin Receptor (INSR), derived from recombinant Anti-INSR(Arg1027~Met1364), is reactive with Human/Mouse/Rat/Pig.

Supplier: Biotium

MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.

Supplier: Thermo Scientific Chemicals

Paromomycin sulfate in distilled water and sterile-filtered.

Supplier: Biotium

MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.

Supplier: AAT Bioquest

Although Lissamine Rhodamine B chloride is one of the most popular labeling reagents of sulfonyl chloride, it is quite unstable in water. Even in anhydrous DMF, Lissamine Rhodamine B chloride tends to give a very complicated reaction mixture. It reacts with thiols, alcohols, phenols, aliphatic amines and aromatic amines indiscriminatingly. LRB Red™ is a succinimidyl ester and has the spectral properties identical to those of Lissamine Rhodamine B chloride. LRB Red™ is a superior replacement for Lissamine Rhodamine B chloride. LRB Red™ only reacts with aliphatic amines such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable. Compared to Lissamine Rhodamine B chloride, LRB Red™ has much higher labeling efficiency, and more importantly the resulted conjugates are much easier to be purified due to its much cleaner reactions. We strongly recommend that you consider using LRB Red™ to replace Lissamine Rhodamine B chloride for labeling peptides and oligonucleotides.

Supplier: Stemcell Technologies

Propidium Iodide (PI) is a red-fluorescent cell viability dye which is excluded from live cells with intact membranes, but penetrates dead or damaged cells and binds to DNA and RNA by intercalating between the bases.

Supplier: Thermo Scientific

The Thermo Scientific Megafuge™ 8 centrifuge provides exceptional capacity in a compact design with a smart, simple interface and the flexibility to support both clinical and research applications. The centrifuge fits in to space, with its compact footprint, and to applications, accomodating multi-laboratory settings.

Supplier: Thermo Scientific Chemicals

Potent inhibitor of protein synthesis in bacteria

Supplier: Biotium

NucSpot® Far-Red was designed to be an improved alternative to 7-AAD showing less bleed-through fluorescence in the PE-Texas Red® channel. Ideal for selective detection of dead cells by flow cytometry.

Supplier: MP Biomedicals

Propidium iodide is a fuorescent stain for nucleic acids.

Supplier: Thermo Scientific Chemicals

Apramycin sulfate binds to the deep groove of RNA and effectively inhibits ribosomal translocation prohibiting protein synthesis.

Supplier: Stemcell Technologies

7‐AAD (7‐Aminoactinomycin D) is a fluorescent cell viability dye which is excluded from live cells with intact membranes but penetrates dead or damaged cells and binds to double‐stranded DNA with high affinity by intercalating between GC base pairs.

Supplier: Molecular Devices

The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining imaging and powerful analysis for individual labs that need easy automated imaging solutions. Image modes: Transmitted light (brightfield), colorimetric, fluorescence.